Molecular characterization and functional analysis of CzR1, a coiled-coil-nucleotide-binding-site-leucine-rich repeat R-gene from Curcuma zedoaria Loeb. that confers resistance to Pythium aphanidermatum

Molecular cloning of a coiled-coil-nucleotide-binding-site-leucine-rich repeat gene from pearl millet and its expression pattern in response to the downy mildew pathogen

“…Our studies on the quantification of RGA RGPM 301 by qRT-PCR exhibited low level expression of transcript in the pearl millet highly resistant (IP18296) and highly susceptible (7042S) cultivars without pathogen challenge. The CzR1 of Curcuma zedoaria also revealed low transcript level prior to Pythium aphanidermatum infection which is in concurrence with our results [34]. Further, inoculation with downy mildew pathogen S. graminicola significantly up-regulated the transcript levels of RGA RGPM 301 in the resistant pearl millet cultivar.…”

Molecular characterization of Fom-1 gene and development of functional markers for molecular breeding of resistance to Fusarium race 2 in melon

“…This result is consistent with the findings of Wang et al (2014) who showed, through quantitative real time PCR, low level of Fom-2 transcript in the un-inoculated plants. A similar pattern has been observed in a majority of cloned R-genes (Mes et al 2000;Song et al 2003;Tan et al 2007;Kar et al 2013). Moreover, RT-PCR revealed specific transcript expression of Fom-1 gene either in resistant and susceptible material after Fom infection.…”

Analysis of structure and differential expression of Pseudomonas syringae 5-like(RPS5-like) genes in pathogen-infected Vitis flexuosa

“…Kortekamp et al (2008) also reported that the CC-NBS-LRR family gene, VRP1, was induced during pathogen attack in the resistant grapevine cultivar 'Gloire de Montpellier' . It has been reported that CzR1, a CC-NBS-LRR R-gene found in turmeric plants, conferred resistance against Pythium aphanidermatum via upregulated expression under pathogen challenge (Kar et al, 2013). Similarly, transcripts of powdery mildew resistance genes, Mla6 and Mla13, were induced after inoculation of Blumeria graminis f.sp.…”

“…This ATP-bound form of RPS5 engages in downstream signaling molecules that activate the defense response (Ade et al, 2007;Qi et al, 2012). More than 50 NBS-LRR R-genes, including the almost 40 CC-NBS-LRR, conferred resistance against several pathogens in plants such as Arabidopsis, tomato, potato, barley, rice, maize, apple, grape, lettuce, pepper, sunflower, melon, and soybean (Kortekamp et al, 2008;Joshi and Nayak, 2011;Kar et al, 2013;Arya et al, 2014).…”