Molecular characterization and interactome analysis of Trypanosoma cruzi tryparedoxin II

Role of Proteomics in the Study of Trypanosoma cruzi Biology

“…This approach allowed the identification of 16 TcTXNII-interacting proteins that are involved in a wide range of cellular processes. Thus, this report indicated the relevance of TcTXNII and contributed to a better understanding of the T. cruzi redox interactome [77].…”

“…This allowed us to identify 15 TcTXN1 proteins that were involved in 2 main processes: oxidative metabolism and protein synthesis and degradation. This type of experiment allowed the discovery of several putative TcTXN1-interacting proteins and contributed to a better understanding of the redox T. cruzi interactome [77]. In another work, the study was extended to TcTXNII.…”

Redox Balance Keepers and Possible Cell Functions Managed by Redox Homeostasis in Trypanosoma cruzi

“…For example, TXN-I was predicted to interact with other oxidative metabolism components, cysteine and methionine related pathways, as well as protein translation and degradation partners (Piñeyro et al, 2011b). For TXN-II, different interactors were identified under physiological and oxidative stress conditions, and it was suggested to influence the proteins involved in energy metabolism and cytoskeleton and protein translation (Arias et al, 2015;Dias et al, 2018). Further studies will be required to demonstrate the biological significance of TXNs in T. cruzi adaptation to different environmental conditions imposed by its life cycle.…”

“…Further analysis of the interacting partners of the antioxidant components could give us a deeper knowledge of the pathways depending on redox balance. If we consider the available interactomes in trypanosomes (Piñeyro et al, 2011b;Arias et al, 2015;Peloso et al, 2016), it is clear that antioxidant system is linked to many cellular pathways. It is our hope that future investigations will focus on understanding the crosstalk between antioxidants and other cellular processes and enhance our understanding of the influence of redox balance on T. cruzi biology.…”

Protein Promiscuity in H2O2Signaling

“…For proteomic characterization of reversible disulfides with physiological significance, thioredoxin (Trx) has recently been utilized as a tool for specific capture of cellular redox targets (8,206,254,278,283,421). Both the tandem mass tag (TMT) and ICAT proteomic methods have been adapted to identify Trx targets, merely by using Trx as the postalkylation reductant instead of a chemical reducing agent, such as DTT or tris(2-carboxyethyl)phosphine (TCEP) (278,421).…”