Molecular characterization and pathological identification of a novel strain of delta papillomavirus-4 (bovine papillomavirus-2) in Egypt

Abstract

Background and Aim: Bovine papillomaviruses (BPV) are a heterogeneous group of oncoviruses, distributed globally, which produce major economic losses. In the current study, we compared the results of different diagnostic approaches and compared the strains identified in this study with previously characterized strains at local and international levels. Materials and Methods: Samples of skin warts were collected from five bovines with generalized papillomatosis from two Egyptian provinces, Menya and Ismailia, in 2020. Electron microscopy, molecular characterization, histopathological, and immunohistochemical examination were performed. Results: BPV was detected using electron microscopy in the collected samples. Using molecular characterization, BPV-2 was successfully identified for 1st time in Egypt. The strain has 99.6% identity with the BPV-2 reference strains obtained from GenBank. These results were supported by histopathology and immunohistochemistry examination. Partial nucleotide sequences of the L1 gene were submitted to GenBank with accession numbers MW289843 and MW289844. Conclusion: BPV-2 was reported for 1st time in the current study. The strain was identified grossly, microscopically, and pathologically and confirmed using molecular approaches. All results were consistent. The sequence analysis revealed that this strain has high sequence similarity to the reference Deltapapillomavirus-4, BPV-2 strains from Brazil and China.

Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis

“…Although the clinical diagnosis of BP is usually performed when alterations are well characterized in the epidermis, histology and immunohistochemistry (IHC) for stained papilloma tissues can allow an identification of epidermal pathogenic alterations and reveal viral proteins, respectively ( Russo et al ., 2020 ; Hassanien et al ., 2021 ). Molecular techniques, in particular polymerase chain reaction (PCR), are important and essential diagnostic tools that are usually applied to confirm infection through the detection of specific DNA of BPV ( Emin et al ., 2022 ).…”