Molecular characterization and microbial resistance of different bacterial isolates in some dairy products

Alkhafaje, Waleed K.; Olama, Zakia A.; Ali, Safaa M.

doi:10.33899/ijvs.2021.130206.1764

Cited by 4 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The partial 16S rRNA gene sequence (265 bp) of the strain was collected from the vaginal swabs of the aborted ewes, and the two strands were sequenced. The sequence was compared with those in available databases using BLAST and aligned with its nearest neighbors using Mega X (31)(32)(33).…”

Section: Sequencing Of the 16s Rrna Gene And Phylogenetic Analysismentioning

confidence: 99%

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Arif

2022

IJVS

View full text Add to dashboard Cite

Abortion is one of the most critical factors affecting lambing rates and, as a result, sheep farm profitability. It is also significant from a zoonotic viewpoint, in addition to financial losses. In sheep flocks, Campylobacter fetus causes infectious infertility, embryonic death, and miscarriages. The study investigated C. fetus from aborted fetuses and vaginal swab samples collected from sheep flocks in the Sulaimani province by the polymerase chain reaction. Thirty-eight aborted fetuses and 70 vaginal swabs were collected from sheep flocks in three districts of Sulaimani province (Kalar, Said Sadiq, and Chamchamal) from March 2018 to June 2019. The pathogen was identified in clinical specimens using conventional PCR. C. fetus was isolated in 16 of 38 aborted fetuses 42.1% and 13 of 70 vaginal swabs from aborted ewes 18.6%. The C. fetus gene 16S rRNA was sequenced and received the accession number MW694741 in NCBI GenBank. Phylogenetic analysis of 16S rRNA gene sequences designated that the C. fetus isolates formed a separate branch displayed the highest similarity and clustered with MN203686.1 and EU773268.1 accessions in a specific clade. A lower degree of affinity of C. fetus was revealed with Campylobacter coli and Campylobacter jejuni.

show abstract

Section: Sequencing Of the 16s Rrna Gene And Phylogenetic Analysismentioning

confidence: 99%

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Arif

2022

IJVS

View full text Add to dashboard Cite

show abstract

“…Recently, ARGs classified as microcontaminants that might hurt animal and human health. 24,25 Further researches are urgently needed to prevent the dissemination of ARGs to the environment. Find efficient methods for inactivation of ARGs in animal wastes, manure, and hospital wastes to reduce of emergence and dissemination of ARGS in the natural environments.…”

Section: Discussionmentioning

confidence: 99%

“…Find efficient methods for inactivation of ARGs in animal wastes, manure, and hospital wastes to reduce of emergence and dissemination of ARGS in the natural environments. 23,25…”

Section: Discussionmentioning

confidence: 99%

Using of Integrons as Biomarker to Assess Dissemination and Diversity of Antimicrobial Resistance Genes in Farm Animal Manure

Al-Ubaidy,

Alsultan

2023

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Antimicrobial agents are widely used for treatment of animal and human diseases. Heavy use of antimicrobial agents permits bacteria to develop resistance to these agents specifically when a dose of antibiotic is insufficient or course of treatment is incomplete. Antimicrobial resistance genes (ARGs) are usually associated with mobile genetic elements (MGEs) including Integron therefore; these genes can transmit among bacteria via horizontal transmission. The current study was conducted to assess the possible role of manure in dissemination of antimicrobial resistance. The presence, quantitate, and diversity of resistance genes associated with Integron class 1 have been assessed using conventional and quantitative polymerase chain reaction (PCR) combined with sequencing of gene cassette within Integron and analysis of sequenced data by blast tool. Thirty-eight samples were found a positive for Integron and concentration of Integron in positive sample ranged from from 106-1010 copies/g of manure. High frequencies were detected to genes that encoded to sulphonamide and ammonium compound resistance. These genes were detected in 25% and 23% respectively of the total manure samples. In general, the detected genes in manure functionally belong to five protein families including Efflux pump, DNA repair, heavy metal resistance, membrane protein, and antibiotic resistance. Manure might act as a hotspot from which ARGs emerge and transfer to the environment and then to the animal and human environments.

show abstract

“…The condition of the cyclical reaction was 4 min at 95°C and then 40 cycles of 40 s at 94°C, 50 s at 55°C, and 50 s at 72°C, monitored by an additional 10 min at 72°C. PCR reactions were run on an agarose gel, and the remaining mixture was purified for sequence ( Alkhafaje et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Characterization of an isolated lactase enzyme produced by Bacillus licheniformis ALSZ2 as a potential pharmaceutical supplement for lactose intolerance

Amin,

Olama,

Ali

2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

IntroductionLactose intolerance is a widespread problem that affects people of many different races all over the world. The following pharmacological supplements can improve the lives of those who suffer from this issue.MethodsThis work focused on lactase producer isolation and statistical design (Plackett–Burman, and BOX–Behnken) to maximize the effectiveness of environmental factors. A lactase-producing bacterium was chosen from a discovery of 100 strains in soil that had previously been polluted with dairy products. Plackett-Burman investigated fifteen variables.ResultsThe most critical variables that lead to increased lactase synthesis are glucose, peptone, and magnesium sulfate (MgSO4). The ideal process conditions for the creation of lactase yield among the stated variables were then determined using a BOX-Benken design. To establish a polynomial quadratic relationship between the three variables and lactase activity, the Box–Behnken design level was used. The EXCEL-solver nonlinear optimization technique was used to predict the best form for lactase production. The ideal temperature and pH levels have been determined, both before and after the lactase purification process, to achieve the highest performance of isolated lactase.ConclusionAccording to this study, Bacillus licheniformis is a perfect supply of the lactase enzyme (β -Galactosidase), It can be used as a product to assist people who have health issues due to lactose intolerance.

show abstract

Molecular characterization and microbial resistance of different bacterial isolates in some dairy products

Cited by 4 publications

References 0 publications

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Using of Integrons as Biomarker to Assess Dissemination and Diversity of Antimicrobial Resistance Genes in Farm Animal Manure

Characterization of an isolated lactase enzyme produced by Bacillus licheniformis ALSZ2 as a potential pharmaceutical supplement for lactose intolerance

Contact Info

Product

Resources

About