Molecular Characterization and Phylogenetic Analysis of VP1 of Porcine Enteric Picornaviruses Isolates in Italy

Sozzi, Enrica; Barbieri, Ilaria; Llombart‐Bosch, Antonio; Lelli, Davide; Moreno, Ana; Canelli, Elena; Bugnetti, M.; Cordioli, Paolo

doi:10.1111/j.1865-1682.2010.01170.x

Cited by 53 publications

(33 citation statements)

References 19 publications

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“…PEVs have been isolated from swine having asymptomatic infections in many regions including Italy [46]. In this study, the detection of PEVs in all Bulacan towns (rural) and in Las Pinas (urban) river waters demonstrates the possibility that viruses come from swine farms that abound in both areas.…”

Section: Discussionmentioning

confidence: 66%

Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines

et al. 2012

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Despite the vast distribution and expansive diversity of enteroviruses reported globally, indicators defining a complete view of the epidemiology of enteroviruses in tropical countries such as the Philippines are yet to be established. Detection of enteroviruses in the environment has been one of the markers of circulating viruses in a community. This study aimed to bridge the gap in the epidemiology of enteroviruses in the Philippines by providing an overview of the occurrence of enteroviruses in both urban and rural rivers. Molecular detection directed at the VP1 region of the enterovirus genome was performed on 44 grab river water samples collected from April to December 2009. The majority of the enterovirus serotypes detected were clustered with human enterovirus C species (HEV-C; 21/42), followed by HEV-B (12/42) and HEV-A (9/42). Porcine enterovirus 9 was also found in 12 out of 44 water samples. Phylogenetic analysis indicated that the viruses detected were closely related, if not all forming a monophyletic clade, with those enteroviruses detected previously from acute flaccid paralysis cases in the country. The clustering of environmental and human enterovirus strains implies that the circulation of these strains were associated with river contamination. This study gives further evidence of the environmental persistence of enteroviruses once they are shed in feces and likewise, provides additional data which may help in understanding the epidemiology of enteroviruses in humans, highlighting the need for more studies on the potential public health risks linked with enteroviruses found in the environment and their eventual clinical consequences in the country. Electronic supplementary material The online version of this article (doi:10.1007/s11262-012-0776-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 66%

Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines

et al. 2012

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“…The overall detection frequency of EVs in domestic pigs (81.6 %) was notably higher than those reported in Spain (0 %), Italy (7.5 %), China (8.3 %) and the Czech Republic (50.2 %) (Buitrago et al, 2010;Prodělalová, 2012;Sozzi et al, 2010;Yang et al, 2013). In contrast, detection of EV RNA in boars in this study was much lower than the 50 and 69.4 % prevalence reported in Hungary (Boros et al, 2012a) and the Czech Republic (Prodělalová, 2012), respectively.…”

Section: Discussionmentioning

confidence: 97%

Large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in Vietnam

Dũng

Anh

Cương

et al. 2016

Journal of General Virology

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A recent survey of pigs in Dong Thap province, Vietnam identified a high frequency of enterovirus species G (EV-G) infection (144/198; 72.7 %). Amongst these was a plethora of EV-G types (EV-G1, EV-G6 and four new types EV-G8-EV-G11). To better characterize the genetic diversity of EV-G and investigate the possible existence of further circulating types, we performed a larger-scale study on 484 pig and 45 farm-bred boar faecal samples collected in 2012 and 2014, respectively. All samples from the previous and current studies were also screened for kobuviruses. The overall EV infection frequency remained extremely high (395/484; 81.6 %), but with comparable detection rates and viral loads between healthy and diarrhoeic pigs; this contrasted with less frequent detection of EV-G in boars (4/45; 8.9 %). EV was most frequently detected in pigs j14 weeks old (,95 %) and declined in older pigs. Infections with EV-G1 and EV-G6 were most frequent, whilst less commonly detected types included EV-G3, EV-G4 and EV-G8-EV-G11, and five new types (EV-G12-EV-G16). In contrast, kobuvirus infection frequency was significantly higher in diarrhoeic pigs (40.9 versus 27.6 %; P50.01). Kobuviruses also showed contrasting epizootiologies and age associations; a higher prevalence was found in boars (42 %) compared with domestic pigs (29 %), with the highest infection frequency amongst pigs .52 weeks old. Although genetically diverse, all kobuviruses identified belonged to the species Aichivirus C. In summary, this study confirms infection with EV-G was endemic in Vietnamese domestic pigs and exhibits high genetic diversity and extensive inter-type recombination.

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“…To characterize the complete full-length genome sequences of each strain, ten primer sets (Table S1 in File S1) were designed to amplify the complete ORF sequences of each PSV strain based on the published genomic sequences of the PSV-V13 (GenBank ID: NC_003987), csh (GenBank ID: HQ875059) and YC2011 strains (GenBank ID: JX286666). Standard one-step RT-PCR assays were performed as previously described [25].…”

Section: Methodsmentioning

confidence: 99%

“…Although PSVs can be important pathogens because of their wide distribution and high prevalence [21]–[25], the near-complete genomic sequences of only three PSV strains have been reported previously; one from the U.K. (V13 strain) and two from China (csh and YC2011 strains). This prompted us to characterize the full-length genetic properties of Korean PSV strains in comparison with those of other known PSV strains.…”

Section: Introductionmentioning

confidence: 99%

Full-Length Genomic Analysis of Korean Porcine Sapelovirus Strains

et al. 2014

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Porcine sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3′ poly(A) tail, and showed the typical picornavirus genome organization; 5′untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3′UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5′UTR, a cis-replication element (CRE) in the 2C coding region and 3′UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3′UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.

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Molecular Characterization and Phylogenetic Analysis of VP1 of Porcine Enteric Picornaviruses Isolates in Italy

Cited by 53 publications

References 19 publications

Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines

Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines

Large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in Vietnam

Full-Length Genomic Analysis of Korean Porcine Sapelovirus Strains

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