Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of Streptomyces coelicolor A3(2)

Abstract

Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics.

Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux

“…However, Streptomyces genomes still contain large numbers of functionally unknown TCS-coding genes [ 33 , 37 ]. Moreover, Streptomyces genomes also contain numerous HP genes with unknown functions [ 38 , 39 ]. For example, there are 3694 (47% of the total annotated genes) HP genes in S. coelicolor and 3604 (36% of the total genes) in S. bingchenggensis [ 39 , 40 ].…”

Designing a Recombinant Vaccine against Providencia rettgeri Using Immunoinformatics Approach

“…The proteins were regarded as good vaccine candidates only when the transmembrane helices were less than the threshold, as such proteins can be easily handled in experimental studies. The selected proteins were further processed in downward analysis [55].…”

Hypothetical protein predicted to be tumor suppressor: a protein functional analysis

“…With the advancement of Computational Biology, it has become easier to analyze hypothetical proteins using bioinformatics tools that provide various advantages like the determination of 3D structural conformation, identification of new domains and motifs, assessment of new cascades and pathways, phylogenetic profiling, and functional annotation [ 11 ]. A recent study showed that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to further research in bacterial genetics [ 12 ].…”