Molecular characterization and biological functioning of interleukin-8 in Siberian sturgeon (Acipenser baeri)

Wang, Xiaowen; Ma, Guoying; Zhang, Rong; Liu, Lili; Zhu, Jianya; Zhu, Hua

doi:10.1016/j.fsi.2019.04.010

Cited by 29 publications

(21 citation statements)

References 46 publications

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“…Based on magnitude of fold-change, Gram-negative bacterium (i.e., E. tarda ) and LPS, which is a structural constituent of Gram-negative strains, had a greater impact on Ofcxcl8-L1 expression compared with Gram-positive bacterium (i.e., S. iniae ) suggesting that Gram-negative bacteria appear to be the potent stimulant of Ofcxcl8-L1 transcription. This fact corroborates with the previous findings reported in different fish-pathogen models, including mrigal against Aeromonas hydrophila / E. tarda [ 85 ], grass carp against A. hydrophila [ 18 ], turbot against E. tarda [ 21 ], Siberian sturgeon against A. hydrophila [ 31 ] and large yellow croaker against Vibrio parahaemolyticus [ 22 ] infections.…”

Section: Discussionsupporting

confidence: 92%

“…In contrast to tetrapod CXCL8s, as found in many other reported teleostean CXCL8s [ 70 ], OfCXCL8s possessed ELR motif variants, EMH and NSH tripeptide sequences in OfCXCL8-L1 and OfCXCL8-L3, respectively. Among all the teleost CXCL8s characterized, homologs with the ELR motif have only been reported in haddock [ 71 ], Atlantic cod [ 34 ] and Siberian sturgeon [ 31 ]. However, several mutagenesis studies showed that the modification of ELR motif had no significant impact on the chemotactic activity of CXCL8 in different fish species [ 21 , 30 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, CXCL8-L3 is a recently identified lineage, which has been reported in large yellow croaker [ 26 ] and rainbow trout [ 11 ]. While the majority of the previous studies were primarily focused on transcriptional responses of cxcl8 [ 12 , 17 , 22 , 27 , 28 ], some groups have also examined the biological functions of fish CXCL8, such as chemotaxis [ 11 , 13 , 14 , 15 , 18 , 19 , 20 , 21 , 23 , 26 , 29 , 30 , 31 , 32 , 33 ], cell proliferation [ 20 , 21 , 31 ], superoxide production/respiratory burst [ 15 , 22 , 23 , 26 ], and phagocytosis [ 30 , 32 ]. Carp recombinant CXCL8s, CXCa-L1 and CXCL8-L2, demonstrated chemotactic activity both in vivo and in vitro, and enhanced the respiratory burst [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses

et al. 2020

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CXCL8 (interleukin-8, IL-8) is a CXC family chemokine that recruits specific target cells and mediates inflammation and wound healing. This study reports the identification and characterization of two cxcl8 homologs from rock bream, Oplegnathus fasciatus. Investigation of molecular signature, homology, phylogeny, and gene structure suggested that they belonged to lineages 1 (L1) and 3 (L3), and designated Ofcxcl8-L1 and Ofcxcl8-L3. While Ofcxcl8-L1 and Ofcxcl8-L3 revealed quadripartite and tripartite organization, in place of the mammalian ELR (Glu-Leu-Arg) motif, their peptides harbored EMH (Glu-Met-His) and NSH (Asn-Ser-His) motifs, respectively. Transcripts of Ofcxcl8s were constitutively detected by Quantitative Real-Time PCR (qPCR) in 11 tissues examined, however, at different levels. Ofcxcl8-L1 transcript robustly responded to treatments with stimulants, such as flagellin, concanavalin A, lipopolysaccharide, and poly(I:C), and pathogens, including Edwardsiella tarda, Streptococcus iniae, and rock bream iridovirus, when compared with Ofcxcl8-L3 mRNA. The differences in the putative promoter features may partly explain the differential transcriptional modulation of Ofcxcl8s. Purified recombinant OfCXCL8 (rOfCXCL8) proteins were used in in vitro chemotaxis and proliferation assays. Despite the lack of ELR motif, both rOfCXCL8s exhibited leukocyte chemotactic and proliferative functions, where the potency of rOfCXCL8-L1 was robust and significant compared to that of rOfCXCL8-L3. The results, taken together, are indicative of the crucial importance of Ofcxcl8s in inflammatory responses and immunoregulatory roles in rock bream immunity.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses

et al. 2020

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“…Supplementation with βG increased IL‐8 and diminished IgM, SOD and CAT gene expression due to ATZ. Accordingly, βG ameliorated inflammation and encouraged the immune response (Dawood et al, ; Wang et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…IgM is often used as a measure of fish humoral adaptive immunity (Chen et al, 2013). Interleukin-8 is a small cytokine that acts as a fundamental factor in inflammatory reactions (Wang et al, 2019). Exposure to ATZ encourages immunosuppression and inflammatory reactions (Toughan et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

2020

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Three hundred Oreochromis niloticus were divided into five groups; the control fish (CNT) were fed a basal diet, beta glucan (βG) group was fed 0.5 g/kg−1 βG, atrazine (ATZ) group was exposed to 1/5 96‐hr LC50 (1.39 mg/L) ATZ, the (βG/ATZ) group was fed βG while being exposed to ATZ, and the (βG then ATZ) group was supplemented with βG for fifteen days before exposed to ATZ. ATZ exposure caused a decline in growth that was ameliorated by βG. ATZ reduced the levels of total and different types of leucocytes. Additionally, ATZ exposure caused reductions in total proteins, globulins, α 1‐globulin, α 2‐globulin, ɤ‐globulin, immunoglobulin M, lysozymes, superoxide dismutase, nitric oxide and catalase but increases in hepatic transaminases and malondialdehyde without any variations in albumin and β‐globulin. Exposure to ATZ also resulted in a rise in the mRNA level of IL‐8. In contrast, expression of IgM, SOD and CAT were decreased in the tilapias exposed to ATZ. Exposure to ATZ increases the susceptibility response to Aeromonas sobria challenge, as indicated by an increase in cumulative mortality post‐challenge. Supplementation with βG fifteen days before (βG then ATZ group), counteracted the adverse effects of ATZ on the immune, biochemical and antioxidants values, though only slight alleviation was observed with simultaneous treatment (βG/ATZ group). Our results established that ATZ has adverse impacts on immune responses, antioxidant equilibrium and its related genes. While, supplementation with βG before exposure to ATZ may be valuable for counteracting the possible damage caused by ATZ water pollution than its simultaneous treatment with ATZ.

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Advances in chemokines of teleost fish species

Liu

2024

Aquaculture and Fisheries

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Molecular characterization and biological functioning of interleukin-8 in Siberian sturgeon (Acipenser baeri)

Cited by 29 publications

References 46 publications

Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses

Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

Advances in chemokines of teleost fish species

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