Molecular characterization and pathogenicity of a genogroup GVI feline norovirus

Takano, Tomomi; Kusuhara, Hajime; Kuroishi, Akira; Takashina, Midori; Doki, Tomoyoshi; Nishinaka, Takamichi; Hohdatsu, Tsutomu

doi:10.1016/j.vetmic.2015.05.018

Cited by 25 publications

(25 citation statements)

References 23 publications

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“…NoVs were detected in cats with enteric signs while they were not identified in samples collected from healthy animals used as control study group. Experimental inoculation of specific pathogen free cats with the feline GVI.1 strain JPN/2012/M49 can induce enteritis signs, diarrhea and vomiting (Takano et al, 2015). Although the pathogenic role of NoV in cats should be confirmed in larger epidemiological studies and, possibly, in experimental infections with other NoV genotypes, these findings seem to indicate a possible role of these viruses as feline enteric pathogens.…”

Section: Discussionmentioning

confidence: 97%

“…The canine NoV strain Bari/91/07/ITA, resembles GIV.2 NoVs in its polymerase gene while it is genetically unrelated in the VP1 gene to GIV NoV (Martella et al, 2009). The feline NoV strain, JPN/2012/M49 (Takano et al, 2015), possesses a GIV.2 RdRp region, and a GVI.1 ORF2 related to the canine virus Bari/91/07/ITA (87.0% aa identity). In all the cases, the site of recombination was mapped to the ORF1/ORF2 junction region.…”

Section: Discussionmentioning

confidence: 99%

“…1). The strain cat/TE/77-13/ITA was grouped with the feline NoV strains lion/GIV.2/Pistoia/387/06/ITA, cat/GIV.2/ CU081210E/USA/2010 and cat/GVI.1/JPN/2012/M49 (Martella et al, 2007;Pinto et al, 2012;Takano et al, 2015), with a nt identity of 90.8-94.0%. This group shared identity of 82.3-85.4% to the recombinant NoV dog/GVI.1/91/2007/ITA and to the GIV.2 strains dog/170/07/ ITA and dog/Thessaloniki/30/08/GR (Martella et al, 2008(Martella et al, , 2009Ntafis et al, 2010), which in turn segregated in a second cluster (91.6-97.8% nt identity Two additional clusters were resolved in the tree that included, respectively, the human GIV.1 strains detected from stool and sewage samples in different geographic settings (Fankhauser et al, 2002;La Rosa et al, 2008;Eden et al, 2012;Ao et al, 2014;Han et al, 2014) and GIV.1 NoVs found in sewage samples in Italy in -2009(La Rosa et al, 2010.…”

Section: Oligonucleotidementioning

confidence: 99%

“…In addition, the RNA of GIV.2 NoVs has been detected in 1.2% of fecal samples of cats with enteritis in Japan (Soma et al, 2014). Upon genome sequencing, the feline NoV strain cat/GVI.1/JPN/2012/M49 (Takano et al, 2015) was found to be more similar (87.0% aa identity) in the full-length ORF2 to the canine NoV GVI.1/Bari/91/07/IT (Martella et al, 2009). Infection, Genetics and Evolution 38 (2016)…”

Section: Introductionmentioning

confidence: 99%

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A novel feline norovirus in diarrheic cats

Martino

Profio

Melegari

et al. 2016

Infection, Genetics and Evolution

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By screening a collection of fecal samples from young cats housed in three different shelters in South Italy, noroviruses (NoVs) were found in 3/48 (6.2%) specimens of animals with enteritis signs while they were not detected in samples collected from healthy cats (0/57). Upon sequence analysis of the short RNA-dependent RNA polymerase (RdRp) region, the three strains displayed the highest nucleotide (nt) and amino acid (aa) identities to the prototype GIV.2 strain lion/Pistoia/387/06/ITA (91.0-93.0% nt and 97.0-98.0% aa). The sequence of ~3.4-kb portion at the 3' end of the genome of a NoV strain, TE/77-13/ITA, was determined. In the full-length ORF2, encoding the VP1 capsid protein, the virus was genetically closest to the canine GVI.2 NoV strains C33/Viseu/2007/PRT and FD53/2007/ITA (81.0-84.0% nt and 93.0-94.0% aa identities), suggesting a recombination nature, with the cross-over site being mapped to the ORF1-ORF2 junction. Based on the full-length VP1 amino acid sequence, we classified the novel feline NoV, together with the canine strains Viseu and FD53, as a genotype 2, within the genogroup GVI. These findings indicate that, as observed for GIV NoV, GVI strains may infect both the canine and feline host. Unrestricted circulation of NoV strains in small carnivores may provide the basis for quick genetic diversification of these viruses by recombination. Interspecies circulation of NoVs in pets must also be considered when facing outbreaks of enteric diseases in these animals.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Oligonucleotidementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A novel feline norovirus in diarrheic cats

Martino

Profio

Melegari

et al. 2016

Infection, Genetics and Evolution

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“…As shown in Martino et al, 2016;Martella et al, 2007;Pinto et al, 2012;Takano et al, 2015). (Table 4).…”

Section: Sequencing and Phylogenetic Analysismentioning

confidence: 91%

Identification of a novel canine norovirus

Bodnar

Lorusso

Martino

et al. 2017

Infection, Genetics and Evolution

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By screening a collection of fecal samples from young dogs from different European countries, noroviruses (NoVs) were found in 13/294 (4.4%) animals with signs of enteritis whilst they were not detected in healthy dogs (0/42). An informative portion of the genome (3.4kb at the 3' end) was generated for four NoV strains. In the capsid protein VP1 region, strains 63.15/2015/ITA and FD53/2007/ITA were genetically related to the canine GVI.2 strain C33/Viseu/2007/PRT (97.4-98.6% nt and 90.3-98.6% aa). Strain FD210/2007/ITA displayed the highest identity to the GVI.1 canine strain Bari/91/2007/ITA (88.0% nt and 95.0% aa). Strain 5010/2009/ITA displayed only 66.6-67.6% nt and 75.5-81.6% aa identities to the GVI.1 canine strains FD210/2007/ITA and Bari/91/2007/ITA and the GVI feline strain M49-1/2012/JPN. Identity to the other canine/feline NoVs strains in the VP1 was lower than 67.6% nt and 62.7% aa. Based on the full-length VP1 amino acid sequence and the criteria proposed for distinction of NoV genotypes, the canine NoV 5010/2009/ITA could represent the prototype of a third GVI genotype, thus providing further evidence for the genetic heterogeneity of NoVs in carnivores.

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