Molecular characterization and genetic diversity analysis in Indian mustard (Brassica juncea L. Czern &amp; Coss.) varieties using SSR markers

Singh, K. H.; Singh, Lal; Parmar, Nehanjali; Sricharan, Kumar; Nanjundan, J.; Singh, Guman; Thakur, Ajay Kumar

doi:10.1371/journal.pone.0272914

Cited by 10 publications

(7 citation statements)

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“…Сучасні сорти ріпаку досить складно диференціювати, оскільки генетична база їх батьківських компонентів достатньо вузька. Наразі, за застосування традиційних методів аналізування відмінності сортів, таких як морфологічний опис, ізоферментний аналіз, складно виявити високий рівень поліморфізму, зокрема, враховуючи, що на ступінь прояву фенотипових ознак можуть також чинити вплив умови навколишнього середовища [5,6] [9]. Застосування SSR маркерів для оцінки полі-морфізму генотипів ріпаку є достатньо поширеним [10][11][12][13].…”

Section: український інститут експертизи сортів рослинunclassified

Determination of winter rapeseed (Brassica napus L.) polymorphism based on SSR markers and morphological characters

Piskova,

Kostenko,

Shliakhtun

et al. 2023

Agrobìologìâ

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The study presents the results of the genetic diversity estimation of winter rapeseed by molecular genetic analysis and the determination of polymorphism with morphological traits. The study aims to determine winter rapeseed hybrids polymorphism by SSR markers and the marker morphological characteristics. Twelve winter rapeseed hybrids which were examined within DUS testing and their 24 hereditary components were studied in 2021–2022. The study of rapeseed genotypes genetic diversity was carried out in 2021. It was determined that the majority of studied hybrids and their hereditary components by studied SSR markers are characterized with alleles of the same sizes and are homozygotic by these markers. Besides, it was found that the presence of only on one allele was identifed in hereditary components which was found in hybrids. This distribution allows to check the hybrid formula and to identify them. It was determined that the most polymorphic marker was Na12-A02, PIC is 0.77. The lowest value of PIC was obtained for Na12-E02 marker (0.47). On the average, for studied markers PIC is 0.66 which indicates the evenness of identifed alleles distribution by SSR markers among studied winter rapeseed genotypes. As results of cluster analysis, we obtained fve clusters of the studied hybrids by 8 SSR markers. The hybrids with genetic distances of 2.45 were the most similar hybrids. It was found that the hybrids with genetic distances of 5.83 and 5.74 were the most distinct. Three clusters were obtained as results of the cluster analysis based on morphological traits. It was determined that the most similar hybrids were the ones with genetic distances of 3.46. It was found that the most distinct hybrids had the genetic distances of 5.299.38. Thus, taking into account the various distribution of the studied genotypes by the SSR markers and morphological characteristics, SSR markers can be used as additional tool for the distinctness determination. Key words: genetic distances, winter rapeseed, allele frequencies, РІС, genetic diversity, SSR markers.

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Section: український інститут експертизи сортів рослинunclassified

Determination of winter rapeseed (Brassica napus L.) polymorphism based on SSR markers and morphological characters

Piskova,

Kostenko,

Shliakhtun

et al. 2023

Agrobìologìâ

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“…Bayesian clustering algorithms were performed in the STRUCTURE 2.3.4 software to describe the genetic structure better and to explain the results from previous statistical analyses. Although this approach has been highly frequented in ecological studies [38] in the last period, it is also used for agricultural crops including oil seed brassicas, such as B. napus [32], B. juncea [23], and B. carinata [25]. Through the STRUCTURE software, we determined the likelihood of the data for several different K, with K ranging from one to seven, to eventually see the plateau pattern in the diagnostic plot.…”

Section: Genetic Structure Of Yellow Mustard Varietiesmentioning

confidence: 99%

“…More attention was focused on coloured mustard, B. juncea, B. carinata, and B. nigra. An SSR marker was used in B. juncea [23] and B. nigra [24]. In B. juncea, the influence of natural outcrossing and cross-hybridization for the development of analysed varieties was demonstrated, based on the analysis of the genetic structure.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite Markers: A Tool to Assess the Genetic Diversity of Yellow Mustard (Sinapis alba L.)

Jozová,

Rost,

Rychlá

et al. 2023

Plants

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Microsatellite markers were used for the assessment of genetic diversity and genetic structure in a germplasm collection of yellow mustard, Sinapis alba L. The comprehensive collection of genetic resources represented 187 registered varieties, landraces, and breeding materials. Microsatellites generated 44 polymorphic alleles in 15 loci. Eleven of them were medium to highly polymorphic, and the high levels of observed heterozygosity (0.12–0.83) and Nei’s gene diversity index (0.11–0.68) indicated a high level of polymorphism. Based on PCoA and neighbor joining analyses, the genetic resources were divided into two groups. The range of genetic dissimilarity in the analysed collection was in the range of 0.00–1.00. The high level of dissimilarity between the accessions was documented by the high WAM value (33.82%). Bayesian clustering algorithms were performed in the STRUCTURE 2.3.4 software. The number of clusters was estimated at K = 2. The accessions were classified according to Q1/Q2 values. The low average values of the parameters Fst_1 (0.3482), Fst_2 (0.1916), and parameter alpha (0.0602) indicated substantial mating barriers between varieties and reproductive isolation due to the limited exchange of genetic resources between breeders. These results demonstrated the importance of extensive collections of genetic resources for the maintenance of genetic diversity and indicated considerable genetic differentiation among accessions.

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“…B. juncea is cultivated in India, China, Bangladesh, Pakistan, Australia, Canada and some European countries (Choudhury et al, 2023). It is predominantly cultivated as an oilseed crop in India over an estimated area of 6.2 million hectare, contributing to >85% of RM acreage (Singh et al, 2022). Indian mustard oil is widely consumed as an edible oil.…”

Section: Introductionmentioning

confidence: 99%

“…This is amounting to washing out a large exchequer of money (Sharma et al, 2022). It has been estimated that by 2025, the demand of edible oil in India will increase upto 34 million tonnes, and out of this, around 14 million tonnes had to be met by Indian mustard alone (Singh et al, 2022). Throughout the globe, various Brassica research groups are making concerted efforts for developing elite high-yielding varieties of B. juncea having resistance against biotic and abiotic stresses (Chauhan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Utilization of crop wild relatives for biotic and abiotic stress management in Indian mustard [Brassica juncea (L.) Czern. & Coss.]

Verma,

Dubey,

Singh

et al. 2023

Front. Plant Sci.

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Brassica juncea (L.) Czern. & Coss. (Indian mustard) is an economically important edible oil crop. Over the years, plant breeders have developed many elite varieties of B. juncea with better yield traits, but research work on the introgression of stress resilience traits has largely been lagging due to scarcity of resistant donors. Crop wild relatives (CWRs) are the weedy relatives of domesticated plant species which are left unutilized in their natural habitat due to the presence of certain undesirable alleles which hamper their yield potential, and thus, their further domestication. CWRs of B. juncea namely include Sinapis alba L. (White mustard), B. tournefortii Gouan. (African mustard), B. fruticulosa Cirillo (Twiggy turnip), Camelina sativa L. (Gold-of-pleasure), Diplotaxis tenuisiliqua Delile (Wall rocket), D. erucoides L. (White wall rocket), D. muralis L. (Annual wall rocket), Crambe abyssinica R.E.Fr. (Abyssinian mustard), Erucastrum gallicum Willd. (Common dogmustard), E. cardaminoides Webb ex Christ (Dogmustard), Capsella bursa-pastoris L. (Shepherds purse), Lepidium sativum L. (Garden Cress) etc. These CWRs have withstood several regimes of biotic and abiotic stresses over the past thousands of years which led them to accumulate many useful alleles contributing in resistance against various environmental stresses. Thus, CWRs could serve as resourceful gene pools for introgression of stress resilience traits into Indian mustard. This review summarizes research work on the introgression of resistance against Sclerotinia stem rot (caused by Sclerotinia sclerotiorum), Alternaria blight (caused by Alternaria brassicae), white rust (caused by Albugo candida), aphid attack, drought and high temperature from CWRs into B. juncea. However, various pre- and post-fertilization barriers due to different ploidy levels are major stumbling blocks in the success of such programmes, therefore, we also insightfully discuss how the advances made in -omics technology could be helpful in assisting various breeding programmes aiming at improvisation of stress resilience traits in B. juncea.

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Molecular characterization and genetic diversity analysis in Indian mustard (Brassica juncea L. Czern & Coss.) varieties using SSR markers

Cited by 10 publications

References 20 publications

Determination of winter rapeseed (Brassica napus L.) polymorphism based on SSR markers and morphological characters

Determination of winter rapeseed (Brassica napus L.) polymorphism based on SSR markers and morphological characters

Microsatellite Markers: A Tool to Assess the Genetic Diversity of Yellow Mustard (Sinapis alba L.)

Utilization of crop wild relatives for biotic and abiotic stress management in Indian mustard [Brassica juncea (L.) Czern. & Coss.]

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