Molecular characterization and expression analysis of the IκB gene from pearl oyster Pinctada fucata

Zhang, Dianchang; Jiang, Shigui; Qiu, Lihua; Su, Tianfeng; K, Wu; Li, Youning; Zhu, Caiyan; Xu, Xinping

doi:10.1016/j.fsi.2008.10.009

Cited by 48 publications

(41 citation statements)

References 39 publications

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“…Our non-invasive experimental infection technique aimed at reproducing horizontal transfer of vibriosis to healthy oysters. For mollusk bivalves and C. gigas in particular, most studies reported in the literature have used a form of immune stimulation based on a treatment by inactivated microorganisms, mainly Micrococcus luteus, V. splendidus, V. anguillarum, V. alginolyticus, V. tubiashii, V. metschnikovii [16,23,48,49], or microbial components such as lipopolysaccharides poly I:C, poly G:C and dsDNA [48,50,51], with the main purpose of confirming the involvement of newly identified putative immune genes in defense reactions and studying them at a transcriptional level. These studies and some others used live bacteria, which were mostly harmless strains of Micrococcus luteus, V. alginolyticus, V. tasmaniensis, V. anguillarum, V. splendidus, V. tubiashii and V. metschnikovii [20,23,24,33,52,53].…”

Section: Discussionmentioning

confidence: 99%

Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes

Decker

Saulnier

2011

Fish & Shellfish Immunology

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The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP. No mortality occurred in control animals, but oysters put in cohabitation for 2-48 h with animals previously infected by two Vibrio pathogens suffered mortalities from 2 to 16 days post-cohabitation. Our results show that virulent Vibrio infect healthy individuals after only 2 h of cohabitation, with values ranging from 4.5 x 10² to 2 x 10⁴ cells ml⁻¹ hemolymph. Simultaneously, an approximate ten-fold increase of the total Vibrio population was observed in control animals, with a 6.6-78.5-fold up-expression of targeted genes. In contrast, oysters exposed to harmful bacteria had mean expression levels strongly down-regulated by a factor of 9.2-29 (depending on the gene) compared with control animals. Although oysters were still found to be infected by virulent Vibrio after 6-48 h of cohabitation, no significant differences were noted when comparing levels of each transcript in control and infected oysters at the same sampling times during this period: the important differences were noted before 6 h cohabitation. Taken together, our data support (1) the hypothesis that virulent Vibrio disturbs the immune response of this invertebrate host both rapidly and significantly, although this occurs specifically during an early and transient period during the first 6 h of cohabitation challenge, and that (2) expression of targeted genes is not correlated with vibriosis resistance.

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Section: Discussionmentioning

confidence: 99%

Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes

Decker

Saulnier

2011

Fish & Shellfish Immunology

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“…Our previous studies demonstrated that the expression level of LITAF and GILT of pearl oyster was significantly up-regulated after 8-h challenge (Zhang et al, 2009a(Zhang et al, ,b,c, 2010. Therefore, in this study, the 8-h time was selected for quantitative RT-PCR tissue analysis.…”

Section: Discussionmentioning

confidence: 97%

“…In order to control disease and enhance the yields and quality of seawater pearls, it is necessary to understand the innate immune defense mechanisms of pearl oyster, which lacks an adaptive immune system. Recently, we characterized some immune-related genes of pearl oyster, such as the inhibitor of NF-κB (IκB) (Zhang et al, 2009a), a clip-domain serine protease (Zhang et al, 2009b), a putative lipopolysaccharideinduced TNF-α factor (LITAF) (Zhang et al, 2009c), and interferon-gamma-inducible lysosomal thiol reductase (GILT) , and demonstrated that they played an important role in the innate immune responses of pearl oyster.…”

Section: Introductionmentioning

confidence: 99%

cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata

Hu²,

Hy³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Galectins can recognize and specifically bind to β-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. We cloned the cDNA of a tandem-repeat galectin from the pearl oyster Pinctada fucata (designated as PoGal2). PoGal2 cDNA is 1347 bp long and consists of a 5ꞌ-untranslated region (UTR) of 3 bp, a 3ꞌ-UTR of 297 bp with one cytokine RNA instability motif (ATTTA), and an open reading frame of 1047 bp, encoding a polypeptide of 349 amino acids, with an estimated molecular mass of 38.1 kDa and a theoretical isoelectric point of 8.5. PoGal2 contains two carbohydrate recognition domains (CRDs); both have the conserved carbohydrate-binding motifs H-NPR and WG-EE. PoGal2 shares 50.6 and 50.9% identity with those of abalone (Haliotis discus) and the Manila clam (Venerupis philippinarum), respectively. Phylogenetic analysis revealed that the tandem-repeat galectins formed two clades for the different species. Molluscan tandem-repeat galectins were clustered into a single clade, and nematode tandemrepeat galectins were clustered into another single clade. In both clades, CRD-N and CRD-C were divided into different groups. PoGal2 mRNA was constitutively expressed in all tissues analyzed, and the expression level of PoGal2 mRNA was found to be significantly up-regulated in digestive glands, gills and hemocytes after Vibrio alginolyticus stimulation/infection. Expression profile analysis showed that the expression level of PoGal2 mRNA was significantly up-regulated at 8, 12 and 24 h after V. alginolyticus infection. These results suggest that PoGal2 is a constitutive and inducible acute-phase protein involved in the innate immune response of pearl oysters.

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“…The bacterial challenge was carried out as described by Zhang [28] with the following modifications: in challenged group, pearl oysters were injected with 50 ml of live V. alginolyticus resuspended in PBS (pH 7.4, OD600 ¼ 0.4); control oysters were injected with 50 ml PBS. Haemolymph was collected at 0, 3, 6, and 12 h post challenge from the pericardial cavity using a 1 ml of syringe.…”

Section: Animal Culture and Bacterial Challengementioning

confidence: 99%

F-type lectin involved in defense against bacterial infection in the pearl oyster (Pinctada martensii)

Chen

Xiao

Zhou

2011

Fish & Shellfish Immunology

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Molecular characterization and expression analysis of the IκB gene from pearl oyster Pinctada fucata

Cited by 48 publications

References 39 publications

Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes

Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes

cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata

F-type lectin involved in defense against bacterial infection in the pearl oyster (Pinctada martensii)

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