Molecular characterization and functional analysis of adrenergic like receptor during larval metamorphosis in Crassostrea angulata

Yang, Bingye; Qin, Jiaxuan; Shi, Bo; Han, Guodong; Huang, Heqing; Ke, Caihuan

doi:10.1016/j.aquaculture.2012.08.040

Cited by 25 publications

(11 citation statements)

References 32 publications

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“…These results suggested that CgGPR1 is also likely to play roles in adult organs. The expression pattern of CgGPR1 in the organs differed from those of other reported oyster biogenic amine receptors including the adrenergic receptor AR cga [48] and the DA receptor Ca-DA1R [26], which both exhibited highest expression levels in the labial palps. The different expression patterns between CgGPR1 and other tyrosine derivative receptors suggested the functional differentiation of GPCRs for different biogenic amine receptors in oysters.…”

Section: Resultsmentioning

confidence: 64%

Molecular Characterization and Functional Analysis of a Putative Octopamine/Tyramine Receptor during the Developmental Stages of the Pacific Oyster, Crassostrea gigas

Huang

et al. 2016

PLoS ONE

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Octopamine (OA) and its precursor, tyramine (TA), participate in invertebrate development such as growth, maturation, and reproduction by activating their corresponding G protein-coupled receptors (GPCRs). Although OA was first discovered in mollusks (octopus), subsequent studies on OA, TA and related receptors have primarily been conducted in Ecdysozoa, especially in insects. Accordingly, only limited reports on OA/TA receptors in mollusks are available and their physiological roles remain unclear. Here, a full-length cDNA encoding a putative 524 amino acid OA/TA receptor (CgGPR1) was isolated from the Pacific oyster Crassostrea gigas. CgGPR1 was most closely related to the Lymnaea stagnalis OA receptor OAR2 in sequence. Phylogenetic analysis showed that CgGPR1 belongs to a poorly studied subfamily of invertebrate OA/TA receptors. The spatio-temporal expression of CgGPR1 in C. gigas larvae was examined by quantitative real-time PCR and Western blot analysis. CgGPR1 was expressed during all developmental stages of C. gigas with higher levels at mid-developmental stages, indicating its potential role in embryogenesis and tissue differentiation. Immunoreactive fluorescence of CgGPR1 was mainly observed in the velum, foot, gill and mantle of C. gigas larvae. CgGPR1 transcripts were detected in all the tested organs of adult C. gigas, with highest level in the mantle. Pharmacological analysis showed that cAMP and Ca2+ concentrations remained unchanged in HEK293 cells expressing CgGPR1 upon addition of OA, TA or related amines, suggesting that CgGPR1 modulates other unknown molecules rather than cAMP and Ca2+. Our study sheds light on CgGPR1 function in oysters.

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Section: Resultsmentioning

confidence: 64%

Molecular Characterization and Functional Analysis of a Putative Octopamine/Tyramine Receptor during the Developmental Stages of the Pacific Oyster, Crassostrea gigas

Huang

et al. 2016

PLoS ONE

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“…In an effort to identify the specific receptor/s responsible for catecholamineinduced settlement and metamorphosis in bivalves, results of pharmacological and immunohistochem- characterized an adrenergic-like receptor gene (ARcga) in larvae of the oyster Crassostrea angulata (Lamark, 1819) and performed a temporal analysis of its expression during larval development through real-time qPCR and whole mount in situ hybridiza-395 tion. A clear peak in ARcga expression was observed during settlement and metamorphic competency and also after bath application of whole larvae in epinephrine (Yang et al 2012). In a subsequent investigation to identify downstream 400 components of the epinephrine-induced pathway, also provided evidence for the involvement of Receptor for Activated C Kinase 1 (RACK1) during larval metamorphosis of C. angulata and demonstrated that its gene and protein expressions were up-regulated by epinephrine treatment.…”

Section: Discussionmentioning

confidence: 92%

Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

Young

Sánchez-Lazo

Robertson

2015

Marine Biology Research

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Settlement responses were investigated for mussel (Perna canaliculus) larvae after exposure to catecholamines and their precursor metabolites. Settlement and mortality assays were conducted in Petri plates with chemical treatments (L-phenylalanine, L-tyrosine, L-DOPA, dopamine hydrochloride and epinephrine at various concentrations) and controls. 15The proteinogenic amino acids L-phenylalanine and L-tyrosine both were effective inducers (~65%) of larval settlement at 10 −5 mol L −1 compared with controls (4%). Exposure of larvae to L-DOPA, dopamine and epinephrine resulted in maximum settlement induction (50, 60 and 51%, respectively) at 10 −5 mol L −1 . Larval mortalities were low (comparable to controls) across all concentrations of L-phenylalanine and L-tyrosine treatments whereas high mortalities (>60%) were observed for L-DOPA, dopamine and epinephrine at concentrations ≥ 10 −4 mol L −1. Our results indicate that P. canaliculus 20 larval settlement is under endogenous regulation by a catecholaminergic mechanism. Furthermore, the inductive effects of all tested metabolites in the epinephrine biosynthesis pathway point to a putative involvement of adrenergic-type receptors in regulation of larval settlement in this mussel species.

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“…The samples were washed with 1×PBS (phosphate-buffered saline), frozen in liquid nitrogen and stored at -80°C until processed. Larvae culture of C. angulata was conducted as previously described [ 20 ]. For whole mount in-situ hybridization (WMISH), trochophore were collected and then fixed directly in 4% paraformaldehyde overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…The quantity of RNA was determined by measuring OD 260nm with the NanoDrop ND-1000 UV-Visible Spectrophotometer (ThermoScientific, USA). The total RNA was reverse-transcribed into cDNA as described previously [ 20 ]. The first strand of the cDNA was synthesized and used as the template for further PCR analysis.…”

Section: Methodsmentioning

confidence: 99%

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Molecular Analysis of Atypical Family 18 Chitinase from Fujian Oyster Crassostrea angulata and Its Physiological Role in the Digestive System

Yang

Zhang

et al. 2015

PLoS ONE

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Chitinolytic enzymes have an important physiological significance in immune and digestive systems in plants and animals, but chitinase has not been identified as having a role in the digestive system in molluscan. In our study, a novel chitinase homologue, named Ca-Chit, has been cloned and characterized as the oyster Crassostrea angulate. The 3998bp full-length cDNA of Ca-Chit consisted of 23bp 5-UTR, 3288 ORF and 688bp 3-UTR. The deduced amino acids sequence shares homologue with the chitinase of family 18. The molecular weight of the protein was predicted to be 119.389 kDa, with a pI of 6.74. The Ca-Chit protein was a modular enzyme composed of a glycosyl hydrolase family 18 domain, threonine-rich region profile and a putative membrane anchor domain. Gene expression profiles monitored by quantitative RT-PCR in different adult tissues showed that the mRNA of Ca-Chit expressed markedly higher visceral mass than any other tissues. The results of the whole mount in-situ hybridization displayed that Ca-Chit starts to express the visceral mass of D-veliger larvae and then the digestive gland forms a crystalline structure during larval development. Furthermore, the adult oysters challenged by starvation indicated that the Ca-Chit expression would be regulated by feed. All the observations made suggest that Ca-Chit plays an important role in the digestive system of the oyster, Crassostrea angulate.

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Molecular characterization and functional analysis of adrenergic like receptor during larval metamorphosis in Crassostrea angulata

Cited by 25 publications

References 32 publications

Molecular Characterization and Functional Analysis of a Putative Octopamine/Tyramine Receptor during the Developmental Stages of the Pacific Oyster, Crassostrea gigas

Molecular Characterization and Functional Analysis of a Putative Octopamine/Tyramine Receptor during the Developmental Stages of the Pacific Oyster, Crassostrea gigas

Putative involvement of adrenergic receptors in regulation of mussel (Perna canaliculus) larval settlement

Molecular Analysis of Atypical Family 18 Chitinase from Fujian Oyster Crassostrea angulata and Its Physiological Role in the Digestive System

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