Molecular characterization and corrosion behavior of thermophilic (55 °C) SRB <i>Desulfotomaculum kuznetsovii</i> isolated from cooling tower in petroleum refinery

Anandkumar, B.; Choi, Jeong‐Hee; Venkatachari, G.; Maruthamuthu, S.

doi:10.1002/maco.200805177

Cited by 23 publications

(7 citation statements)

References 42 publications

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“…Instead, after 28 d, members of the family Peptococcaceae (a family within the Firmicutes), including Desulfotomaculum spp., were dominant, with acetate oxidation coupled with Fe(III) and sulfate reduction ( Fig 5A, 5C and 5E ). Several species in the genus Desulfotomaculum are known to reduce sulfate, coupled with oxidation of acetate to CO 2 via an acetyl-CoA pathway [ 45 – 49 ]. The observed consumption of ~5 mM acetate and ~4 mM sulfate was in good agreement with the stoichiometry for sulfate reduction coupled with acetate oxidation (CH 3 COO − + SO 4 2− → 2HCO 3 − + HS − ), sulfide and bicarbonate being likely products, but not directly observed.…”

Section: Resultsmentioning

confidence: 99%

Impact of Organic Carbon Electron Donors on Microbial Community Development under Iron- and Sulfate-Reducing Conditions

et al. 2016

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Although iron- and sulfate-reducing bacteria in subsurface environments have crucial roles in biogeochemical cycling of C, Fe, and S, how specific electron donors impact the compositional structure and activity of native iron- and/or sulfate-reducing communities is largely unknown. To understand this better, we created bicarbonate-buffered batch systems in duplicate with three different electron donors (acetate, lactate, or glucose) paired with ferrihydrite and sulfate as the electron acceptors and inoculated them with subsurface sediment as the microbial inoculum. Sulfate and ferrihydrite reduction occurred simultaneously and were faster with lactate than with acetate. 16S rRNA-based sequence analysis of the communities over time revealed that Desulfotomaculum was the major driver for sulfate reduction coupled with propionate oxidation in lactate-amended incubations. The reduction of sulfate resulted in sulfide production and subsequent abiotic reduction of ferrihydrite. In contrast, glucose promoted faster reduction of ferrihydrite, but without reduction of sulfate. Interestingly, the glucose-amended incubations led to two different biogeochemical trajectories among replicate bottles that resulted in distinct coloration (white and brown). The two outcomes in geochemical evolution might be due to the stochastic evolution of the microbial communities or subtle differences in the initial composition of the fermenting microbial community and its development via the use of different glucose fermentation pathways available within the community. Synchrotron-based x-ray analysis indicated that siderite and amorphous Fe(II) were formed in the replicate bottles with glucose, while ferrous sulfide and vivianite were formed with lactate or acetate. These data sets reveal that use of different C utilization pathways projects significant changes in microbial community composition over time that uniquely impact both the geochemistry and mineralogy of subsurface environments.

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Section: Resultsmentioning

confidence: 99%

Impact of Organic Carbon Electron Donors on Microbial Community Development under Iron- and Sulfate-Reducing Conditions

et al. 2016

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“…It plays an important role in MIC ( Cetin et al, 2007 ) by accelerating cathodic depolarization and decelerating anodic depolarization ( Cetin and Aksu, 2009 ). The ability of members of this genus to corrode steel has been studied extensively in laboratory experiments: D. nigrificans ( Mystkowska et al, 2015 ), D. orientis ( Ren and Wood, 2004 ), and D. kuznetsovii ( Anandkumar et al, 2015 ). Members of this genus are usually associated with oil, and have been isolated from the crude oil field, oil production wells, or even the cooling towers of a petroleum refinery ( Cetin et al, 2007 ; Cetin and Aksu, 2009 ; Anandkumar et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing

Duan

Xiao

et al. 2017

Front. Microbiol.

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Metal corrosion is of worldwide concern because it is the cause of major economic losses, and because it creates significant safety issues. The mechanism of the corrosion process, as influenced by bacteria, has been studied extensively. However, the bacterial communities that create the biofilms that form on metals are complicated, and have not been well studied. This is why we sought to analyze the composition of bacterial communities living on steel structures, together with the influence of ecological factors on these communities. The corrosion samples were collected from rust layers on steel plates that were immersed in seawater for two different periods at Sanya and Xiamen, China. We analyzed the bacterial communities on the samples by targeted 16S rRNA gene (V3–V4 region) sequencing using the Illumina MiSeq. Phylogenetic analysis revealed that the bacteria fell into 13 phylotypes (similarity level = 97%). Proteobacteria, Firmicutes and Bacteroidetes were the dominant phyla, accounting for 88.84% of the total. Deltaproteobacteria, Clostridia and Gammaproteobacteria were the dominant classes, and accounted for 70.90% of the total. Desulfovibrio spp., Desulfobacter spp. and Desulfotomaculum spp. were the dominant genera and accounted for 45.87% of the total. These genera are sulfate-reducing bacteria that are known to corrode steel. Bacterial diversity on the 6 months immersion samples was much higher than that of the samples that had been immersed for 8 years (P < 0.001, Student’s t-test). The average complexity of the biofilms from the 8-years immersion samples from Sanya was greater than those from Xiamen, but not significantly so (P > 0.05, Student’s t-test). Overall, the data showed that the rust layers on the steel plates carried many bacterial species. The bacterial community composition was influenced by the immersion time. The results of our study will be of benefit to the further studies of bacterial corrosion mechanisms and corrosion resistance.

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“…It was observed that the biofilm formation initiates after lapse of a certain incubation period and that the corrosion products (iron sulfides) start affecting the biofilm after a certain incubation period. Anandkumar et al [18]; Anandkumar and Rajeshkar, [19], investigated the role of Desulfotomaculum geothermicum and Desulfotomaculum kuznetsovii in mild steel corrosion. The presence of bacteria enhances corrosion by accelerating cathodic reaction and suppressing anodic reaction.…”

Section: Introductionmentioning

confidence: 99%

Microbial influenced corrosion by thermophilic bacteria

2011

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The present study was undertaken to investigate microbial influenced corrosion (MIC) on stainless steels due to thermophilic bacteria Desulfotomaculum nigrificans. The objective of the study was to measure the extent of corrosion and correlate it with the growth of the biofilm by monitoring the composition of its extracellular polymeric substances (EPS). The toxic effect of heavy metals on MIC was also observed. For this purpose, stainless steels 304L, 316L and 2205 were subjected to electrochemical polarization and immersion tests in the modified Baar's media, control and inoculated, in anaerobic conditions at room temperature. Scanning electron microscopy (SEM)/ energy dispersive spectroscopy (EDS) were used to identify the chemicals present in/outside the pit. The results show maximum corrosive conditions when bacterial activity is highest, which in turn minimizes the amount of carbohydrate and protein along with the increase in the fraction of uronic acid in carbohydrate in EPS of the biofilm. However, although bacterial activity and corrosion rate decreases, the amount of biofilm components continue to increase. It is also observed that the toxicity of metals ions affect the bacterial activity and EPS production. It was observed that Desulfotomaculum sp. has the ability to biodegrade its own EPS.

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Molecular characterization and corrosion behavior of thermophilic (55 °C) SRB Desulfotomaculum kuznetsovii isolated from cooling tower in petroleum refinery

Cited by 23 publications

References 42 publications

Impact of Organic Carbon Electron Donors on Microbial Community Development under Iron- and Sulfate-Reducing Conditions

Impact of Organic Carbon Electron Donors on Microbial Community Development under Iron- and Sulfate-Reducing Conditions

Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing

Microbial influenced corrosion by thermophilic bacteria

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