Molecular characterization and expression analysis of the gene coding for the porcine β3 integrin subunit (CD61)

Two cDNAs coding for the porcine CD51 (αv) integrin subunit: Cloning, expression analysis, adhesion assays and chromosomal localization

“…α v , in association with different β chains, has been involved in many different functions as osteoclastogenesis, bone resorption, migration and activation of macrophages, angiogenesis or apoptosis (Lessey et al, 1992;McHugh et al, 2000;Stupack et al, 2001;Staquet et al, 2006). In platelets, α v integrin must be expressed in association with β 3 , detected by the anti-β 3 mAb JM2E5 in this study, and previously detected by us with an anti-recombinant porcine β 3 antibody (Jiménez-Marín et al, 2008). Although the function of the α v β 3 complex in platelets is not known, some authors have reported that the complex is involved in the coagulation process (Katagiri et al, 1995).…”

Pulmonary Macrophages

“…Signal regulatory protein a (SIRPa) þþþ þ Gjomarkaj et al, 1999;Garn et al, 2006; Rouzer et al, 1982;Gant and Hamblin, 1985;Lehnert et al, 1990;Lehnert, 1992 Continued Prieto et al, 1994;Johansson et al, 1997;Frankenberger et al, 2000;Taylor et al, 2000;Garn et al, 2006;Johnston et al, 2012 CD61 b3 integrin subunit þ/À þ þ Pons et al, 2005;Jimenez-Marin et al, 2008;Schneberger et al, 2012 CD11b/CD18 CR3 (Mac-1) þþ þ þþþ þ Miller et al, 1992;Lohmann-Matthes et al, 1994;Prieto et al, 1994;Franke-Ullmann et al, 1996;Johansson et al, 1997;Gjomarkaj et al, 1999;Frankenberger et al, 2000;Zetterberg et al, 2000;Lagranderie et al, 2003;Pons et al, 2005;Lay et al, 2009;Misharin et al, 2013;Zaynagetdinov et al, 2013 CD11c CR4 a chain þþþ þ Taylor et al, 2000;Lagranderie et al, 2003;Grundy and Sentman, 2005;Bedoret et al, 2009;Misharin et al, 2013;Zaynagetdinov et al, 2013 Receptors Lehnert et al, 1990;Lehnert, 1992;Miller et al, 1992;Gjomarkaj et al, 1999;…”

“…In contrast, they exhibit similar phagocytic, bactericidal, tumoricidal, and antibody-dependent cytotoxic activity, and release comparable levels of IL-1 and TNFa (Chitko-McKown et al, 1991;Staub, 1994). Studies using gadolinium chloride and clodronate liposomes to deplete pulmonary intravascular macrophages have demonstrated a proinflammatory role of these cells in lung inflammation induced by bacteria, viruses, and endotoxins that is likely facilitated by high constitutive expression of b3 integrin subunit (CD61), as well as TLR4, TLR9, and MRP-14, an endogenous activator of TLR (Parbhakar et al, 2004;Singh Suri et al, 2006;Jimenez-Marin et al, 2008;Schneberger et al, 2011bSchneberger et al, , 2012Duke-Novakovski et al, 2013). Because of the communication between the alveolar and capillary environments across the alveolar septum, activated pulmonary intravascular macrophages may amplify these signals resulting in higher levels of lung vascular inflammation (Schneberger et al, 2012).…”

Platelets in the Alzheimer’s Disease Brain: do they Play a Role in Cerebral Amyloid Angiopathy?

“…CD61 is also known as integrin β3 and is well established as a platelet marker. It is present as a subunit for the fibrinogen-receptor, playing a role in blood clotting [24]. CD62P is stored in alpha-granules of platelets, but normally is not expressed on the surface.…”