Molecular characterization and functional analysis of the vitellogenin receptor in the rice stem borer, <i>Chilo suppressalis</i>

Miao, Lijun; Zhang, Nan; Jiang, Heng; Fan, Daiming; Yang, Xuemei; Qian, Kun; Meng, Xiangkun; Wang, Jianjun

doi:10.1002/arch.21636

Cited by 6 publications

(9 citation statements)

References 34 publications

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“…However, intensive use of insecticides has driven C. suppressalis to develop resistance to a wide range of insecticides (Su et al, 2014;Lu et al, 2017;Yao et al, 2017). Hence, the development of RNA interference (RNAi)mediated disruption of reproduction represents an alternative control strategy of C. suppressalis (Miao et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The reproductive success of insects depends on vitellogenin (Vg) biosynthesis and uptake of Vg into developing oocytes mediated by vitellogenin receptor (VgR). Recently, we have characterized the C. suppressalis CsVg and CsVgR at the molecular levels (Huang et al, 2016;Miao et al, 2020). In this study, full-length cDNAs of two paralogous Met genes (named as CsMet1 and CsMet2) were isolated from C. suppressalis.…”

Section: Introductionmentioning

confidence: 99%

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Involvement of Two Paralogous Methoprene-Tolerant Genes in the Regulation of Vitellogenin and Vitellogenin Receptor Expression in the Rice Stem Borer, Chilo suppressalis

et al. 2020

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Besides the function of preventing metamorphosis in insects, the juvenile hormone (JH) plays a role in female reproduction; however, the underlying mechanism is largely unknown. The methoprene-tolerant (Met) protein belongs to a family of basic helix-loophelix-Per-Arnt-Sim (bHLH-PAS) transcription factors and functions as the JH intracellular receptor. In this study, two full length cDNAs encoding Met (CsMet1 and CsMet2) were isolated from the rice stem borer, Chilo suppressalis. Structural analysis revealed that both CsMet1 and CsMet2 exhibited typical bHLH, PAS-A, PAS-B, and PAC (PAS C terminal motif) domains. Comparative analysis of transcript level using reverse transcription-quantitative PCR (RT-qPCR) revealed that CsMet1 was predominant in almost all examined developmental stages and tissues. Treatment with methoprene in vivo induces the transcription of both CsMet1 and CsMet2. Notably, injection of dsCsMet1 and dsCsMet2 suppressed the expression levels of vitellogenin (CsVg) and Vg receptor (CsVgR). These findings revealed the potential JH signaling mechanism regulating C. suppressalis reproduction, and provided evidence that RNAi-mediated knockdown of Met holds great potential as a control strategy of C. suppressalis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Involvement of Two Paralogous Methoprene-Tolerant Genes in the Regulation of Vitellogenin and Vitellogenin Receptor Expression in the Rice Stem Borer, Chilo suppressalis

et al. 2020

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“…VgR belongs to low‐density lipoprotein receptor superfamily (Raikhel & Dhadialla, 1992; Sappington & Raikhel, 1998), and plays indispensible role in vitellogenesis (Cong et al, 2015; Shang et al, 2018). Knockdown VgR by RNAi or knockout VgR by Crispr/cas9 in many insects produces similar phenotypes such as reduced Vgs in ovary, accumulation of Vgs in hemolymph and reduced fecundity (Du et al, 2019; Hu et al, 2019; Husain et al, 2020; Liu et al, 2019; Lu et al, 2015; Miao et al, 2020; Mitchell et al, 2007; Peng et al, 2019; Shang et al, 2018; Shu et al, 2016; Thomas et al, 2020; Tufail & Takeda, 2005; Xiang et al, 2019), suggesting a conserved role for VgR in insects. One VgR exists in locust and is expressed primarily in ovary (Ferenz, 1993).…”

Section: Discussionmentioning

confidence: 99%

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Wang

Deng

et al. 2021

Arch Insect Biochem Physiol

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DDX3 represents a well‐defined subfamily of DEAD‐box RNA helicase and exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility. Our previous study has shown that LmDDX3, the ortholog of DDX3 in Locusta migratoria, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown of LmDDX3 results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. In this study, we therefore characterized LmDDX3 expression in female adult locust and analyzed its function in oocyte development. LmDDX3 was expressed in all tissues examined with significant more transcripts in ovary and hindgut. In ovary, a strong expression level was detected at the day just after adult eclosion, and a dramatic reduction then occurred during the oocyte development. LmDDX3 RNAi led to a reduced vitellogenin (Vg) expression in fat body via partially at least, the JH signaling pathway, and caused an upregulation of vitellogenin receptor (VgR) in ovary, and thus blocked the ovarian development and oocyte maturation. Sequence and phylogenetic analysis indicated that LmDDX3 was closely related to termite DDX3. Taken together, these data reveal a critical role for LmDDX3 in regulating the transcription of Vg and VgR, two major factors in vitellogenesis that is a key process required for ovary development and oocyte maturation in locust, and contribute thereof a new putative target for locust biological control.

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“…There is only one single VgR gene in most insects, encoding a large protein of 180–214 kDa [ 3 , 5 ]. VgRs are members of the low-density lipoprotein receptor (LDLR) family, which contains five typical domains: a ligand-binding domain (LBD), an epidermal growth factor precursor domain (EGF) with low-density lipoprotein-receptor Tyr-Trp-Thr-Asp (YWTD) repeats, an O-linked sugar domain, a transmembrane domain (TMD), and a cytoplasmic tail (CD) [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Vitellogenin and Its Receptor in Spodoptera frugiperda (J. E. Smith, 1797), and Their Function in Reproduction of Female

Han

Wang

Song

et al. 2022

IJMS

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The fall armyworm Spodoptera frugiperda is a highly polyphagous invasive pest. The strong reproductive capacity is an important factor in the rapid colonization and expansion of S. frugiperda. Vitellogenin (Vg) and vitellogenin receptor (VgR) play important roles in insect reproduction. As the precursor of vitellin (Vn), Vg provides essential nutrition for embryonic development, and VgR mediates the uptake of Vg by oocytes. In this context, we cloned and characterized these two genes of S. frugiperda (SfVg and SfVgR) and evaluated their expression profiles in different developmental stages and tissues. The RNA interference experiment was used to investigate their function in vitellogenesis. The ORF values of SfVg and SfVgR were 5250 and 5445 bp, encoding 1749 and 1815 amino acid residues, respectively. The qRT-PCR results revealed that both SfVg and SfVgR were highly expressed in female adults; SfVg was specifically expressed in the fat body, whereas SfVgR was highly expressed in the ovary. In addition, the depletion of either SfVg or SfVgR hindered oocyte maturation and ovarian development, leading to a significant decrease in fecundity. The present study reveals the importance of SfVg and SfVgR in the vitellogenesis of S. frugiperda, laying a theoretical foundation for the development of pollution-free pest control strategies with SfVg and SfVgR as new targets.

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Molecular characterization and functional analysis of the vitellogenin receptor in the rice stem borer, Chilo suppressalis

Cited by 6 publications

References 34 publications

Involvement of Two Paralogous Methoprene-Tolerant Genes in the Regulation of Vitellogenin and Vitellogenin Receptor Expression in the Rice Stem Borer, Chilo suppressalis

Involvement of Two Paralogous Methoprene-Tolerant Genes in the Regulation of Vitellogenin and Vitellogenin Receptor Expression in the Rice Stem Borer, Chilo suppressalis

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Molecular Characterization of Vitellogenin and Its Receptor in Spodoptera frugiperda (J. E. Smith, 1797), and Their Function in Reproduction of Female

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