Molecular Characterization and Pathology of Virulent Newcastle Disease Virus Isolated from Broiler Chickens in District Lahore, Pakistan

Ali, Mortuza; Khan, M.U.R.; Aslam, Asim; Rehman, Habib ur; Anjum, A. D.; Waheed, Samina; Usman, S.; Masood, Sadia; Ullah, Ashik; Masood, Amjed

doi:10.18805/ijar.bf-1420

Cited by 1 publication

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“…Phylogenetic analysis of the reported sequences indicates that 3/6 sequences belong to genotype VII.1.1. These sequences clustered with previous detections from chickens in Iran, Pakistan, and Malaysia (32)(33)(34). Genotype VII was reported in commercial poultry in ERSA between 2012 and 2016 (22, 23).…”

Section: Discussionsupporting

confidence: 77%

Detection of Avian Orthoavulavirus-1 genotypes VI.2.1 and VII.1.1 with neuro-viscerotropic tropism in some backyard pigeons (Columbidae) in Eastern Saudi Arabia

Al-Mubarak,

Al-Kubati,

Sheikh

et al. 2024

Front. Vet. Sci.

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IntroductionAvian orthoavulavirus-1 (AOAV1) has a wide host range, including domestic and wild birds. The present study aimed to identify the currently circulating AOAV1 strains from some outbreaks in some backyard pigeons in the eastern region of Saudi Arabia (ERSA).MethodsTracheal/cloacal swabs and tissue specimens were collected from eight backyards in Al-Ahsa, ERSA, between January 2021 and March 2023. Samples were tested for the presence of AOAV1 using commercial real-time RT-PCR. Part of the fusion gene was also amplified by gel-based RT-PCR, and the obtained amplicons were sequenced.Results and discussionAOAV1 was detected in samples from the eight flocks. The retrieved sequences from samples of 6/8 pigeon backyards are reported. Phylogenetic analysis based on the obtained sequences from these backyard pigeons showed the segregation of the obtained sequences in AOAV1 genotypes VI.2.1 and VII.1.1. Clinically, nervous manifestations were dominant in pigeons infected with both genotypes. Respiratory manifestations and significantly higher overall mortality rate were induced by genotype VI.2.1. The deduced amino acid sequences of the fusion protein cleavage site (FPCS) showed that all the detected isolates belong to velogenic strains. Differences in clinical profiles induced by the natural infection of pigeons with AOAV1 genotypes VI.2.1 and VII.1.1 were reported. The present findings highlight the potential roles of some backyard pigeons in the long-distance spread and cross-species transmission of the reported AOAVI genotypes. Further research is required to perform biotyping and pathotyping of the reported strains.

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Section: Discussionsupporting

confidence: 77%