Molecular characterization and its antioxidant activity of a newly isolated Streptomyces coelicoflavus BC 01 from mangrove soil

Rao, Koppaka V.; Rao, T. Raghava

doi:10.1016/j.jyp.2013.10.002

Cited by 28 publications

(19 citation statements)

References 27 publications

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“…AM-S1 isolated from forest humus soil in Gyeongsan, South Korea where ethyl acetate extract exhibited higher activity as compared to the lyophilised cell-free supernatant. According to Rao and Rao (2013) , the extracts of Actinobacteria isolated from mangrove soil of Vishakhapatnam region showed 46–70% DPPH scavenging activity and 68–78% FRAP activity at 20 μg/mL concentration. Karthik et al (2014) reported an extracellular protease produced by a marine Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it was proven that the ocean floor possesses many unique forms of Actinobacteria ( Fenical and Jensen, 2006 ). Actinobacteria are widely distributed in intertidal zones, mangroves, seawaters, animals, plants, sponges, and in ocean sediments ( Goodfellow and Williams, 1983 ; Castillo et al, 2005 ; Jensen and Mafnas, 2006 ; Ramesh and Mathivanan, 2009 ; Sun et al, 2010 ; Xiao et al, 2011 ; Rao and Rao, 2013 ). Actinobacteria from the marine environment are considered as a promising source of pharmaceutically important compounds because of a different kind of unique adaptation characteristics ( Fenical and Jensen, 2006 ; Jose and Jha, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

et al. 2017

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Bacterial secondary metabolites possess a wide range of biologically active compounds including antibacterial and antioxidants. In this study, a Gram-positive novel marine Actinobacteria was isolated from sea sediment which showed 84% 16S rRNA gene sequence (KT588655) similarity with Streptomyces variabilis (EU841661) and designated as Streptomyces variabilis RD-5. The genus Streptomyces is considered as a promising source of bioactive secondary metabolites. The isolated novel bacterial strain was characterized by antibacterial characteristics and antioxidant activities. The BIOLOG based analysis suggested that S. variabilis RD-5 utilized a wide range of substrates compared to the reference strain. The result is further supported by statistical analysis such as AWCD (average well color development), heat-map and PCA (principal component analysis). The whole cell fatty acid profiling showed the dominance of iso/anteiso branched C15–C17 long chain fatty acids. The identified strain S. variabilis RD-5 exhibited a broad spectrum of antibacterial activities for the Gram-negative bacteria (Escherichia coli NCIM 2065, Shigella boydii NCIM, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas sp. NCIM 2200 and Salmonella enteritidis NCIM), and Gram-positive bacteria (Bacillus subtilis NCIM 2920 and Staphylococcus aureus MTCC 96). Extract of S. variabilis strain RD-5 showed 82.86 and 89% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and metal chelating activity, respectively, at 5.0 mg/mL. While H2O2 scavenging activity was 74.5% at 0.05 mg/mL concentration. Furthermore, polyketide synthases (PKSs types I and II), an enzyme complex that produces polyketides, the encoding gene(s) detected in the strain RD-5 which may probably involve for the synthesis of antibacterial compound(s). In conclusion, a novel bacterial strain of Actinobacteria, isolated from the unexplored sea sediment of Alang, Gulf of Khambhat (Gujarat), India showed promising antibacterial activities. However, fractionation and further characterization of active compounds from S. variabilis RD-5 are needed for their optimum utilization toward antibacterial purposes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

et al. 2017

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“…Thus, alternative antioxidants from natural sources are more preferable and many recent studies have shown that besides plants as rich source of antioxidants (Wong et al, 2012 ; Tan et al, 2015 ), microorganisms can be used for the production of natural antioxidants. Recently, many studies reported that mangrove Streptomyces produced antioxidative agents (Rao and Rao, 2013 ; Ser et al, 2015a ).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated from Malaysia Mangrove Soil

et al. 2015

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A Streptomyces strain, MUM256 was isolated from Tanjung Lumpur mangrove soil in Malaysia. Characterization of the strain showed that it has properties consistent with those of the members of the genus Streptomyces. In order to explore the potential bioactivities, extract of the fermented broth culture of MUM256 was prepared with organic solvent extraction method. DPPH and SOD activity were utilized to examine the antioxidant capacity and the results have revealed the potency of MUM256 in superoxide anion scavenging activity in dose-dependent manner. The cytotoxicity of MUM256 extract was determined using cell viability assay against 8 different panels of human cancer cell lines. Among all the tested cancer cells, HCT116 was the most sensitive toward the extract treatment. At the highest concentration of tested extract, the result showed 2.3-, 2.0-, and 1.8-folds higher inhibitory effect against HCT116, HT29, and Caco-2 respectively when compared to normal cell line. This result has demonstrated that MUM256 extract was selectively cytotoxic toward colon cancer cell lines. In order to determine the constituents responsible for its bioactivities, the extract was then subjected to chemical analysis using GC-MS. The analysis resulted in the identification of chemical constituents including phenolic and pyrrolopyrazine compounds which may responsible for antioxidant and anticancer activities observed. Based on the findings of this study, the presence of bioactive constituents in MUM256 extract could be a potential source for the development of antioxidative and chemopreventive agents.

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“…Extract from Streptomyces sp. SRDP‐H03 and Streptomyces coelicoflavus BC 01 isolated from soil also scavenged the DPPH. The IC 50 value of protocatechualdehyde produced from Streptomyces lincolnenis M‐20 was 0.2 mmol L −1 and ED 50 value of surugapyrone A produced from Streptomyces coelicoflavus strain USF‐6280 was 4.2 mmol L −1 [54].…”

Section: Resultsmentioning

confidence: 97%

Development of actinobacterial resources for functional cosmetics

Dahal

Shim

Kim

2017

J of Cosmetic Dermatology

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Molecular characterization and its antioxidant activity of a newly isolated Streptomyces coelicoflavus BC 01 from mangrove soil

Cited by 28 publications

References 27 publications

Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated from Malaysia Mangrove Soil

Development of actinobacterial resources for functional cosmetics

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