Molecular characterization and expressional affirmation of the beta proteasome subunit cluster in rock bream immune defense

Abstract

Immunoproteasomes are primarily induced upon infection and formed by replacing constitutive beta subunits with inducible beta subunits which possess specific cleavage properties that aid in the release of peptides necessary for MHC class I antigen presentation. In this study, we report the molecular characterization and expression analysis of the inducible immunosubunits PSMB8, PSMB9, PSMB9-L, and PSMB10 from rock bream, Oplegnathus fasciatus. The three subunits shared common active site residues and were placed in close proximity to fish homologues in the reconstructed phylogenetic tree, in which the mammalian homologues formed separate clades, indicating a common ancestral origin. The rock bream immunosubunits possessed higher identity and similarity with the fish homologues. RbPSMB8, RbPSMB9, RbPSMB9-L, and RbPSMB10 were multi-exonic genes with 6, 6, 7 and 8 exons, respectively. These four genes were constitutively expressed in all the examined tissues. Immunostimulants such as lipopolysaccharide and poly I:C induced RbPSMB8, RbPSMB9, RbPSMB9-L, and RbPSMB10 in liver and head kidney, suggesting their possible involvement in immune defense in rock bream.

Overgrazing induces alterations in the hepatic proteome of sheep (Ovis aries): an iTRAQ-based quantitative proteomic analysis

“…The up-regulation of TMBIM6 and CCAR2 indicates that overgrazing may lead to a long-term stressful situation and trigger apoptosis in sheep hepatocytes. Other proteins involved in the immune response, including NT5E (5’-nucleotidase, ecto), PPM1B (protein phosphatase, Mg 2+ /Mn 2+ dependent, 1B), PSMB10 (proteasome subunit beta 1) and TRIM56 (tripartite motif containing 56), are down-regulated, which suggests that the sheep immunity is suppressed during overgrazing, thus increasing the likelihood of bacterial or viral infection and reduced growth performance [ 41 , 42 ]. These observations were consistent with the reduced immune organ indexes and serum levels of immunoglobulins and the increased concentrations of inflammatory cytokines in OG sheep.…”

Network-based integration of omics, physiological and environmental data in real-world Elbe estuarine Zander

“…So far there is relatively little research focusing in depth on proteasomal gene expression in sh. Enhanced expression of psmb (8, 9, 9-L, 10) was shown during antibacterial and antiviral response [133] used as discriminative markers for infection monitoring [134].…”

“…So far there is relatively little research focusing in depth on proteasomal gene expression in sh. Enhanced expression of psmb (8, 9, 9-L, 10) was shown during antibacterial and antiviral response [133] used as discriminative markers for infection monitoring [134]. The expression and high intramodular connectivity of a full set of 20S proteasome, proteasome activator and inducible immunoproteasome indicate elevation of oxidized proteins due to oxygen stress and elevated ROS-levels and increased activity of immunologically active cells in the gill tissue of Hamburg harbor sh coordinated by irf1b signaling.…”

Major Histocompatibility Complex (MHC) Genes and Disease Resistance in Fish

“…PSMB12 (previously also named LMP2-like, LMP2/δ, PSMB9L or PSMB9B) and PSMB13 (previously also named PSMB7 or PSMB10) genes are members of the PSMB6/9/12 and PSMB7/10/13 families, respectively, found in teleost fish, and their levels of divergence from the respective other two family members suggest that they may be ancient [51,54,56,57]. The implication of these teleost genes in immune responses was not only suggested by their linkage within the Mhc region, but also by the increased expression of teleost PSMB8 , PSMB9 , PSMB10 (named PSMB7 in [162]), PSMB12 , PSMB13 , TAP2 and tapasin after immune stimulation (e.g., [162,163,164,165]). In humans, the molecules involved in the classical MHC class I peptide loading pathway show little allelic variation (e.g., [54]), but in rat allelic variations in TAP2 are found which appear to affect the peptides that can be presented by classical MHC class I [166,167,168] and a similar situation is found for TAP1, TAP2 and tapasin in chicken [169,170,171].…”