Molecular Characterization and DNA Fingerprinting of Xanthomonas oryzae pv. oryzae Isolates from Climate Change Prone Areas in East Africa

Genetic Analysis and Molecular Identification of Virulence in Xanthomonas oryzae pv. oryzae Isolates

“…Molecular typing using random amplified polymorphic (RAPD) markers has revealed two major ( Mta and Mtb ) virulence genotypes among the 50 Xoo isolates in which Mta was virulence ( Vr ) and Mtb mildly virulence ( MVr ). This paper supports recent isozyme fingerprints of 30 Xoo isolates from 5 countries (Mali, Burkina Faso, Niger, Benin Republic, and Nigeria) in West Africa and molecular analysis of 25 Xoo isolates from East Africa that revealed two major genetic groups [ 10 , 11 , 26 ]. These two genotypes of Xoo virulence identified by molecular typing were very identical to Xoo virulence pathotypes ( Pta and Ptb ) obtained using phenotypic pathotyping indicating possible linkage and correlation between phenotypic pathotyping and molecular typing of Xoo virulence [ 25 , 27 ].…”

“…To understand the epidemiology and ecology of Xoo pathogens and their potential for virulence change, various phenotypic characters as well as molecular markers have been used in studies of Xoo pathogen population structure [ 3 , 4 , 10 , 11 ]. Rapid identification and classification of bacteria are normally carried out by morphology, nutritional requirements, antibiotic resistance, isozyme comparisons, phage sensitivity [ 7 , 10 , 12 ], and more recently DNA based methods, particularly rRNA sequences [ 13 , 14 ], strain-specific fluorescent oligonucleotides [ 15 ], and the polymerase chain reaction (PCR) [ 12 , 16 ]. Several repetitive elements found in the Xoo pathogen have been used as probes in restriction fragment length polymorphism (RFLP) analysis [ 17 ].…”