Molecular Characterization and Evolutionary Analyses of Carnivore Protoparvovirus 1 NS1 Gene

Mira, Francesco; Canuti, Marta; Purpari, Giuseppa; Cannella, Vincenza; Occhiogrosso, Leonardo; Schirò, Giorgia; Chiaramonte, Gabriele; Barreca, Santino; Pisano, Patrizia; Decaro, Nicola; Guercio, Annalisa

doi:10.3390/v11040308

Cited by 36 publications

(50 citation statements)

References 72 publications

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“…Moreover, the evidence in the NS1/NS2 gene sequences of amino acid mutations with respect to the other CPV strains spreading worldwide (NS1: 60V, 544F, 545F, 630P ‐ NS2: 60V, 151N, 152V) could contribute to further elucidate its evolution (Mira et al, ).…”

Section: Resultsmentioning

confidence: 99%

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Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Mira

Purpari

Colaianni

et al. 2019

Transbound Emerg Dis

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Canine parvovirus type 2 (CPV‐2) emerged as dog pathogen in the late 1970s, causing severe and often fatal epizootics of gastroenteritis in the canine population worldwide. Although to date CPV‐2 is circulating in all continents, most of the current studies have analysed the amino acid changes accounted in the VP2 gene sequence, with limited information on virus introductions from other countries. The aim of this study was to analyse the genetic features of CPV‐2c strains currently spreading in Italy. Swabs and tissue samples were collected from dogs suspected of CPV infection. The nearly complete genome sequence from the CPV‐positive samples was obtained. The co‐circulation of two different but related CPV‐2c strains, with amino acid changes characteristic of CPV strains of Asian origin (NS1: 60V, 544F, 545F, 630P – NS2: 60V, 151N, 152V ‐ VP2: 5A/G, 267Y, 297A, 324I, 370R), were observed. The phylogenetic analyses inferred from the NS1 and VP2 gene sequences confirmed the relationship with Asian CPV‐2c strains. This study reports the spread of novel CPV‐2c mutants in Italy and supports further studies to evaluate the coexistence of genetically divergent CPV strains in the same geographical environment.

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Section: Resultsmentioning

confidence: 99%

“…Sequencing encompassing both ORFs (NS and VP genes) was carried out using primer pairs developed by Pérez et al (), as previously described (Mira et al, ). Sequences were assembled according to an overlapping strategy and analysed using BioEdit ver 7.0.5.3 software (Hall, ).…”

Section: Methodsmentioning

confidence: 99%

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Mira

Purpari

Colaianni

et al. 2019

Transbound Emerg Dis

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“…Limited studies are available on the CPV non‐structural genes (Hoelzer et al, ; Pérez et al, ), and, only recently, the analysis of the NS1 gene sequence was included in the CPV phylogenies from several countries (Canuti, Rodrigues, Whitney, & Lang, ; Grecco et al, ; Mira, Canuti, et al, ; Mira, Purpari, et al, ; Zhuang et al, ). In this study, sequence analysis revealed aa changes previously described mainly in NS1/NS2 gene sequences of CPV‐2a/2c strains of Asian origin.…”

Section: Discussionmentioning

confidence: 99%

“…This divergence may suggest the same ancestral origin with the CPV strains of Asian origin but a separate evolution, as well as a continuous adaptive process of the virus in separate environments. Indeed, some of these changes lay in the potential encoding sequence of functional domains (Mira, Canuti, et al, ) and, particularly, residues 351, 517 and 545 are located between the α5‐ and α6‐helices, between the β5‐ and α11‐helices and just close to the α11‐helix of the helicase domain protein sequence, respectively, as illustrated in Niskanen, Ihalainen, Kalliolinna, Häkkinen, and Vihinen‐Ranta (). Therefore, their role needs to be further evaluated.…”

Section: Discussionmentioning

confidence: 99%

“…Sequence analyses were carried out using primer pairs described by Pérez et al (), using the commercial kit GoTaq ® G2 DNA Polymerase (Promega Italia s.r.l. ), as previously described (Mira, Dowgier, et al, ) with minor modifications (Mira, Canuti, et al, ). After electrophoresis on agarose gel, amplicons were purified and submitted for direct Sanger sequencing with a set of primers, as previously described (Mira, Purpari, Lorusso, et al, ).…”

Section: Methodsmentioning

confidence: 99%

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Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Ogbu

Mira

Purpari

et al. 2019

Transbound Emerg Dis

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Canine parvovirus type 2 (CPV‐2) emerged suddenly in the late 1970s as pathogen of dogs, causing a severe and often fatal gastroenteric disease. The original CPV‐2 was replaced by three antigenic variants, CPV‐2a, CPV‐2b and CPV‐2c, which to date have gained a worldwide distribution with different relative proportions. All previous studies conducted in Africa were based on partial VP2 gene sequences. The aim of this study was to provide a genome analysis to characterize the CPV strains collected in Nigeria, Africa. Rectal swab samples (n = 320) were collected in 2018 and tested by means of an immunochromatographic assay. Among the 144 positive samples, 59 were selected for further analyses using different molecular assays. The results revealed a high prevalence of CPV‐2c (91.5%) compared to the CPV‐2a variant (8.5%). The VP2 gene sequences showed a divergence from the strains analysed in 2010 in Nigeria and a closer connection with CPV strains of Asian origin. The non‐structural gene analysis evidenced amino acid changes never previously reported. The molecular analysis based on genomic sequences evidenced a geographical pattern of distribution of the analysed strains, suggesting a potential common evolutionary origin with CPV of Asian origin. This study represents the first CPV molecular characterization including all the encoding gene sequences conducted in the African continent and contributes to define the current geographical spread of the CPV variants worldwide.

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Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population

Bui

Hoang

Dung³

et al. 2023

Veterinary Medicine & Sci

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Background: Canine parvovirus type 2 (CPV-2) is known as the primary etiological agent cause of acute gastroenteritis, myocarditis and death of canids worldwide. In Vietnam, although CPV-2 infection and its outbreaks are the most important risk factors of the canine's health concern, lack of available information about the molecular epidemiology of the CPV-2.Objectives: In this study, the complete coding sequences of 10 CPV-2 strains collected from dogs vaccinated with CPV-2 vaccination were analysed to better understand the genomic characteristics of the current circulating CPV-2 in Vietnam. Methods:Ten CPV-specific PCR-positive rectal swab samples were collected from dogs with acute symptoms of haemorrhagic diarrhoea and vomiting in Vietnam in 2019. The complete coding sequences of these CPV strains were analysed to determine their phylogeny and genetic relationship with other available CPV strains globally.Results: Analysis of the VP2 gene sequences demonstrated that the studied strains belonged to the new CPV-2c variants with the unique mutations at amino acids 5 Ala-Gly and 447 Iso-Met . Phylogenetic tree analysis indicated that the studied strains share a common evolutionary origin with the current CPV-2c strains circulating in dogs in Asia countries, including China, Thailand, Taiwan and Mongolia, in recent years. Low sequence identity between the studied strains and commercial vaccine strains was observed. Conclusions:This study provides deep insights into the molecular characteristics, genetic diversity, and evolution of circulating CPV-2 strains in Vietnam. We recommend more studies to estimate the effectiveness of the CPV vaccine and the need to continue developing other effective vaccination essential to better control the widespread of these new CPV-2 variants.

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Molecular Characterization and Evolutionary Analyses of Carnivore Protoparvovirus 1 NS1 Gene

Cited by 36 publications

References 72 publications

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population

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