Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers

Baig, M. N. R.; Grewal, Sapna; Dhillon, Santosh

doi:10.3906/tar-0804-27

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…In this study, we report relatively low genetic variability (43%, 42% and 48%) among species of C. virginica L. aestuans and S. rhombifolia in Lagos state. Limited genetic variability was reported by [32] among citrus cultivars similar to the findings of this work. The genetic diversity of plants is closely related to their geographic distribution (22).…”

Section: Resultssupporting

confidence: 90%

“…In the work of Srilekha and Ravi, [22], 100 percent polymorphism was observed in OPE-4 and OPA-9 primers similar to the finding of this research. Relatively high level of polymorphism were obtained in this study indicating moderate diversity between the genotypes similar to [32]. Dendrogram of similarity (Figure 1) constructed using Unweighted Pair Group of Method for arithmetic (UPGMA) with SM coefficient showed a genetic distance range of 0.57 to 1.00, 0.58 to 1.00 and 0.52 to 1.00 and clustered at 0.57, 0.58, 0.52 implying 57%, 58%, 52% similarity and 43%, 42% and 48% variability for C. virginica, L. aestuans and S. rhombifolia respectively.…”

Section: Resultsmentioning

confidence: 48%

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Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

E. Ani,

Ofodile,

Nwakanma

et al. 2023

Iraqi Journal of Science

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Laportea aestuans L. (Chew), Commelina virginica (L.) and Sida rhombifolia (L.) are common wild plants used in treating several ailments including diarrhea, dysentery, hernia, oedema, ulcers and many more in traditional African medicine especially, Nigeria. The potentials of Random Amplified polymorphic DNA (RAPD) primers in delimiting intra-specific variation in L. aestuans, C. virginica and S. rhombifolia was assessed using three RAPD primers. Plant and soil samples were collected from 19 local government areas in Lagos State and assessed for genetic and biochemical relationships. A total of 56 bands were produced of which 44 were polymorphic. Maximum number of bands (21) was produced by OPY20 while OPC04 produced minimum number of bands (14). The maximum and minimum percentage polymorphisms were 100% (OPC04) and 50% (OPA12), The dendrogram of genetic diversity had a genetic distance range of 0.57 to 1.00, 0.58 to 1.00 and 0.52 to 1.00 and clustered at 0.57, 0.58, 0.52 implying 57%, 58%, 52% similarity and 43%, 42% and 48% variability for L. aestuans, C. virginica and S. rhombifolia respectively. Results of phytochemical analysis show that S. rhombifolia is particularly high in steroids (I134.19±0.23), flavonoids (118.65±0.18), phenolics (125.25±1.43), alkaloids (63.56±0.11) and triterpenes (72.44±0.12) but low in saponins (23.29±0.05), glycosides (6.79±0.01) and tannins (7.10±0.02).

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 48%

Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

E. Ani,

Ofodile,

Nwakanma

et al. 2023

Iraqi Journal of Science

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“…These markers have been widely used for studying phylogenetic relationships in Citrus (Jung et al, 2005;Yingzhi et al, 2007;Jena et al, 2009;Li et al, 2010;Lu et al, 2011). Genetic diversity and phylogenetic relationships among many citrus cultivars have been analyzed using RAPD markers (Machado et al, 1996;Coletta-Filho et al, 1998;Federici et al, 1998;Abkenar and Isshiki, 2003;Baig et al, 2009) and SSR markers (Biswas et al, 2011;Shrestha et al, Phenotypic and Genetic Characterization of Three Different Types of Dangyooza (Citrus grandis), Korean Landrace Citrus 2012; Nematollahi et al, 2013;Ahmed et al, 2018). SSR markers have also been applied for citrus germplasm collection (Barkley et al, 2006) and identification of zygotic and nucellar seedling in citrus (Yildiz et al, 2013;Woo et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and Genetic Characterization of Three Different Types of Dangyooza (Citrus grandis), Korean Landrace Citrus

Yi¹,

Zhin²,

Oh³

et al. 2021

HST

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Dangyooza (DY), a Korean landrace citrus similar to pomelo (Citrus grandis L. Osbeck), has been consumed as a fruit and used in folk remedies to treat colds for centuries, and it is still used as a garden tree. DY has two natural variants, the buk-daengyooza (BDY) and the seol-daengyooza (SDY) based on distinguishable phenotypic fruit characteristics. However, there is little scientific analysis available not only on the morphological and chemical properties but also on the genetic basis of these natural variants. To gain a better understanding of DY, several morphological, phytochemical, chromosomal, and genetic traits were analyzed in DY and its natural variants, BDY and SDY. Morphological characteristics such as leaf and fruit shape, fruit hardness, and peel thickness were used to discriminate SDY from DY and BDY. Notably, SDY produced smaller fruit with thinner peels than those of DY and BDY. The major flavanones occurring in citrus were also markedly higher in SDY than those of DY and BDY. However, chromosomal configuration and genetic diversity analysis using random amplified polymorphic DNA, simple sequence repeat, and plastid trnL/F barcoding markers were unable to clearly discriminate the phylogenetic relationships among the DY types. This suggests that SDY might have arisen from somatic mutation, perhaps as a nucellar seedling.

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“…Table 3 shows the total number of enlarged and polymorphic fragments produced by each plant, as well as the distinct characteristics of each of the six species of mandarin and clementine (3). As a result, many fruit and fruit trees, especially cultivars, have been successfully used using RAPD tags (Baig et al, 2009, Leng et al, 2012.…”

Section: Rapd (Randomly Amplified Polymorphic Dna) Analysismentioning

confidence: 99%

RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars

Abdein

Imrahim²,

Mohamed

et al. 2022

Egyptian Journal of Horticulture

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T HE experiments were performed using (Nour, Fedela, Spinosa clementines, Kishu seedless, Balady, and Seedless mandarins) to determine the compatibility or incompatibility as related to the number of seeds per fruit and the genetic relation between them. For two consecutive seasons (2020 and 2021), the growth of the pollen tube in the styles following self-pollination has been analyzed using a fluorescence microscope to detect incompatibility properties. According to Nour and Fedela clementine cultivars are highly incompatible, while the other four cultivars are incompatible. After receiving pollen, Nour and Fedela clementines had very low pollen tubes that reached style. On the other hand, the highest rate was in the other four cultivars. The DNA of the six cultivars of mandarin and clementine investigated in this study was very similar. Balady mandarin and seedless mandarin appear to have very high similarities, while Fedela clementine and seedless mandarin appear to be very low. RAPD-PCR showed a specific molecule related to incompatibility such as (A-18) RAPD primer, at MW 361 bp. linked to the incompatibility of Nour and Fedela clementine c.vs.

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Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers

Cited by 10 publications

References 21 publications

Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

Phenotypic and Genetic Characterization of Three Different Types of Dangyooza (Citrus grandis), Korean Landrace Citrus

RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars

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