Molecular characterization and expression dynamics of MTP genes under various spatio-temporal stages and metal stress conditions in rice

Ram, Hasthi; Kaur, Amandeep; Gandass, Nishu; Singh, Shweta; Deshmukh, Rupesh; Sonah, Humira; Sharma, T. R.

doi:10.1371/journal.pone.0217360

Cited by 37 publications

(25 citation statements)

References 31 publications

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“…Under different heavy metal (Co, Cd, Ni) stress conditions, downregulation of most TaVTL genes was observed in roots and up‐regulation of most of the TaVTL genes was observed under Co stress. A similar trend in expression (downregulation in roots and upregulation in shoots) has been observed for many Metal Tolerance Protein ( MTP ) genes under different heavy metal stress conditions (Ram et al, 2019). These results highlight important role of TaVTL genes in heavy metal stress tolerance in wheat.…”

Section: Vit and Vit‐like (Vtl) Transporters In Different Plant Speciessupporting

confidence: 65%

Vacuolar Iron Transporter (Like) proteins: Regulators of cellular iron accumulation in plants

Ram

Sardar

Gandass

2021

Physiologia Plantarum

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Iron is not only important for plant physiology, but also a very important micronutrient in human diets. The vacuole is the main site for accumulation of excess amounts of various nutrients and toxic substances in plant cells. During the past decade, many Vacuolar Iron Transporter (VIT) and VIT‐Like (VTL) genes have been identified and shown to play important roles in iron homeostasis in different plants. Furthermore, recent reports identified novel roles of these transporter genes in symbiotic nitrogen fixation (SNF) in legume crops as well as in the blue coloration of petals in flowers. The literature indicates their universal role in Fe transport across different tissues (grains, nodules, flowers) to different biological processes (cellular iron homeostasis, SNF, petal coloration) in different plants. Here, we have systematically reviewed different aspects, such as structure, molecular evolution, expression, and function of VIT/VTL proteins. This will help future studies aimed at functional analysis of VIT/VTL genes in other plant species, vacuolar transportation mechanisms, and iron biofortification at large.

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Section: Vit and Vit‐like (Vtl) Transporters In Different Plant Speciessupporting

confidence: 65%

Vacuolar Iron Transporter (Like) proteins: Regulators of cellular iron accumulation in plants

Ram

Sardar

Gandass

2021

Physiologia Plantarum

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“…Compared with that of previous report, three new MTP genesn were found in P. trichocarpa [20]. The number of MTPs in P. trichocarpa was only second to N. tabacum among the plant species in which the MTPs have been identified [5,8,[22][23][24][25][26][27][28]. However, despite the large number of PtrMTPs, the homolog of AtMTP2 in P. trichocarpa was not detected.…”

Section: Evolution and Differentiation Of Ptrmtp Genes As Well As Thecontrasting

confidence: 62%

Genome-Wide Identification of Metal Tolerance Protein Genes in Populus trichocarpa and Their Roles in Response to Various Heavy Metal Stresses

Yang

Liu

et al. 2020

IJMS

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Metal tolerance proteins (MTPs) are plant divalent cation transporters that play important roles in plant metal tolerance and homeostasis. Poplar is an ideal candidate for the phytoremediation of heavy metals because of its numerous beneficial attributes. However, the definitive phylogeny and heavy metal transport mechanisms of the MTP family in poplar remain unknown. Here, 22 MTP genes in P. trichocarpa were identified and classified into three major clusters and seven groups according to phylogenetic relationships. An evolutionary analysis suggested that PtrMTP genes had undergone gene expansion through tandem or segmental duplication events. Moreover, all PtrMTPs were predicted to localize in the vacuole and/or cell membrane, and contained typical structural features of the MTP family, cation efflux domain. The temporal and spatial expression pattern analysis results indicated the involvement of PtrMTP genes in poplar developmental control. Under heavy metal stress, most of PtrMTP genes were induced by at least two metal ions in roots, stems or leaves. In addition, PtrMTP8.1, PtrMTP9 and PtrMTP10.4 displayed the ability of Mn transport in yeast cells, and PtrMTP6 could transport Co, Fe and Mn. These findings will provide an important foundation to elucidate the biological functions of PtrMTP genes, and especially their role in regulating heavy metal tolerance in poplar.

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“…The predicted results of the subcellular localization indicated that all StMTPs are located in the vacuole which agreed with the results of Gao (2020). The predicted results of the TMHMM Server v. 2.0 analysis showed that StMTP4 has a transmembrane domain that is similar to OsMTP6 (without the domain) (Ram et al 2019). StMTP2 has 12 transmembrane domains similar to 14 in Brassica rapa var.…”

Section: The Expression Patterns Of Stmtp In Different Tissues Under supporting

confidence: 82%

Genome-Wide Identification of Metal Tolerance Genes in Potato (Solanum Tuberosum): Response to Two Heavy Metal Stress

Tian

et al. 2021

Preprint

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Metal tolerance proteins play an important role in the transport and tolerance of divalent heavy metals in plant species. Potatoes are an important food crop whose yields can be deeply affected by heavy metals. However, there is a lack of information concerning the members and function of the MTP gene family in Solanum tuberosum. In this study, we identified and screened 11 MTP genes in potatoes which we named as StMTP1 to StMTP11 based on their positions on the chromosomes. Phylogenetic analysis divided these 11 MTP genes into three subfamilies; Mn-MTP, Zn-MTP and Zn/Fe-MTP. HXXXD and DXXXD conserved motifs were found on or around the transmembrane domain II and transmembrane domain V of these proteins. The highly conserved histidine and aspartic acid residues may be related to the transport of metal ions. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression levels of StMTP9 and StMTP10 in leaf tissues increased by around 24-fold following cadmium stress for 24 hours. We hypothesize that StMTP9 and StMTP10 respond to cadmium stress. StMTP11 showed the highest level of expression in stem tissues after 6 hours of zinc stress at more than 13 times the level of expression in controls indicating that StMTP11 is more sensitive to zinc stress. In summary, our results further the current understanding of the molecular mechanisms regulated by members of the MTP gene family in plant responses to heavy metal stress.

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Molecular characterization and expression dynamics of MTP genes under various spatio-temporal stages and metal stress conditions in rice

Cited by 37 publications

References 31 publications

Vacuolar Iron Transporter (Like) proteins: Regulators of cellular iron accumulation in plants

Vacuolar Iron Transporter (Like) proteins: Regulators of cellular iron accumulation in plants

Genome-Wide Identification of Metal Tolerance Protein Genes in Populus trichocarpa and Their Roles in Response to Various Heavy Metal Stresses

Genome-Wide Identification of Metal Tolerance Genes in Potato (Solanum Tuberosum): Response to Two Heavy Metal Stress

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