Molecular characterization and expression profiles of neuropeptide precursors in the migratory locust

Li, Hou; Jiang, Feng; Yang, Pengcheng; Wang, Xianhui; Kang, Le

doi:10.1016/j.ibmb.2015.05.014

Cited by 27 publications

(29 citation statements)

References 56 publications

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“…We have previously shown that 15 neuropeptide-encoding genes are differentially expressed in the brains of G-phase and S-phase locusts (Hou et al, 2015). Here, we extend our work to explore which of these neuropeptides are closely tied to the behavioral phase transition.…”

Section: Resultsmentioning

confidence: 99%

“…The migratory locust genome contains two NPF1 precursors (NPF1a and NPF1b) and an NPF2 precursor. NPF1b , which encodes an 86 aa peptide, is hardly detected in the locust brain because of its extremely low expression level (Hou et al, 2015). ( B ) Phylogenetic relationship between insect NPF/Y receptors and human NPY recepetors.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we have found that several neuropeptide genes are differentially expressed between the central nervous systems of G-phase and S-phase locusts (Hou et al, 2015), suggesting possible modulatory roles for these neuropeptides in the behavioral phase transition.…”

Section: Introductionmentioning

confidence: 99%

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The neuropeptide F/nitric oxide pathway is essential for shaping locomotor plasticity underlying locust phase transition

Yang

Jiang

et al. 2017

eLife

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Behavioral plasticity is widespread in swarming animals, but little is known about its underlying neural and molecular mechanisms. Here, we report that a neuropeptide F (NPF)/nitric oxide (NO) pathway plays a critical role in the locomotor plasticity of swarming migratory locusts. The transcripts encoding two related neuropeptides, NPF1a and NPF2, show reduced levels during crowding, and the transcript levels of NPF1a and NPF2 receptors significantly increase during locust isolation. Both NPF1a and NPF2 have suppressive effects on phase-related locomotor activity. A key downstream mediator for both NPFs is nitric oxide synthase (NOS), which regulates phase-related locomotor activity by controlling NO synthesis in the locust brain. Mechanistically, NPF1a and NPF2 modify NOS activity by separately suppressing its phosphorylation and by lowering its transcript level, effects that are mediated by their respective receptors. Our results uncover a hierarchical neurochemical mechanism underlying behavioral plasticity in the swarming locust and provide insights into the NPF/NO axis.DOI: http://dx.doi.org/10.7554/eLife.22526.001

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The neuropeptide F/nitric oxide pathway is essential for shaping locomotor plasticity underlying locust phase transition

Yang

Jiang

et al. 2017

eLife

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“…5 ). AstC, NPF, OK, and TK are conserved brain-gut peptides, and their functions include myotropic effects and regulation of feeding behavior 56 . Our findings indicate that they may play a significant role in the digestive system.…”

Section: Resultsmentioning

confidence: 99%

Identification and expression profiles of neuropeptides and their G protein-coupled receptors in the rice stem borer Chilo suppressalis

Teng

et al. 2016

Sci Rep

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In insects, neuropeptides play important roles in the regulation of multiple physiological processes by binding to their corresponding receptors, which are primarily G protein-coupled receptors (GPCRs). The genes encoding neuropeptides and their associated GPCRs in the rice stem borer Chilo suppressalis were identified by a transcriptomic analysis and were used to identify potential targets for the disruption of physiological processes and the protection of crops. Forty-three candidate genes were found to encode the neuropeptide precursors for all known insect neuropeptides except for arginine-vasopressin-like peptide (AVLP), CNMamide, neuropeptide-like precursors 2-4 (NPLP2-4), and proctolin. In addition, novel alternative splicing variants of three neuropeptide genes (allatostatin CC, CCHamide 1, and short neuropeptide F) are reported for the first time, and 51 putative neuropeptide GPCRs were identified. Phylogenetic analyses demonstrated that 44 of these GPCRs belong to the A-family (or rhodopsin-like), 5 belong to the B-family (or secretin-like), and 2 are leucine-rich repeat-containing GPCRs. These GPCRs and their likely ligands were also described. qRT-PCR analyses revealed the expression profiles of the neuropeptide precursors and GPCR genes in various tissues of C. suppressalis. Our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in Lepidoptera and aid in the design of peptidomimetics, pseudopeptides or small molecules capable of disrupting the physiological processes regulated by these signaling molecules and their receptors.

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“…Neuropeptides are usually produced from the cleavage of larger precursors and are usually modified post-transcriptionally to form isopeptides, which are then transported to target cells to activate corresponding receptors (Veenstra, 2000;Pauls et al, 2014;Yeoh et al, 2017). A large number of neuropeptides and their receptors have been extensively characterized and functionally validated in various insects, such as Drosophila melanogaster (Nässel and Winther, 2010), Tribolium castaneum (Li et al, 2007;Hauser et al, 2008), Locusta migratoria (Veenstra, 2014;Hou et al, 2015), and Bombyx mori (Roller et al, 2008). These results suggest significant neuropeptide and receptor variation between different orders, even between different species from the same order (Veenstra, 2019).…”

Section: Introductionmentioning

confidence: 99%

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

et al. 2020

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The red palm weevil Rhynchophorus ferrugineus is a devastating, invasive pest that causes serious damages to palm trees, and its invasiveness depends on its strong ability of physiological and behavioral adaptability. Neuropeptides and their receptors regulate physiology and behavior of insects, but these protein partners have not been identified from many insects. Here, we systematically identified neuropeptide precursors and the corresponding receptors in the red palm weevil, and analyzed their tissue expression patterns under control conditions and after pathogen infection. A total of 43 putative neuropeptide precursors were identified, including an extra myosuppressin peptide was identified with amino acid substitutions at two conserved sites. Forty-four putative neuropeptide receptors belonging to three classes were also identified, in which neuropeptide F receptors and insulin receptors were expanded compared to those in other insects. Based on qRT-PCR analyses, genes coding for several neuropeptide precursors and receptors were highly expressed in tissues other than the nervous system, suggesting that these neuropeptides and receptors play other roles in addition to neuro-reception. Some of the neuropeptides and receptors, like the tachykininrelated peptide and receptor, were significantly induced by pathogen infection, especially sensitive to Bacillus thuringiensis and Metarhizium anisopliae. Systemic identification and initial characterization of neuropeptides and their receptors in the red palm weevil provide a framework for further studies to reveal the functions of these ligand-and receptor-couples in regulating physiology, behavior, and immunity in this important insect pest species.

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Molecular characterization and expression profiles of neuropeptide precursors in the migratory locust

Cited by 27 publications

References 56 publications

The neuropeptide F/nitric oxide pathway is essential for shaping locomotor plasticity underlying locust phase transition

The neuropeptide F/nitric oxide pathway is essential for shaping locomotor plasticity underlying locust phase transition

Identification and expression profiles of neuropeptides and their G protein-coupled receptors in the rice stem borer Chilo suppressalis

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

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