Molecular characterization and immunogenicity of neutralization-sensitive Babesia bigemina merozoite surface proteins

McElwain, Terry F.; Perryman, Lance E.; Musoke, A.J.; McGuire, Travis C.

doi:10.1016/0166-6851(91)90181-5

Cited by 90 publications

(75 citation statements)

References 21 publications

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“…However, cattle immunized with DNA vaccine were also protected against challenge infection although specific antibodies were not detected. Similar observation was also taken in bovine babesiosis in which there was little correlation between antibody titers and protective immunity in animals immunized with parasite antigens or animals that had recovered from infection [16,21,23,24]. It is reported that proliferative responses of peripheral blood mononuclear cells (PBMC) against purified piroplasm of T. sergenti were detected in infected animals [25].…”

supporting

confidence: 60%

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Kakuda

Sugimoto

Onuma

2001

The Journal of Veterinary Medical Science

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ABSTRACT. The cellular immune responses against major piroplasm surface protein (MPSP) of Theileria sergenti were characterized. Three cattle were immunized with recombinant MPSP (C type) encapsulated by mannan-coated liposome. The proliferative responses of peripheral blood mononuclear cells (PBMC) against MPSP were detected in all immunized animals. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that T cell lines derived from each animal expressed relatively high levels of interferon (IFN)-γ mRNA, low levels of interleukin (IL)-2, IL-10, and tumor necrosis factor (TNF)-α mRNAs, but no detectable level of IL-4 mRNA. From the results of T cell epitope-mapping, T-cell lines from two animals responded to DTSKFTPTVAHRLKHAEDLF (residues 61 to 80), while other animal responded to GTGKVYDFVGNFKVTKVKFE (residues 141 to 160). The MPSP-type specific response of a Tcell line was observed in the latter region of MPSP. These data suggest that immunization with cocktail vaccine consisting of different types of MPSP may be necessary in the field trial. KEY WORDS: epitope-mapping, T-cell line, Theileria sergenti.

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confidence: 60%

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Kakuda

Sugimoto

Onuma

2001

The Journal of Veterinary Medical Science

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“…Immunization with affinity-purified native gp45 from the Mexico strain induces protection against homologous challenge, defined as a significant decrease in peak parasitemia compared to adjuvant-inoculated control cattle (21). However gp45, like B. bovis MSA-1, is antigenically polymorphic among strains (21,27).…”

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confidence: 99%

Molecular Basis for Variable Expression of Merozoite Surface Antigen gp45 among American Isolates ofBabesia bigemina

2001

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Immunization with the merozoite surface glycoprotein gp45 induces protection against challenge using the homologous Babesia bigemina strain. However, gp45 B-cell epitopes are highly polymorphic among B. bigemina strains isolated from different geographical locations within North and South America. The molecular basis for this polymorphism was investigated using the JG-29 biological clone of a Mexico strain of B. bigemina and comparison with the Puerto Rico, St. Croix, and Texcoco strains. The molecular size and antibody reactivity of gp45 expressed by the JG-29 clone were identical to those of the parental Mexico strain. gp45 cDNA and the genomic locus encompassing gp45 were cloned and sequenced from JG-29. The locus sequence and Southern blot data were consistent with a single gp45 copy in the JG-29 genome. The JG-29 cDNA expressed the full-length protein recognized by the gp45-specific monoclonal antibody 14/1.3.2. The genomes of the Puerto Rico and St. Croix strains of B. bigemina were shown to lack a closely related gp45-like gene by PCR using multiple primer sets and by Southern blots using both full-length and region-specific gp45 probes. This genomic difference was confirmed using unpassaged isolates from a 1999 disease outbreak in Puerto Rico. In contrast, the Texcoco strain retains a gp45 gene, encoding an open reading frame identical to that of JG-29. However, the Texcoco gp45 gene is not transcribed. These two mechanisms, lack of a closely related gp45-like gene and failure to transcribe gp45, result in generation of antigenic polymorphism among B. bigemina strains, and the latter mechanism is unique compared to prior mechanisms of antigenic polymorphism identified in babesial parasites.

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“…All rap-1 family members are co-transcribed in merozoites, and some members seem to be conserved in geographical strains [49]. It has been demonstrated in B. bovis that specific antibodies are capable of reducing sporozoite invasion to red blood cells in vitro [53]; in B. bigemina, antibodies against RAP-1 reduced parasitemia in comparison with an ovalbumin control in calves inoculated with iRBC [74].…”

Section: Rap-1mentioning

confidence: 99%

“…Some studies proved that immunization of calves with the purified GP45 reduced significantly the parasitemia when Farm Animals Diseases, Recent Omic Trends and New Strategies of Treatmentchallenged with a Mexican B. bigemina isolate [74]. At this point, GP45 seemed to be an ideal vaccine candidate, however, several years later, other studies demostrated that the gene is not present among all B. bigemina strains.…”

Section: Gp45mentioning

confidence: 99%

Genome-Based Vaccinology Applied to Bovine Babesiosis

Mosqueda¹,

Hernández-Silva²,

MarioHidalgo-Ruiz³

2018

Farm Animals Diseases, Recent Omic Trends and New Strategies of Treatment

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Genomics approaches in veterinary research have been a very useful tool to identify candidates with potential to be used in prevention of animal diseases. In Babesia, genome information analysis has elucidated a wide variety of protein families and some members are described in this chapter. Here, we present some of the most recent studies about B. bovis and B. bigemina genomes where some proteins have been identified with potential to prevent infections by these parasites.

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Molecular characterization and immunogenicity of neutralization-sensitive Babesia bigemina merozoite surface proteins

Cited by 90 publications

References 21 publications

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Molecular Basis for Variable Expression of Merozoite Surface Antigen gp45 among American Isolates ofBabesia bigemina

Genome-Based Vaccinology Applied to Bovine Babesiosis

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