Molecular characterization and evolution of a chemosensory receptor gene family in three notorious rice planthoppers, <scp>Nilaparvata lugens</scp>, <scp>Sogatella furcifera</scp> and <scp>Laodelphax striatellus</scp>, based on genome and transcriptome analyses

He, Peng; Engsontia, Patamarerk; Chen, Guanglei; Yin, Qian; Wang, Jun; Xu, Lixin; Zhang, Yanan; Li, Zhaoqun; He, Ming

doi:10.1002/ps.4912

Cited by 42 publications

(43 citation statements)

References 106 publications

(201 reference statements)

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“…(A)–(C)). Notably, all three rice planthoppers displayed a ‘lack of response,’ during which they did not select rice plants or an air control after 1.5 h. The results were similar to those observed with Orco deficit in rice planthoppers and OBP3 deficit in BPH, which revealed that rice planthoppers with a single OBP deficit indeed lose the ability to seek rice plants and exhibit delayed selection.…”

Section: Resultssupporting

confidence: 77%

“…(A)–(C)). The results were similar to the Orco RNAi efficacy in these three rice planthoppers, suggesting that RNAi is a practical tool to study the olfactory genes in rice planthoppers.…”

Section: Resultssupporting

confidence: 73%

“…8(A)-(C)). The results were similar to the Orco RNAi efficacy in these three rice planthoppers, 65 suggesting that RNAi is a practical tool to study the olfactory genes in rice planthoppers. We then conducted an olfactory response assay with 'H'-shaped olfactometers to determine whether a single OBP deficit in rice planthoppers compromises their ability to seek rice plants.…”

Section: Rnai and Relationships With Rice Plant-seeking Behaviorsupporting

confidence: 72%

“…The genomes of BPH, WBPH, and SBPH, and the head transcriptomes of WBPH and SBPH were used to identify novel candidate OBP unigenes based on a BLASTx algorithm‐based search using 12 known OBPs in WBPH, 10 OBPs in BPH, and 9 OBPs in SBPH (NCBI/GenBank database). We discovered two novel OBPs in BPH and seven novel OBPs in SBPH.…”

Section: Resultsmentioning

confidence: 99%

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Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus

Chen

et al. 2019

Pest Management Science

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BACKGROUND The white‐backed planthopper (WBPH) Sogatella furcifera, the brown planthopper (BPH) Nilaparvata lugens, and the small brown planthopper (SBPH) Laodelphax striatellus are three notorious rice pests that cause annual losses in rice yield through sap‐sucking and virus transmission. Odorant‐binding proteins (OBPs) are crucial olfactory genes involved in host‐seeking behavior. RESULTS We discovered the presence of 12, 12, and 16 OBPs in WBPH, BPH, and SBPH, respectively, including two novel OBPs in BPH and seven novel OBPs in SBPH. Phylogenetic analysis indicated that most of these OBPs have homologous genes, and one group (SfurOBP11, NlugOBP8, and LstrOBP2) show a slower evolution rate and are more conserved. Further, in vitro binding studies demonstrated that the three OBPs have similar binding affinities for some rice plant volatiles. Finally, RNA interference (RNAi) successfully inhibited the mRNA expression of the three OBPs, and in vivo behavioral tests showed that the OBP‐deficient rice planthoppers were partly anosmic and lost some of their ability to locate rice plants. CONCLUSION These results demonstrate the crucial role of the rice planthopper OBP genes in seeking rice plants. This information complements the current genetic resources for the development of RNAi‐based transgenic rice and other pest management technologies. © 2018 Society of Chemical Industry

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Section: Resultssupporting

confidence: 77%

Section: Resultssupporting

confidence: 73%

Section: Rnai and Relationships With Rice Plant-seeking Behaviorsupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

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Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus

Chen

et al. 2019

Pest Management Science

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“…Recently, 141, 135 and 37 ORs were separately identified from three rice planthoppers, Nilaparvata lugens, Sogatella furcifera and L. striatellus, in which Orco is the most conserved and essential odorant receptor gene. 41 The highly conserved Orco is considered as a potential target for a disruption of olfactory signaling. Excitingly, several N-, 2-substituted triazolothioacetamide compounds (VUAA1, VU0450667 and VU0183254) were recently reported to act directly on Orco subunit of Anopheles gambiae, 42,43 suggesting that broadly active compounds can be developed.…”

Section: Discussionmentioning

confidence: 99%

Olfactory co‐receptor Orco stimulated by Rice stripe virus is essential for host seeking behavior in small brown planthopper

Zhou

2018

Pest Management Science

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Silencing of Decapentaplegic (Dpp) gene inhibited the wing expansion in the white‐backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

Long

Yang

Jin

et al. 2022

Arch Insect Biochem Physiol

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The Decapentaplegic gene controls wing patterning and spreading by regulating downstream genes in many insect species. However, the molecular characteristics, expression, and biological function of Dpp in Sogatella furcifera remain poorly understood. In this study, we cloned the Dpp gene from S. furcifera and examined its expression levels in different development stages, wing typed adults, and tissues. Then, the function of SfDpp gene was analyzed using an RNA interference (RNAi)‐based approach. The results showed that the full‐length complementary DNA of the SfDpp gene consists of 1034 bp and contains a 954‐bp open reading frame encoding 317 amino acids. SfDpp has a transforming growth factor‐β (TGF‐β) propeptide superfamily domain and a TGF‐β superfamily domain, typical of members of the TGF‐β superfamily. Quantitative real‐time polymerase chain reaction showed that the expression of SfDpp reached its highest expression level 40 min after eclosion. RNAi‐based gene silencing inhibited transcript levels of the corresponding messenger RNA in S. furcifera nymphs injected with double‐stranded RNA of SfDpp and resulted in death of 29.17% and 26.67% of 4th and 5th instar nymphs, respectively. The wing deformity rate of the adults was 74.12% and 3.41% after SfDpp gene silencing in 4th and 5th instar nymphs, respectively. Examining wing development‐associated genes showed that two target genes of Dpp (Vestigial and Spalt) were both dramatically downregulated after SfDpp was silenced. Our results demonstrate that downregulated SfDpp in early development causes wing expansion failure in S. furcifera. Thus, Dpp may be a target gene for restricting the migration of rice‐damaging planthoppers.

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Molecular characterization and evolution of a chemosensory receptor gene family in three notorious rice planthoppers, Nilaparvata lugens, Sogatella furcifera and Laodelphax striatellus, based on genome and transcriptome analyses

Abstract: The results demonstrate the importance of the planthopper Orco genes in locating rice plants. This information may aid in the development of RNAi-based transgenic rice and other pest management technologies. © 2018 Society of Chemical Industry.

Cited by 42 publications

References 106 publications

Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus

Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus

Olfactory co‐receptor Orco stimulated by Rice stripe virus is essential for host seeking behavior in small brown planthopper

Silencing of Decapentaplegic (Dpp) gene inhibited the wing expansion in the white‐backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

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