Molecular Characterization and Expression Pattern of Rheb Gene in Inner Mongolia Cashmere Goat (Capra hircus)

Xu, Zheng; Yang, Jing; Wang, Xiaojing; Liang, Yan; Wu, Manlin; Shi, Jiejun; Zhang, Tao; Yin, Qin; Li, Shuyu; Hao, Xiang; Wang, Zhigang; Liu, Dongjun

doi:10.1016/s1671-2927(11)60138-7

Cited by 6 publications

(7 citation statements)

References 30 publications

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“…The products of cashmere goat Rheb gene and FKBP38 gene were characterized by informatics in our previous study. Goat Rheb has a RAS domain (consisting of switch I and switch II domains) from residues 4 to 170 and a carboxyl-terminal CAAX box ( Zheng et al, 2011 ). Goat FKBP38 contains an FKBP_C domain, 2 TPR domains, and a TM domain and CAAX box ( Zheng et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…Rheb interacts directly with mTOR and activates mTOR kinase ( Yadav et al, 2013 ), whereas Rabin8 interacts with Rheb and inhibits the phosphorylation of Ser235/Ser236 in small ribosomal subunit protein S6 ( Parkhitko et al, 2011 ). Rheb is also regulated through direct phosphorylation by MAP kinase-activated protein kinase 5, and Rheb-mediated mTORC1 activation is inhibited ( Zheng et al, 2011 ); cyclic adenosine monophosphate can control mTOR activation by regulating the dynamic interaction between Rheb and phosphodiesterase 4D (Kim et al, 2011), and the N-methyl-D-aspartate receptor subunit N-methyl-D-aspartate 3A can interact with Rheb to function as a break of mTOR-dependent synaptic translation ( Sucher et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…pMD19T-Rheb and pMD19T-FKBP38 were generated in our previous work ( Zheng et al, 2011 ; 2012 ). The two plasmids were digested with restriction enzymes, respectively (TaKaRa, Dalian, China), to yield the Rheb and FKBP38 genes.…”

Section: Methodsmentioning

confidence: 99%

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Direct Interaction between Ras Homolog Enriched in Brain and FK506 Binding Protein 38 in Cashmere Goat Fetal Fibroblast Cells

Wang

Wang²,

et al. 2014

Asian Australas. J. Anim. Sci

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Ras homolog enriched in brain (Rheb) and FK506 binding protein 38 (FKBP38) are two important regulatory proteins in the mammalian target of rapamycin (mTOR) pathway. There are contradictory data on the interaction between Rheb and FKBP38 in human cells, but this association has not been examined in cashmere goat cells. To investigate the interaction between Rheb and FKBP38, we overexpressed goat Rheb and FKBP38 in goat fetal fibroblasts, extracted whole proteins, and performed coimmunoprecipitation to detect them by western blot. We found Rheb binds directly to FKBP38. Then, we constructed bait vectors (pGBKT7-Rheb/FKBP38) and prey vectors (pGADT7-Rheb/FKBP38), and examined their interaction by yeast two-hybrid assay. Their direct interaction was observed, regardless of which plasmid served as the prey or bait vector. These results indicate that the 2 proteins interact directly in vivo. Novel evidence is presented on the mTOR signal pathway in Cashmere goat cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Direct Interaction between Ras Homolog Enriched in Brain and FK506 Binding Protein 38 in Cashmere Goat Fetal Fibroblast Cells

Wang

Wang²,

et al. 2014

Asian Australas. J. Anim. Sci

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“…In this study, leucine lies at the 12 th position and the arginine residue lies at the 15 th position. The replacement also similarly occurs in the deduced goat Rheb amino acid sequence (Zheng et al, 2011).…”

Section: Bioinformatics Analysismentioning

confidence: 62%

“…Previous studies revealed that rat Rheb gene is expressed at comparatively high levels in the cortex of the brain as well as a number of peripheral tissues (Yamagata et al, 1994), while human Rheb2 is ubiquitously expressed, with the highest levels of transcript observed in skeletal and cardiac muscle, not in brain (Gromov et al, 1995). The semi-quantitative RT-PCR analysis of Rheb gene expression patterns in the Inner Mongolia Cashmere Goat (Capra hircus) indicated that Rheb gene is expressed in seven tested tissues and that the highest level of mRNA accumulation was detected in brains (Zheng et al, 2011). All of these results suggested species-specific expression of the Rheb gene despite its conservation of molecular characteristics during evolution.…”

Section: Spatial Transcript Profile Of the Rongchang Piglet Rheb Genementioning

confidence: 99%

Molecular characteristics and expression profile of the Rheb gene in Rongchang piglets (Sus scrofa)

Gan

Mei

Yang

et al. 2015

IJAR

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To give some insights into the genetic functions of Rheb signaling pathway in Rongchang piglets, this study reported the identification of complete coding sequence of the Rheb gene in Rongchang piglets and indicated that the complete coding region sequence is 555bp in length and encodes 184 amino acids, containing nearly all the conserved protein domains in the Rheb protein family. The deduced amino acid sequence identity between the Rongchang pig and sheep, cattle, or goat is 100%, 99% and 98%, respectively. Spatial transcriptional profile analysis indicated that the Rheb gene is expressed at remarkably high levels in both salivary gland and brain, especially in hypothalamus and ventral hippocampus (VH) regions of brain. Developmental expression profile analysis revealed the expression pattern is different between brain sub-regions and salivary gland of these two breeds of piglets, with the similar expression pattern despite various mRNA levels between Rongchang and Large White piglets.

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Proteasome subunit beta type 1 interacts directly with Rheb and regulates the cell cycle in Cashmere goat fetal fibroblasts

Wang

Guo

Liu

et al. 2017

Animal Cells and Systems

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PSMB1 (proteasome subunit beta type 1) is a core component of the 20S proteasome, and based on its structure, it might have a crucial function in the transcription of certain genes. Rheb (Ras homolog enriched in brain) is a Ras-like small GTPase that acts as an upstream positive regulator of the mechanistic target of rapamycin complex 1 (mTORC1) pathway. We cloned and characterized PSMB1 (KY310590.1) to determine its function in cell cycle progression and proliferation of goat fetal fibroblasts (GFbs). Further, by yeast two-hybrid screen and coimmunoprecipitation, we conformed that PSMB1 interacts directly with Rheb. An siRNA was designed and expressed targeting PSMB1 mRNA in GFbs and inducing cell cycle arrest. Rheb overexpression in GFbs significantly increased the number of S phase cells and growth efficiency compared with control cells. These data indicate that PSMB1 and Rheb have important functions in the cell cycle and proliferation of GFbs, indicating that their interaction governs many processes in GFbs.

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Molecular Characterization and Expression Pattern of Rheb Gene in Inner Mongolia Cashmere Goat (Capra hircus)

Cited by 6 publications

References 30 publications

Direct Interaction between Ras Homolog Enriched in Brain and FK506 Binding Protein 38 in Cashmere Goat Fetal Fibroblast Cells

Direct Interaction between Ras Homolog Enriched in Brain and FK506 Binding Protein 38 in Cashmere Goat Fetal Fibroblast Cells

Molecular characteristics and expression profile of the Rheb gene in Rongchang piglets (Sus scrofa)

Proteasome subunit beta type 1 interacts directly with Rheb and regulates the cell cycle in Cashmere goat fetal fibroblasts

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