Molecular characterization and comparative expression analysis of two teleostean pro-inflammatory cytokines, IL-1β and IL-8, from Sebastes schlegeli

Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis

“…The predicted pmIL-8 monomeric 3D structure was composed by three anti-parallel b-strands and a single a-helix at the C-terminus region, which is suggested to be involved in the formation of the IL-8 receptor binding site, and is consistent with the known IL-8 structure of other teleosts and human (Clore et al, 1990;Wang et al, 2013;Herath et al, 2016). Another particular common feature of the tertiary .…”

“…Furthermore, the lack of knowledge regarding immune responses in P. mesopotamicus prevents the development of appropriate treatments and prophylactic strategies to decrease the frequency in which these outbreaks occur (Farias et al, 2018). bacterial and parasitic infections, and the analysis of their expression is recognized as good indicators of the occurrence of inflammatory processes (Morrison, 2012;Herath et al, 2016;Wang et al, 2017;Wang et al, 2019). Recently, these cytokines have also been recognized as potential molecular adjuvants in fish vaccination, with promising results already obtained against viral and bacterial pathogens (Peddie et al, 2003;Jimenez et al, 2006;Sanchez, 2007;Wang et al, 2016;Wang et al, 2019), further increasing the importance of studies providing their structural and functional characterization.…”

“…In mammalian IL-8, a three-amino acid residue motif (Glu-Leu-Arg) is commonly found preceding the first cysteine (ELR + CXC). The presence of this motif confers to proteins the ability to exert a chemoattractant effect on leukocytes, as well as to promote angiogenesis (Allen et al, 2007;Harun et al, 2008;Herath et al, 2016). In teleost fish, however, this motif has not been identified in any species, with the exception being haddock (Melanogrammus aeglefinus), which has led to the speculation of whether fish IL-8 might have fewer functions than their mammalian counterparts, a hypothesis that is still not confirmed (Seppola et al, 2008).…”

RNA-Seq analysis of salinity stress–responsive transcriptome in the liver of spotted sea bass (Lateolabrax maculatus)

“…Similarity, in Asian seabass ( Latescal carifer ), complement C1q-like protein 2-like showed significant down-regulation after high salinity challenge [ 18 ]. Interleukin-8, a major pro-inflammatory cytokines which play a vital role in initiation of inflammatory responses against bacterial- and viral-infections [ 58 ], also showed differentially regulated between LS and HS groups ( Table 4 ). Interleukin-8 was also observed appearing on the DEGs list of Asian seabass after long-time salinity challenge [ 18 ].…”

Identificação e expressão gênica das enzimas arginase 1, arginase 2, e citocinas pró-inflamatórias em <i>Pseudoplatystoma corruscans</i> e <i>Pseudoplatystoma reticulatum</i>, imunizados com <i>Ichthyophthirius multifiliis</i>

“…). Mais especificamente a IL-8 é considera um quimiocina devido a sua participação nos movimentos quimiotáticos em reações inflamatórias(HERATH et al, 2016). Esta quimiocina é pertencentes a um grupo de pequenas proteínas que possuem de 8-12 KDa contendo um domínio de resíduo CXC conservado (C-cisteína, X-qualquer outro resíduo) proximal ao seuN-terminal (ALLEN;CROWN;HANDEL, 2007).…”