Molecular Characterization and Oxidative Stress Response of a Cytochrome P450 Gene (CYP4G11) from Apis cerana cerana

Abstract

Cytochrome P450 proteins, widely distributed multifunctional enzymes, are mainly involved in biosynthetic and degradative pathways of endogenous compounds and the detoxifi cation of xenobiotics in insects. Moreover, these enzymes exhibit peroxidase-like activity, therefore they may be involved in protecting organisms against the toxicity of reactive oxygen species (ROS). In the present study, we cloned a CYP4G11 gene -AccCYP4G11 -from the Chinese honey-bee (Apis cerana cerana). The open reading frame of the cDNA was 1656 bp long and encoded a 551 amino acids polypeptide, which shared high sequence identity with homologous cytochrome P450 proteins. In the genomic DNA sequence, a 5'-fl anking region consisting of 1168 bp was obtained, and some putative transcription factor binding sites were predicted. Quantitative polymerase chain reaction (Q-PCR) revealed that the level of AccCYP4G11 was higher in the epidermis than in other tissues, and Acc-CYP4G11 was expressed in all stages with the highest level in two-week-old adult worker honey-bees. More over, the expression patterns under oxidative stress indicated that Acc-CYP4G11 transcription was signifi cantly infl uenced by external factors, such as temperature challenges, ultraviolet (UV) light, and insecticide treatment. AccCYP4G11 was regulated differentially in response to oxidative stress and may be involved in protecting honey-bees from oxidative injury.

The Roles of Four Novel P450 Genes in Pesticides Resistance in Apis cerana cerana Fabricius: Expression Levels and Detoxification Efficiency

“…Currently, this topic has become a major focus and has led to widespread efforts to understand the molecular basis that underlies insecticide resistance. Previous researches identified and characterized several P450 genes and verified that those P450 genes responded to insecticide detoxification in A. c. cerana (Shi et al, 2013; Zhang et al, 2018). In this study, four novel P450 genes were identified and characterized.…”

“…In resistant Anopheles mosquitoes , the two P450 enzymes, CYP4G16 and CYP4G17 , are frequently overexpressed and particularly highly enriched in the abdominal integument (Ingham et al, 2014). Early studies provided evidence that the CYP4G11 gene plays an important role in protecting honeybees from insecticide (phoxim, paraquat, decamethrin) damage (Shi et al, 2013). The P450s in the honeybee genome play a crucial role in the detoxification of phytochemicals and pesticides in the diet (nectar, honey, and pollen) of both larval and adult stages (Mao et al, 2009).…”

Identification of a new P450s gene (AccCYP4AV1) and its roles in abiotic stress resistance in the Apis cerana cerana Fabricius

“…In many insects, P450s play important roles in detoxification, including endogenous and exogenous agents (Shen et al ., 2003; Sasabe et al ., 2004; Kirischian and Wilson, 2012). However, only a few P450s genes from A. c. cerana have been reported (Shi et al ., 2013; Zhu et al ., 2016; Zhang et al ., 2018). Therefore, the identification and characterization of new P450s genes have become very attractive research areas.…”

“…Early studies by Shi et al . (2013) provided evidence that the CYP4G11 gene was involved in protecting honeybee workers from abiotic stress damage. Several CYP4 genes are overexpressed in the pesticide-resistant Culex pipiens , Diabrotica virgifera , and Helicoverpa armigera species (Pittendrigh et al ., 1997; Scharf et al ., 2001; Shen et al ., 2003).…”

“…c . cerana (Shi et al ., 2013; Zhang et al ., 2018). However, the functions of AccCYP4AV1 during the response to abiotic stress in A. c. cerana have not been well understood, yet.…”