Molecular characterization and distribution of the voltage-gated sodium channel, Para, in the brain of the grasshopper and vinegar fly

Wang, Hongxia; Foquet, Bert; Dewell, Richard B.; Song, Ho‐Jun; Dierick, Herman A.; Gabbiani, Fabrizio

doi:10.1007/s00359-019-01396-4

Cited by 8 publications

(13 citation statements)

References 104 publications

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“…Sample collection, RNA extraction and RNA quality assessment were performed as previously described (Wang et al, 2020). Briefly, crowded-reared and isolated-reared female nymphs were marked with a ceramic marker on the abdomen after molting to their last nymphal instar, and were dissected around 72 h later.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…Both RNA sequencing and transcriptome assembly used for the present study were previously described in detail in Wang et al (2020). Briefly, we generated RNA-seq data by performing paired-end sequencing (150 bp) on 8.5 lanes on an Illumina HiSeq4000 (San Diego, CA).…”

Section: Rna Sequencing and Transcriptome Assemblymentioning

confidence: 99%

“…Sequence information can be found in Table 1. Additionally, mapping reads were counted as previously described (Wang et al, 2020), using bowtie2 (very sensitive end-to-end, disable no-mixed and no-discordant behavior) and SAMtools' idxstats (Li et al, 2009) in Galaxy. Differential expression analysis was performed with DEseq2 (Love, Huber & Anders, 2014) within SARTools 1.7.1 (Varet et al, 2016), using R 3.5.3 (R Core Team, 2017).…”

Section: Sequence Assembly and Expression Analysismentioning

confidence: 99%

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There is no magic bullet: the importance of testing reference gene stability in RT-qPCR experiments across multiple closely related species

Foquet

Song

2020

PeerJ

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Reverse Transcriptase quantitative Polymerase Chain Reaction (RT-qPCR) is the current gold standard tool for the study of gene expression. This technique is highly dependent on the validation of reference genes, which exhibit stable expression levels among experimental conditions. Often, reference genes are assumed to be stable a priori without a rigorous test of gene stability. However, such an oversight can easily lead to misinterpreting expression levels of target genes if the references genes are in fact not stable across experimental conditions. Even though most gene expression studies focus on just one species, comparative studies of gene expression among closely related species can be very informative from an evolutionary perspective. In our study, we have attempted to find stable reference genes for four closely related species of grasshoppers (Orthoptera: Acrididae) that together exhibit a spectrum of density-dependent phenotypic plasticity. Gene stability was assessed for eight reference genes in two tissues, two experimental conditions and all four species. We observed clear differences in the stability ranking of these reference genes, both between tissues and between species. Additionally, the choice of reference genes clearly influenced the results of a gene expression experiment. We offer suggestions for the use of reference genes in further studies using these four species, which should be taken as a cautionary tale for future studies involving RT-qPCR in a comparative framework.

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Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Section: Rna Sequencing and Transcriptome Assemblymentioning

confidence: 99%

Section: Sequence Assembly and Expression Analysismentioning

confidence: 99%

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There is no magic bullet: the importance of testing reference gene stability in RT-qPCR experiments across multiple closely related species

Foquet

Song

2020

PeerJ

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“…Thus, various qualities are used for the identification and pursuit of conspecifics and prey, for the escape from predators, for monitoring self-movement, for maintaining orientation in space, and on a more basic level, for the entrainment of internal circadian clocks. The neuronal machinery evaluating the inputs from the eyes can be enormous and, in many species, encompasses more than three quarters of all brain neurons (Strausfeld 1976). In insects and crustaceans, primary visual processing takes place in a sequence of optic neuropils, termed lamina, medulla, and lobula complex that are retinotopically organized into layers and columns.…”

mentioning

confidence: 99%

“…A comprehensive understanding of visual circuits not only requires identifying the contributing neuronal cell types, but also an understanding of synaptic mechanisms, neurotransmitters and the cell type specific expression of ion channels. Two contributions toward these directions are provided by Gür et al (2020) and Wang et al (2020). Gür et al (2020) analyzed the basis of membrane properties of lamina monopolar neurons at the first stage of processing in the motion vision system of Drosophila.…”

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confidence: 99%

Visual circuits in arthropod brains

Homberg

2020

J Comp Physiol A

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Comparative analysis of phenotypic plasticity sheds light on the evolution and molecular underpinnings of locust phase polyphenism

Foquet

Castellanos

Song

2021

Sci Rep

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Locusts exhibit one of nature’s most spectacular examples of complex phenotypic plasticity, in which changes in density cause solitary and cryptic individuals to transform into gregarious and conspicuous locusts forming large migrating swarms. We investigated how these coordinated alternative phenotypes might have evolved by studying the Central American locust and three closely related non-swarming grasshoppers in a comparative framework. By experimentally isolating and crowding during nymphal development, we induced density-dependent phenotypic plasticity and quantified the resulting behavioural, morphological, and molecular reaction norms. All four species exhibited clear plasticity, but the individual reaction norms varied among species and showed different magnitudes. Transcriptomic responses were species-specific, but density-responsive genes were functionally similar across species. There were modules of co-expressed genes that were highly correlated with plastic reaction norms, revealing a potential molecular basis of density-dependent phenotypic plasticity. These findings collectively highlight the importance of studying multiple reaction norms from a comparative perspective.

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Molecular characterization and distribution of the voltage-gated sodium channel, Para, in the brain of the grasshopper and vinegar fly

Cited by 8 publications

References 104 publications

There is no magic bullet: the importance of testing reference gene stability in RT-qPCR experiments across multiple closely related species

There is no magic bullet: the importance of testing reference gene stability in RT-qPCR experiments across multiple closely related species

Visual circuits in arthropod brains

Comparative analysis of phenotypic plasticity sheds light on the evolution and molecular underpinnings of locust phase polyphenism

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