Molecular Characterization and Expression Patterns of Two Pheromone-Binding Proteins from the Diurnal Moth Phauda flammans (Walker) (Lepidoptera: Zygaenoidea: Phaudidae)

Abstract

Sex pheromone-binding proteins (PBPs) play an important role in sex pheromone recognition in Lepidoptera. However, the mechanisms of chemical communication mediating the response to sex pheromones remain unclear in the diurnal moths of the superfamily Zygaenoidea. In this study, Phauda flammans (Walker) (Lepidoptera: Zygaenoidea: Phaudidae) was used as a model insect to explore the molecular mechanism of sex pheromone perception in the superfamily Zygaenoidea. Two novel pheromone-binding proteins (PflaPBP1 and PflaPBP2) from P. flammans were identified. The two pheromone-binding proteins were predominantly expressed in the antennae of P. flammans male and female moths, in which PflaPBP1 had stronger binding affinity to the female sex pheromones Z-9-hexadecenal and (Z, Z, Z)-9, 12, 15-octadecatrienal, PflaPBP2 had stronger binding affinity only for (Z, Z, Z)-9, 12, 15-octadecatrienal, and no apparent binding affinity to Z-9-hexadecenal. The molecular docking results indicated that Ile 170 and Leu 169 are predicted to be important in the binding of the sex pheromone to PflaPBP1 and PflaPBP2. We concluded that PflaPBP1 and PflaPBP2 may be responsible for the recognition of two sex pheromone components and may function differently in female and male P. flammans. These results provide a foundation for the development of pest control by exploring sex pheromone blocking agents and the application of sex pheromones and their analogs for insect pests in the superfamily Zygaenoidea.

Molecular mechanism of sex pheromone perception in male Mythimna loreyi revealed by in vitro system

“…In vitro and in vivo functional studies confirmed that PBP1 plays the major role and PBP2 and PBP3 are less important in female sex pheromone perception 9,14–17 . In addition to pheromone transportation, PBPs are also suggested to have roles in recognition among pheromone components, 18–21 and enhancement of the OR's sensitivity 22 …”

“…13 In vitro and in vivo functional studies confirmed that PBP1 plays the major role and PBP2 and PBP3 are less important in female sex pheromone perception. 9,[14][15][16][17] In addition to pheromone transportation, PBPs are also suggested to have roles in recognition among pheromone components, [18][19][20][21] and enhancement of the OR's sensitivity. 22 PRs refer to an OR subfamily that primarily function to perceive sex pheromone components, playing a central role in determining specificity of ORNs.…”