Molecular characterization and elucidation of the function of Hap38 MAPK in the response of Helicoverpa armigera (Hübner) to UV-A stress

Yao, Mengshuang; Liu, Xiaofei; Meng, Jian‐Yu; Yang, Chang-Li; Zhang, Changyu

doi:10.1038/s41598-022-23363-x

Cited by 2 publications

(3 citation statements)

References 37 publications

(38 reference statements)

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“…Insect MAPKs have attracted the attention of many researchers because of their highly conserved and critical functions in development, immunity, and stress responses. In lepidopteran insects, Hap38 MAPK from H. armigera (Hübner) was cloned, and its function in response to UV‐A stress was elucidated (Yao et al, 2022). The p38 MAPK, MEK, and ERK cDNA were cloned from silkworm B. mori , and sequence analysis showed that their amino acid sequences were highly similar to those of other species, in which ERK phosphorylation plays a vital role in the resumption of development and diapause termination (Fujiwara et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…ERK signaling links nutrient status to antiviral roles in the insect gut (Xu et al, 2013). The p38 MAPK pathway protects against environmental stresses and Bt Cry toxins (Cancino‐Rodezno et al, 2010; Yao et al, 2022). In the present study, Cry1Ab upregulated the expression of OfAKHR at 12 and 24 h, then suppressed the expression at 48 h posttreatment in O. furnacalis larvae; the expression of OfAKHR was significantly upregulated after the knockdown of OfERK , which indicated a potential negative regulation relationship.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that Cs-ERK2 is involved in response to Bt infection (Qiu, Zhang, et al, 2017). Therefore, we postulated that OfERK has a potential function in defense against (Hübner) was cloned, and its function in response to UV-A stress was elucidated (Yao et al, 2022). The p38 MAPK, MEK, and ERK cDNA were cloned from silkworm B. mori, and sequence analysis showed that their amino acid sequences were highly similar to those of other species, in which ERK phosphorylation plays a vital role in the resumption of development and diapause termination (Fujiwara et al, 2006).…”

Section: The Regulation Of Oferk On Immune- Developmental-and Stress-...mentioning

confidence: 99%

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Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

Li,

Xu,

Zhang

et al. 2023

Arch Insect Biochem Physiol

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The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.43%–98.08% amino acid identities with other insect mitogen‐activated protein kinases. For spatiotemporal analysis of the expression pattern, OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. Moreover, the results demonstrated that the Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression after Cry1Ab oral treatment. The expression of OfERK was downregulated through RNA interference, and the correlation of its expression with other related genes was verified using quantitative real‐time polymerase chain reaction. Our study provides valuable insights into the regulatory mechanism of ERK in insects for future studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Regulation Of Oferk On Immune- Developmental-and Stress-...mentioning

confidence: 99%

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Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

Li,

Xu,

Zhang

et al. 2023

Arch Insect Biochem Physiol

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Effect of ultraviolet on the environmental adaptability of Megalurothrips usitatus (Thysanoptera: Thripidae)

Jin,

Wang,

Jiang

et al. 2024

Arch Insect Biochem Physiol

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Megalurothrips usitatus Bagnall, an important pest of bean plants, is primarily managed with synthetic insecticides. M. usitatus has developed considerable resistance to various insecticides in multiple cowpea‐growing areas in Hainan Province, China, posing challenges to its control in the field. Light control technology is a potentially effective physical control method for M. usitatus. The vision of thrips is highly sensitive to UV light, whereas other biological characteristics remain unknown. Therefore, this study evaluated the effects of ultraviolet light on the biological characteristics of M. usitatus. Results showed that the egg, larval, and pupal stages of M. usitatus were significantly shortened, and the emergence rate (79.59%) and adult survival rate (77.95%) were reduced under a devoid of UV light environment (UV−), compared with the full‐spectrum light (control treatment group, CK) (p < 0.05). However, the single spawning quantity and total amount of spawning were significantly higher, and the sex ratio (57%) was the highest under UV− (p < 0.05). Single UV light (UV+) only affected the pupation rate. Also, the antioxidant enzymes, polyphenol oxidase, superoxide dismutase (SOD), and peroxidase activities were significantly and negatively correlated with the progression of generations under UV−, whereas catalase and SOD activities were significantly and positively correlated with the progression of generations under UV+. The UV− light conditions significantly interfered with the behavior selection of M. usitatus. The results of this study showed that the adaptability of M. usitatus populations would be greatly reduced in the absence of ultraviolet light, providing a theoretical basis for the control of M. usitatus populations.

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Molecular characterization and elucidation of the function of Hap38 MAPK in the response of Helicoverpa armigera (Hübner) to UV-A stress

Cited by 2 publications

References 37 publications

Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

Effect of ultraviolet on the environmental adaptability of Megalurothrips usitatus (Thysanoptera: Thripidae)

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