Molecular characterization and expression analysis of five chitinases associated with molting in the Chinese mitten crab, Eriocheir sinensis

Li, Xuguang; Xu, Zhiqiang; Zhou, Gang; Lin, Hai; Zhou, Jun; Zeng, Qiandong; Mao, Zhigang; Gu, Xiaohong

doi:10.1016/j.cbpb.2015.05.007

Cited by 56 publications

(43 citation statements)

References 35 publications

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“…The selected genes included chitinase, alkaline phosphatase, insulin receptor, juvenile hormone esterase and signal transducer and activator of transcription, and these genes have been demonstrated to be related to the functions of growth and development in many crustaceans (Chen et al . ; Boucher, Ditlecadet & Dubé ; Lee, Jeon, Ohc, Kim, Kang, Lee, Mykles & Kim ; Okugawa, Mekata & Inada ; Li, Xu, Zhou, Lin, Zhou, Zeng, Mao & Gu ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…These results suggest important roles of these chitinase genes for the metamorphosis, growth and reproduction of the Chinese mitten crabs (Li et al . ). Therefore, it can be speculated that the upregulated expression of chitinases in hepatopancreas of the precocious juvenile crabs might be of special physiological significance for the gonad development, and which might be associated with the precocity of the Chinese mitten crab.…”

Section: Discussionmentioning

confidence: 97%

“…As expected, we identified a plenty of differentially expressed genes from the RNA-Seq (Quantification) analysis. These differentially expressed genes, including chitinase, alkaline phosphatase, insulin receptor, juvenile hormone esterase (JHE) and signal transducer and activator of transcription (STAT), have long been reported to be related to the growth or development in many other crustaceans (Chen et al 2005;Boucher et al 2010;Lee et al 2011;Okugawa et al 2013;Li et al 2015).…”

Section: Discussionmentioning

confidence: 99%

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Comparative transcriptome sequencing of the hepatopancreas reveals differentially expressed genes in the precocious juvenile Chinese mitten crab,Eriocheir sinensis(Crustacea: Decapoda)

Deng

et al. 2016

Aquac Res

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Overnutrition was considered to be the main cause of the precocity in the Chinese mitten crab. Hepatopancreas plays a key role in nutrition metabolism of the crustaceans. However, gene regulation and expression profiles of the hepatopancreas for the precocious crabs still remain ill‐defined. Here through de novo sequencing coupled with RNA‐Seq (Quantification) analysis, a number of differentially expressed genes (DEGs) in the precocious juvenile crabs were identified. The pooled total RNA from hepatopancreas of the precocious juvenile crabs, normal juvenile crabs and normal matured crabs was used to obtain a reference transcriptome. De novo assembly of the transcriptome revealed 55 561 unigenes with an average length of 1153 bp. Another three cDNA libraries were constructed with the same samples and sequenced separately with the RNA‐Seq analysis. A total of 996 DEGs between the precocious and normal juvenile crabs were identified, of which 508 DEGs were upregulated in the precocious juvenile crabs. KEGG enrichment analysis revealed that most enriched DEGs (162, 23.28%) were involved in the metabolic pathways. The expression profiles of 15 DEGs, which includes chitinase, alkaline phosphatase, insulin receptor, juvenile hormone esterase and signal transducer and activator of transcription, were determined by qRT‐PCR to confirm the accuracy of the RNA‐Seq. These DEGs had already been suggested to be potentially related to the growth and development in crustaceans. Our results, including the assembled transcriptome and the identified DEGs in the precocious juvenile crabs, provide us new resources in the understanding of the functions of the hepatopancreas in the precocity of the Chinese mitten crab.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Comparative transcriptome sequencing of the hepatopancreas reveals differentially expressed genes in the precocious juvenile Chinese mitten crab,Eriocheir sinensis(Crustacea: Decapoda)

Deng

et al. 2016

Aquac Res

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“…Furthermore, some chitinases have been found to play important roles in defences against chitin‐bearing pathogens, such as white spot syndrome virus (WSSV) in Fenneropenaeus chinensis (Zhang, Sun, Li, Huang & Xiang, ), Vibrio anguillarum and WSSV in Exopalaemon carinicauda (Duan et al., ), and Streptococcus agalactiae and Vibrio harveyi in Penaeus monodon (Zhou et al., ). Moreover, crustacean chitinases are involved in the digestion of chitinous food (Tan, Degnan & Lehnert, ), larval development (Li et al., ), modification of the peritrophic membrane (Salma et al., ), resistance to environmental stresses (Nikapitiya et al., ) and other processes.…”

Section: Introductionmentioning

confidence: 99%

“…The crustacean chitinase genes were first isolated and characterized in Marsupenaeus japonicus , and their roles during the moulting cycle were investigated (Watanabe & Kono, ; Watanabe, Kono, Aida & Nagasawa, , ). The chitinase genes were subsequently cloned in various crustacean species, including P. monodon (Proespraiwong et al., ; Tan et al., ; Zhou et al., ), F. chinensis (Priya et al., ; Zhang et al., ), Litopenaeus vannamei (Huang et al., ), Pandalopsis japonica (Salma et al., ), E. carinicauda (Duan et al., ), Macrobrachium nipponense (Zhang et al., ), M. japonicus (Nikapitiya et al., ; Park, Nikapitiya, Kim, Kwak & Kwak, ) and Eriocheir sinensis (Li et al., ). However, to date, no more than six enzyme activity motif‐containing chitinase transcripts have been identified in crustaceans (Huang et al., ; Li et al., ).…”

Section: Introductionmentioning

confidence: 99%

Seven transcripts from the chitinase gene family of the mud crabScylla paramamosain: Their expression profiles during development and moulting and under environmental stresses

Zhou

et al. 2018

Aquac Res

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In crustaceans, chitinases (Chts) play crucial roles in physiological functions such as chitinous food digestion, moulting and stress responses. Chitinases have been identified in both prokaryotes and eukaryotes, but the composition and number of Cht family members vary across species, especially in crustaceans. Seven Cht transcripts, all of which contained the conserved enzyme activity motif, were cloned from Scylla paramamosain for the first time, and SpCht1-SpCht6 were clearly classified into groups 1-6, respectively, through phylogenetic analysis. Notably, SpCht7, which had two catalytic domains, was identified for the first time in crabs and crustaceans.Although SpCht7 could not be grouped into any known Cht subclasses, it clustered together with insect Cht7 and could be classified into a novel subclass that we named group 7. The tissue distributions of SpChts, as well as their expression profiles during embryonic and larval development, growth-related moulting, salinity and cadmium stresses, were also investigated. The results suggested that SpChts might have different and varied biological functions. These results would be helpful for understanding and clarifying the roles of chitinases in crab development, moulting and responses to environmental stress. These results also provide new insight into the origin and evolution of the chitinase gene family in crabs and other crustaceans. K E Y W O R D Schitinase, development, moulting, Scylla paramamosain, stress

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Geometric Morphometric Analysis of the Chinese Mitten Crab Eriocheir sinensis: A Potential Approach for Geographical Origin Authentication

Zheng

Jiang

Luo

et al. 2021

N American J Fish Manag

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The illegal counterfeiting of the geographic origin label for Chinese mitten crabs Eriocheir sinensis is a major problem in China. More effective methods are needed for authenticating their geographic origin and increasing their commercial value. Therefore, a geometric morphometric analysis was conducted for the present pilot study to identify variation in Chinese mitten crabs from eight well‐known geographic areas in China: Gehu, Luoma, Gucheng, Liangzi, and Junshan lakes in the Yangtze River system; the Dongying area in the Yellow River system; and the Yingkou and Panjin areas in the Liaohe River system. Landmark coordinates were extracted by image processing after digitization of the carapace and the ventral region of the cephalothorax and were then analyzed using relative warp principal components methods, discriminant analysis, thin‐plate spline analysis, and grid distortion. Many of the type II landmarks used were extracted via relative warp principal components analysis, and they had the most important contributions to explaining the variation. The discriminant analysis indicated that the carapace of male and female crabs and the ventral region of the cephalothorax in males had geographical origin distinguishing rates of 100%. These results suggest that plasticity in shape exists for local adaptation of the ventral region of the cephalothorax and especially the carapace among Chinese mitten crabs from the different locations examined. Geometric morphometric analysis has potential for authenticating the important commercial crab species by authorized inspectors or scientists in markets or laboratories after further development of image processing, landmark extraction, shape morphometric databases, detection models, and geographical origin recognition.

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Molecular characterization and expression analysis of five chitinases associated with molting in the Chinese mitten crab, Eriocheir sinensis

Cited by 56 publications

References 35 publications

Comparative transcriptome sequencing of the hepatopancreas reveals differentially expressed genes in the precocious juvenile Chinese mitten crab,Eriocheir sinensis(Crustacea: Decapoda)

Comparative transcriptome sequencing of the hepatopancreas reveals differentially expressed genes in the precocious juvenile Chinese mitten crab,Eriocheir sinensis(Crustacea: Decapoda)

Seven transcripts from the chitinase gene family of the mud crabScylla paramamosain: Their expression profiles during development and moulting and under environmental stresses

Geometric Morphometric Analysis of the Chinese Mitten Crab Eriocheir sinensis: A Potential Approach for Geographical Origin Authentication

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