Molecular characterization and expression of sevenless, a gene involved in neuronal pattern formation in the Drosophila eye

Banerjee, Utpal; Renfranz, Patricia J.; Pollock, John A.; Benzer, Seymour

doi:10.1016/0092-8674(87)90569-1

Cited by 160 publications

(79 citation statements)

References 21 publications

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“…The sev protein product, a transmembrane receptor with a tyrosine kinase domain (Hafen et al 1987), is essential for the development of a single class of photoreceptor cells in the compound eye (Tomlinson and Ready 1986;Banerjee et al 1987). Furthermore, cell communication seems to be essential for developmental decisions within the "neurogenic region" of the Drosophila blastoderm stage embryo (for review, see Campos-Ortega and Hartenstein 1985).…”

Section: Discussionmentioning

confidence: 99%

Dorsal and neural expression of a tyrosine kinase-related Drosophila gene during embryonic development.

Haller

Côté²,

Brönner³

et al. 1987

Genes Dev.

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Section: Discussionmentioning

confidence: 99%

Dorsal and neural expression of a tyrosine kinase-related Drosophila gene during embryonic development.

Haller

Côté²,

Brönner³

et al. 1987

Genes Dev.

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“…For autosomal inserts, male transformants were assayed, whereas for X-linked inserts, female transformants were as-sayed to avoid dosage compensation effects (Spradling and Rubin 1983;Hazelrigg et al 1984). Standard genetic crosses were employed to produce transformant flies of the appropriate mutant genotypes using the following stocks: sevd2; ry s°6 (sevd~: Banerjee et al 1987), boss 3991 ry s°6 (boss3991: , gl 3 ry sa6 (glS: Moses et al 1989), so;TM3ryS°6/ + (so: Lindsley and Grell 1968), cm ct 6 oc ptg/CIB; ry s°6 (oc: Lindsley and Grell 1968) and In(2LR)noc 4 b cn sp/CyO; ry s°6 (noc4: Ashburner et al 1983).…”

Section: Ca T Enzyme Activity Assaysmentioning

confidence: 99%

Analysis of cis-acting requirements of the Rh3 and Rh4 genes reveals a bipartite organization to rhodopsin promoters in Drosophila melanogaster.

Fortini

Rubin²

1990

Genes Dev.

218

162

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The rhodopsin genes of Drosophila melanogaster are expressed in nonoverlapping subsets of photoreceptor cells within the insect visual system. Two of these genes, Rh3 and Rh4, are known to display complementary expression patterns in the UV-sensitive R7 photoreceptor cell population of the compound eye. In addition, we find that Rh3 is expressed in a small group of paired R7 and R8 photoreceptor cells at the dorsal eye margin that are apparently specialized for the detection of polarized light. In this paper we present a detailed characterization of the cis-acting requirements of both Rh3 and Rh4. Promoter deletion series demonstrate that small regulatory regions (<300 bp) of both R7 opsin genes contain DNA sequences sufficient to generate their respective expression patterns. Individual cis-acting elements were further identified by oligonucleotidedirected mutagenesis guided by interspecific sequence comparisons. Our results suggest that the Drosophila rhodopsin genes share a simple bipartite promoter structure, whereby the proximal region constitutes a functionally equivalent promoter "core" and the distal region determines cell-type specificity. The expression patterns of several hybrid rhodopsin promoters, in which all or part of the putative core regions have been replaced with the analogous regions of different rhodopsin promoters, provide additional evidence in support of this model.

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“…Two methods were used to screen for eye mutations. To search for abnormalities in the in ternal arrangement of R cells, the optical method of deep pseudopupil (Franceschini and Kirschfeld 1971) was adapted for rapid screening of large numbers of flies (Banerjee et al 1987a). In addition, a visual screen under a dissecting microscope was used to detect changes in the external eye morphology.…”

Section: Isolation and Mapping Of Rapmentioning

confidence: 99%

Neuronal development in the Drosophila compound eye: rap gene function is required in photoreceptor cell R8 for ommatidial pattern formation.

Karpilow¹,

Kolodkin²,

Bork³

et al. 1989

Genes & Development

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In the compound eye of Drosopbila, cell-cell interactions are thought to play an important role in the determination of neuronal cell fate and pattern morphogenesis. Recent work on the bride of sevenless {boss) gene has demonstrated an inductive role for photoreceptor R8 in the differentiation of photoreceptor R7. These studies have shown that while R8 differentiates early in the scheme of ommatidial assembly, it continues to play an active role in subsequent patterning events. We describe studies on a new genetic locus rap {retina aberrant in pattern), whose functions are critical for normal pattern formation in the developing eye. Mutations in the rap gene perturb the early stages of pattern formation and lead to a variable number of photoreceptor cells (R cells) in each ommatidium. Experiments with a temperature-sensitive allele have shown that rap gene function is required during the period of development when pattern formation occurs. In addition, a somatic mosaic analysis of rap has shown that its function is required only in photoreceptor cell R8 for normal ommatidial patterning. These studies suggest an important role for rap in the initial events leading to pattern formation and are consistent with R8 playing a central role in directing ommatidial pattern formation.

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Molecular characterization and expression of sevenless, a gene involved in neuronal pattern formation in the Drosophila eye

Cited by 160 publications

References 21 publications

Dorsal and neural expression of a tyrosine kinase-related Drosophila gene during embryonic development.

Dorsal and neural expression of a tyrosine kinase-related Drosophila gene during embryonic development.

Analysis of cis-acting requirements of the Rh3 and Rh4 genes reveals a bipartite organization to rhodopsin promoters in Drosophila melanogaster.

Neuronal development in the Drosophila compound eye: rap gene function is required in photoreceptor cell R8 for ommatidial pattern formation.

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