Molecular characterization and expression analysis of three hypoxia-inducible factor alpha subunits, HIF-1α/2α/3α of the hypoxia-sensitive freshwater species, Chinese sucker

Chen, Nan; Chen, Li Ping; Zhang, Jie; Chen, Cheng; Wei, Xin; Gul, Yasmeen; Wang, Weimin; Wang, Huan Ling

doi:10.1016/j.gene.2011.12.058

Cited by 60 publications

(48 citation statements)

References 35 publications

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“…The hypoxia experiments revealed a clear pattern of increased hepatic hif-1α expression under oxygen conditions around 2 mg L − 1 , similar to studies on Eurasian perch (Perca fluviatilis), sea bass (Dicentrarchus labrax), and Chinese sucker (Terova et al, 2008;Chen et al, 2012;Rimoldi et al, 2012). In addition, mRNA expression returning to baseline levels within 24 hours is also consistent across multiple studies and species, tolerant and sensitive alike Terova et al, 2008;Kodama et al, 2012b;Rimoldi et al, 2012).…”

Section: Discussionsupporting

confidence: 81%

“…HIF-1α stabilizes when oxygen is limited, resulting in a cascade of gene regulation (200+ genes) and associated biological effects (Bruick, 2003;Wenger, 2006). While regulation of the post-translational form of HIF-1α (i.e., protein) is well understood, the mechanism governing hif-1α gene expression (i.e., mRNA) is less clear, particularly for fish (Chen et al, 2012). However, laboratory studies of aquatic organisms have shown evidence of increased hif-1α gene expression in response to low oxygen levels (Bracken et al, 2003;Thomas et al, 2007;Thomas and Rahman, 2009;Shen et al, 2010;Kodama et al, 2012aKodama et al, , 2012b.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, we used a hepatic transcriptome of Pacific herring to develop a qPCR assay for hif-1α. Liver tissue was selected because of the rapid cellular turnover (potentially most responsive to acute exposure), elevated hif-1α detection in other species (Terova et al, 2008;Chen et al, 2012;Rimoldi et al, 2012), and the sequenced transcriptome (S. B. Roberts et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Evaluating hypoxia-inducible factor-1α mRNA expression in a pelagic fish, Pacific herring Clupea pallasii, as a biomarker for hypoxia exposure

Froehlich¹,

Roberts²,

Essington³

2015

Comparative Biochemistry and Physiology Part A: Molecular &

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Hypoxia [dissolved oxygen (DO)<2 mg L(-1)] is a major environmental perturbation for many aquatic ecosystems, particularly highly productive estuaries. Most research attention and understanding about the impacts of hypoxia on estuarine species has focused on the benthos, where hypoxia is most common. Although the pelagic zone is also susceptible to the effects of hypoxia, the biological interactions and consequences are not as well understood in marine environments because documenting exposure or avoidance of hypoxia is often difficult. Physiological biomarkers may provide a way to gain more detailed spatiotemporal information regarding species' exposure to hypoxia. Here, we identified and tested a hypoxia-specific responsive gene, hypoxia-inducible factor-1α (hif-1α), to evaluate its potential as a biomarker for hypoxia exposure in Pacific herring (Clupea pallasii). We conducted controlled laboratory experiments to establish the level of hepatic hif-1α elevated gene expression (>1 sd normoxic mean), exposure amplification (≥2 hours), reduction rate (ca. 24 hours), and some evidence of a lethal hypoxic limit (ca. 2 mg L(-1), ≥4 hours). We then used these findings to evaluate the spatiotemporal patterns of hif-1α for Pacific herring in a seasonally hypoxia estuary, Hood Canal, Washington, USA. Although expression did not parallel the local hypoxic conditions in the estuary, herring from the more severe hypoxic year (2013) had a higher probability of having elevated mRNA levels. These patterns indicate that hepatic hif-1α levels may not be directly indicative of local DO levels for pelagic marine fish, but rather provide insight into hypoxia exposure over broader scales.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluating hypoxia-inducible factor-1α mRNA expression in a pelagic fish, Pacific herring Clupea pallasii, as a biomarker for hypoxia exposure

Froehlich¹,

Roberts²,

Essington³

2015

Comparative Biochemistry and Physiology Part A: Molecular &

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“…The spatiotemporal expression pattern of Hif-3␣1 was also studied in zebrafish and other fishes (55,40,41,25,2). During embryogenesis, Hif-3␣1 mRNA became detectable by RT-PCR as early as 4 h post fertilization (hpf).…”

Section: Spatial and Temporal Expression Of Hif-3a Variantsmentioning

confidence: 99%

Hypoxia-inducible factor 3 biology: complexities and emerging themes

Duan

2016

American Journal of Physiology-Cell Physiology

194

156

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Duan C. Hypoxia-inducible factor 3 biology: complexities and emerging themes. Am J Physiol Cell Physiol 310: C260 -C269, 2016. doi:10.1152/ajpcell.00315.2015.-The hypoxia-inducible factor (HIF) family has three distinct members in most vertebrates. All three HIFs consist of a unique and oxygen-labile ␣-subunit and a common and stable ␤-subunit. While HIF-1 and HIF-2 function as master regulators of the transcriptional response to hypoxia, much less is known about HIF-3. The HIF-3␣ gene gives rise to multiple HIF-3␣ variants due to the utilization of different promoters, different transcription initiation sites, and alternative splicing. These HIF-3␣ variants are expressed in different tissues, at different developmental stages, and are differentially regulated by hypoxia and other factors. Recent studies suggest that different HIF-3␣ variants have different and even opposite functions. There is strong evidence that full-length HIF-3␣ protein functions as an oxygen-regulated transcription activator and that it activates a unique transcriptional program in response to hypoxia. Many HIF-3␣ target genes have been identified. While some short HIF-3␣ variants act as dominant-negative regulators of HIF-1/2␣ actions, other HIF-3␣ variants can inhibit HIF-1/2␣ actions by competing for the common HIF-␤. There are also a number of HIF-3␣ variants yet to be explored. Future studies of these naturally occurring HIF-3␣ variants will provide new and important insights into HIF biology and may lead to the development of new therapeutic strategies.hypoxia-inducible factor; alternative splicing; oxygen-dependent degradation; gene expression OXYGEN IS AN ESSENTIAL MOLECULE required for cellular respiration and efficient energy production. Hypoxia, a condition in which an organism or a cell is deprived of adequate oxygen (e.g., when oxygen demand exceeds oxygen supply), occurs in developing embryos and adult tissues and plays key roles in stem cell biology, development, and adult physiology. Hypoxia is also frequently associated with pathological states such as ischemia, arthritis, inflammation, and tumorigenesis. Hypoxia triggers complex adaptive responses and activates the expression of numerous genes. These hypoxia-responsive genes are involved in changes in erythropoiesis, angiogenesis, metabolic reprograming, cell-cycle regulation, and tumorigenesis (9,39,(43)(44)(45). The transcriptional response to hypoxia is coordinated by hypoxiainducible factors (HIFs). HIFs are heterodimers, consisting of an oxygen-labile HIF␣ subunit and a stable HIF-␤ subunit [also known as aryl hydrocarbon receptor nuclear translocator (ARNT)]. In human and other vertebrate species, there are three distinct HIF␣ genes. Much of our knowledge about HIF signaling is derived from studies on HIF-1␣ and HIF-2␣ (9, 39, 43-45). Both HIF-1␣ and HIF-2␣ contain a basic helix-loophelix (bHLH) domain, two Per-Arnt-Sim (PAS) domains, a PAS-associated COOH-terminal (PAC) domain; an oxygendependent degradation (ODD) domain; an NH 2 -terminal transactivation domain (N...

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“…For example, in grass carp (Ctenopharyngodon idella), two distinct HIF-α isoforms (gcHIF-1alpha and gcHIF-4alpha) were differentially regulated at the transcriptional level in response to hypoxic stress in different organs (Law et al, 2006). Similar studies were performed in Atlantic croaker (Micropogonias undulatus), Chinese sucker (Myxocyprinus asiaticus) and Wuchang bream (Megalobrama amblycephala) and also showed that HIF-α isoforms were regulated in hypoxic conditions at the transcriptional level and potentially useful molecular indicators of environmental hypoxia exposure (Chan et al, 2005;Rahman and Thomas, 2007;Chen et al, 2012). Nevertheless, the contribution of HIF and the genetics basis to high-altitude adaptation in Triplophysa fishes were yet to be investigated.…”

Section: Introductionmentioning

confidence: 61%

Transcriptome analysis of the plateau fish (Triplophysa dalaica): Implications for adaptation to hypoxia in fishes

Wang

Yang

et al. 2015

Gene

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Molecular characterization and expression analysis of three hypoxia-inducible factor alpha subunits, HIF-1α/2α/3α of the hypoxia-sensitive freshwater species, Chinese sucker

Cited by 60 publications

References 35 publications

Evaluating hypoxia-inducible factor-1α mRNA expression in a pelagic fish, Pacific herring Clupea pallasii, as a biomarker for hypoxia exposure

Evaluating hypoxia-inducible factor-1α mRNA expression in a pelagic fish, Pacific herring Clupea pallasii, as a biomarker for hypoxia exposure

Hypoxia-inducible factor 3 biology: complexities and emerging themes

Transcriptome analysis of the plateau fish (Triplophysa dalaica): Implications for adaptation to hypoxia in fishes

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