Molecular Characterization and Expression of two Ovarian Lipoprotein Receptors in the Rainbow Trout, Oncorhynchus mykiss
1

Prat, Francisco; Coward, Kevin; Sumpter, John P.; Tyler, Charles R.

doi:10.1095/biolreprod58.5.1146

Cited by 77 publications

(49 citation statements)

References 38 publications

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“…The molecular masses of VtgAar and Lrp13 were estimated as described above. expressed in ovary, lr8 Ϫ and lr8 + gene expression has been detected by RT-PCR in almost all somatic tissues of several other egg-laying vertebrates ( 13,19,20,44,45 ). Less sensitive methods of detection (i.e., Northern blotting) in these same studies failed to detect lr8 Ϫ gene transcripts in somatic tissues, indicating that such ectopic expression is minor.…”

Section: Ligand and Western Blottingmentioning

confidence: 93%

“…We reported the molecular identity of a white perch Vtgr that is orthologous to mammalian VLDLR ( 18 ). This Vtgr is termed "Lr8 Ϫ ", because it is a spliced variant gene transcript of vldlr that does not encode the O -linked sugar domain, as is characteristic of this form of Vldlr in fi shes and chickens ( 19,20 ). Herein, we refer to these Vldlr forms as Lr8+ and Lr8 Ϫ , based on the presence or absence of the O -linked sugar domain, respectively.…”

Section: Protein-affi Nity Chromatographymentioning

confidence: 99%

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Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes

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Hiramatsu

Schilling

et al. 2014

Journal of Lipid Research

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Section: Ligand and Western Blottingmentioning

confidence: 93%

Section: Protein-affi Nity Chromatographymentioning

confidence: 99%

Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes

Reading

Hiramatsu

Schilling

et al. 2014

Journal of Lipid Research

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“…It has been established that in oviparous vertebrates, Vtgr mediates the entrance of Vtg into oocytes, although in birds and amphibians this receptor may also play a part in the incorporation of very low density lipoproteins (VLDL) [3], and this notion has recently been adopted for fish [4,5]. With the advent of cloning technology, identification and sequencing of the vgtr has been reported for a handful of fish species, which maintain highly conserved regions; however, two cDNA isoforms have been reported in rainbow trout [3,6], blue tilapia [7], and sole [8].…”

Section: Introductionmentioning

confidence: 99%

“…With the advent of cloning technology, identification and sequencing of the vgtr has been reported for a handful of fish species, which maintain highly conserved regions; however, two cDNA isoforms have been reported in rainbow trout [3,6], blue tilapia [7], and sole [8]. The isoforms consist of a larger cDNA sequence containing an O-glycan domain and a shorter one devoid of this region.…”

Section: Introductionmentioning

confidence: 99%

Identification and Transcriptional Modulation of the Largemouth Bass, Micropterus salmoides, Vitellogenin Receptor During Oocyte Development by Insulin and Sex Steroids1

Domínguez¹,

Quattro²,

Denslow³

et al. 2012

Biology of Reproduction

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Fish vitellogenin synthesized and released from the liver of oviparous animals is taken up into oocytes by the vitellogenin receptor. This is an essential process in providing nutrient yolk to developing embryos to ensure successful reproduction. Here we disclose the full length vtgr cDNA sequence for largemouth bass (LMB) that reveals greater than 90% sequence homology with other fish vtgr sequences. We classify LMB Vtgr as a member of the low density lipoprotein receptor superfamily based on conserved domains and categorize as the short variant that is devoid of the O-glycan segment. Phylogenetic analysis places LMB Vtgr sequence into a well-supported monophyletic group of fish Vtgr. Real-time PCR showed that the greatest levels of LMB vtgr mRNA expression occurred in previtellogenic ovarian tissues. In addition, we reveal the effects of insulin, 17beta-estradiol (E 2 ), and 11-ketotestosterone (11-KT) in modulation of vtgr, esr, and ar mRNAs in previtellogenic oocytes. Insulin increased vtgr expression levels in follicles ex vivo while exposure to E 2 or 11-KT did not result in modulation of expression. However, both steroids were able to repress insulin-induced vtgr transcript levels. Coexposure with insulin and E 2 or of insulin and 11-KT increased ovarian esr2b and ar mRNA levels, respectively, which suggest a role for these nuclear receptors in insulin-mediated signaling pathways. These data provide the first evidence for the ordered stage-specific expression of LMB vtgr during the normal reproductive process and the hormonal influence of insulin and sex steroids on controlling vtgr transcript levels in ovarian tissues.insulin, oocyte development, steroid hormones, vitellogenin, vitellogenin receptor

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“…In the fish ovary, lipoproteins (including Vtgs) are presumably sequestered via receptor-mediated uptake by the developing oocytes (17,45,65). Vtgs, the principal carriers of retinoids and carotenoids, are female-specific hepatically synthesized lipoproteins.…”

mentioning

confidence: 99%

Insight into molecular pathways of retinal metabolism, associated with vitellogenesis in zebrafish

Levi

Ziv

Admon

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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Levi L, Ziv T, Admon A, Levavi-Sivan B, Lubzens E. Insight into molecular pathways of retinal metabolism, associated with vitellogenesis in zebrafish. Am J Physiol Endocrinol Metab 302: E626 -E644, 2012. First published December 28, 2011; doi:10.1152/ajpendo.00310.2011Retinal is the main retinoid stored in oviparous eggs of fish, amphibians, and reptiles, reaching the oocytes in association with vitellogenins, the yolk precursor proteins. During early presegmentation stages of zebrafish embryos, retinal is metabolized to retinoic acid (RA), which regulates genes involved in cell proliferation, differentiation, and tissue function and is therefore essential for normal embryonic development. While synthesis of vitellogenin and its regulation by 17␤-estradiol (E2) were extensively investigated, pathways for retinal synthesis remain obscure. We determined the expression pattern of 46 candidate genes, aiming at identifying enzymes associated with retinal synthesis, ascertaining whether they were regulated by E2, and finding pathways that could fulfill the demand for retinoids during vitellogenesis. Genes associated with retinal synthesis were upregulated in liver (rdh10, rdh13, sdr) and surprisingly also in intestine (rdh13) and ovary (rdh1, sdr), concomitantly with higher gene expression and synthesis of vitellogenins in liver but also in extrahepatic tissues, shown here for the first time. Vitellogenin synthesis in the ovary was regulated by E 2. Gene expression studies suggest that elevated retinal synthesis in liver, intestine, and ovary also depends on cleavage of carotenoids (by Bcdo2 or Bmco1), but in the ovary it may also be contingent on higher uptake of retinol from the circulatory system (via Stra6) and retinol synthesis from retinyl esters (by Lpl). Decrease in oxidation (by Raldh2 or Raldh3) of retinal to RA and/or degradation of RA (by Cyp26a1) may also facilitate higher hepatic retinal levels. Together, these processes enable meeting the putative demands of retinal for binding to vitellogenins. Bioinformatic tools reveal multiple hormone response elements in the studied genes, suggesting complex and intricate regulation of these processes. extrahepatic vitellogenin synthesis; regulatory response elements; retinoids; carotenoids RETINAL IS THE PREDOMINANT RETINOID in eggs and oocytes of marine fish and some freshwater fish species and may constitute almost the entire pool of retinoids in these eggs. In oviparous eggs of nonmammalian vertebrates such as teleosts, amphibians, and birds, maternally derived retinal is bound via a Schiff-base linkage to vitellogenins (Vtgs) (15, 32, 62, 63, 64, 72 reviewed in 50). The retinal in fish oocytes and eggs consists of several isomers, whereas the all-trans form is the most abundant in zebrafish embryos (15,44). The use of the all-trans isomer of retinal for major retinoid storage is unique to reptiles, anamniotic vertebrates (teleosts and amphibians), and eggs of ascidian (33,34). This is in contrast to higher vertebrates (birds and mammals), where retinol is the...

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Molecular Characterization and Expression of two Ovarian Lipoprotein Receptors in the Rainbow Trout, Oncorhynchus mykiss 1

Cited by 77 publications

References 38 publications

Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes

Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes

Identification and Transcriptional Modulation of the Largemouth Bass, Micropterus salmoides, Vitellogenin Receptor During Oocyte Development by Insulin and Sex Steroids1

Insight into molecular pathways of retinal metabolism, associated with vitellogenesis in zebrafish

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