Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)

Feng, Xiu; Yu, Xin; Pang, Maolin; Liu, Haiyang; Tong, Jingou

doi:10.1016/j.cbpb.2014.12.001

Cited by 14 publications

(5 citation statements)

References 71 publications

(97 reference statements)

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“…The candidate gene approach provides a powerful tool to study the genetic architecture of complex quantitative traits in aquatic animals [ 38 , 39 ]. The association study between specific alleles of single-nucleotide polymorphisms (SNPs) detected within a candidate gene and the trait of interest is a common strategy to elucidate quantitative trait nucleotides (QTN) and major genes that may affect quantitative polygenic traits, and the method has been successfully applied to several growth candidate genes in common carp [ 40 , 41 , 42 ]. Compared with grass carp [ 20 ], large numbers of SNPs ( Table 1 ) in the present study may indicate that the polymorphic levels of C. carpio a poA-Ibs are high, which may be due to the tetraploidization of the common carp genome in evolution [ 22 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Growth Association of Two Apolipoprotein A-Ib Genes in Common Carp (Cyprinus carpio)

Wang

Tong

2016

IJMS

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Apolipoprotein A-I (ApoA-I) is functionally involved in the transportation and metabolism of lipids in vertebrates. In this study, two isoforms of apoA-Ib in common carp (Cyprinus carpio L.) were characterized. Sequence comparison and phylogenetic analysis showed that C. carpio ApoA-Ib is relatively conserved within cyprinid fishes. During embryonic development, C. carpio apoA-Ib was first expressed at the stage of multi-cells, and the highest mRNA level was observed at the stage of optic vesicle. A ubiquitous expression pattern was detected in various tissues with extreme predominance in the liver. Significantly different expression levels were observed between light and heavy body weight groups and also in the compensatory growth test. Seventeen and eight single-nucleotide polymorphisms (SNPs) were identified in matured mRNA of the C. carpio apoA-Ib.1 and apoA-Ib.2, respectively. Two of these SNPs (apoA-Ib.2-g.183A>T and apoA-Ib.2-g.1753C>T) were significantly associated with body weight and body length in two populations of common carp. These results indicate that apoA-Ib may play an important role in the modulation of growth and development in common carp.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Growth Association of Two Apolipoprotein A-Ib Genes in Common Carp (Cyprinus carpio)

Wang

Tong

2016

IJMS

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“…qPCR was performed on Bio-Rad iQ5 System with SYBR Premix Ex Taq™ kit according to the manual (Takara). The relative expression level of chGMII in different tissues were calculated using the 2 −△△Ct method and subject to a one-way analysis of variance (one-way ANOVA) using SPSS 13.0 software [21]. The results were presented as mean ± standard error, and differences were considered significant at P < 0.05.…”

Section: Real-time Qpcrmentioning

confidence: 99%

Cloning, Expression, and Characterization of Capra hircus Golgi α-Mannosidase II

Zhang

Lai

et al. 2015

Appl Biochem Biotechnol

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Golgi α-mannosidase II (GMII), a key glycosyl hydrolase in the N-linked glycosylation pathway, has been demonstrated to be closely associated with the genesis and development of cancer. In this study, we cloned cDNA-encoding Capra hircus GMII (chGMII) and expressed it in Pichia pastoris expression system. The chGMII cDNA contains an open reading frame of 3432 bp encoding a polypeptide of 1144 amino acids. The deduced molecular mass and pI of chGMII was 130.5 kDa and 8.04, respectively. The gene expression profile analysis showed GMII was the highest expressed gene in the spleen. The recombinant chGMII showed maximum activity at pH 5.4 and 42 °C and was activated by Fe(2+), Zn(2+), Ca(2+), and Mn(2+) and strongly inhibited by Co(2+), Cu(2+), and EDTA. By homology modeling and molecular docking, we obtained the predicted 3D structure of chGMII and the probable binding modes of chGMII-GnMan5Gn, chGMII-SW. A small cavity containing Tyr355 and zinc ion fixed by residues Asp290, His176, Asp178, and His570 was identified as the active center of chGMII. These results not only provide a clue for clarifying the catalytic mechanism of chGMII but also lay a theoretical foundation for subsequent investigations in the field of anticancer therapy for mammals.

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“…The SST2 gene, also known as cortistatin (CORT) in mammals, is expressed in many vertebrates such as human (Homo sapiens) [9,10], chicken (Gallus gallus) [11], and African lungfish (Protopterus annectens) [12]. In grouper (Epinephelus coioides) [13] and common carp (Cyprinus carpio) [14], three SST genes (SST1, SST3, and SST6) have been observed. In dogfish (Scylorhinus canicula) [15] and lamprey (Lampetra japonicum) [16], five SST genes (SST1, SST2, SST3, SST5, and SST6) and three SST genes (SSTa, SSTb, and SSTc) have been observed, respectively, although the phylogenetic relationship of the three lamprey SST genes remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Identification, Expression, and Functions of the Somatostatin Gene Family in Spotted Scat (Scatophagus argus)

Feng

Tian

Lin

et al. 2020

Genes

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Somatostatins (SSTs) are a family of proteins consisting of structurally diverse polypeptides that play important roles in the growth regulation in vertebrates. In the present study, four somatostatin genes (SST1, SST3, SST5, and SST6) were identified and characterized in the spotted scat (Scatophagus argus). The open reading frames (ORFs) of SST1, SST3, SST5, and SST6 cDNA consist of 372, 384, 321, and 333 bp, respectively, and encode proteins of 123, 127, 106, and 110 amino acids, respectively. Amino acid sequence alignments indicated that all SST genes contained conserved somatostatin signature motifs. Real-time PCR analysis showed that the SST genes were expressed in a tissue specific manner. When liver fragments were cultured in vitro with synthetic peptides (SST1, SST2, or SST6 at 1 μM or 10 μM) for 3 h or 6 h, the expression of insulin-like growth factor 1 and 2 (Igf-1 and Igf-2) in the liver decreased significantly. Treatment with SST5 had no significant effect on Igf-1 and Igf-2 gene expression. This study provides an enhanced understanding of the gene structure and expression patterns of the SST gene family in S. argus. Furthermore, this study provides a foundation for future exploration into the role of SST genes in growth and development.

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Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)

Cited by 14 publications

References 71 publications

Molecular Characterization and Growth Association of Two Apolipoprotein A-Ib Genes in Common Carp (Cyprinus carpio)

Molecular Characterization and Growth Association of Two Apolipoprotein A-Ib Genes in Common Carp (Cyprinus carpio)

Cloning, Expression, and Characterization of Capra hircus Golgi α-Mannosidase II

Identification, Expression, and Functions of the Somatostatin Gene Family in Spotted Scat (Scatophagus argus)

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