Molecular characterization and antimicrobial susceptibility testing of clinical and non-clinical Brucella melitensis and Brucella abortus isolates from Egypt

Wareth, Gamal; El-Diasty, Mohamed; Abdel‐Hamid, Nour H.; Holzer, Katharina; Hamdy, Mahmoud; Moustafa, Shawky; Shahein, Momtaz A.; Melzer, Falk; Beyer, Wolfgang; Pletz, Mathias W.; Neubauer, Heinrich

doi:10.1016/j.onehlt.2021.100255

Cited by 29 publications

(28 citation statements)

References 43 publications

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“…As crocodile lizards are a protected species, we chose closely related Chinese skinks ( Plestiodon chinensis ) for in vivo testing and constructed an artificial infection animal model by creating abrasions on the skin of the skinks ( 14 , 16 , 45 – 48 ). Jiang et al ( 14 ) also used Chinese skinks as an animal model for cutaneous granuloma identification in crocodile lizards.…”

Section: Methodsmentioning

confidence: 99%

Identification of Pseudomonas aeruginosa From the Skin Ulcer Disease of Crocodile Lizards (Shinisaurus crocodilurus) and Probiotics as the Control Measure

Xiong

Qin

et al. 2022

Front. Vet. Sci.

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The crocodile lizard (Shinisaurus crocodilurus) is an endangered ancient reptile species. Captive breeding is an important conservation measure for the potential restoration and recovery of their wild populations. However, a skin ulcer disease caused by an unknown pathogen has become a serious threat to captive breeding individuals. In the current study, based on microbial isolation, we identified Pseudomonas aeruginosa as the dominant pathogen in skin ulcer disease. Chinese skinks (Plestiodon chinensis) were used to verify the pathogenicity of P. aeruginosa in skin ulcer disease in vivo. As expected, subcutaneous inoculation of P. aeruginosa induced skin disease in healthy skinks and P. aeruginosa was re-isolated from the induced skin ulcers. Therefore, P. aeruginosa, an opportunistic and ubiquitous pathogen that causes a wide range of infections, appears to be the main pathogen of the skin disease affecting crocodile lizards. In the aquaculture industry, probiotics are widely used in the prevention and control of animal diseases caused by such pathogens. Here, we administered probiotics to the breeding crocodile lizards for 6 months. The three experiment groups treated with different kinds of probiotics showed significance at controlling case incidence. Three of the four groups treated with probiotics showed significant disease prevention (Effective Microorganisms mixed probiotics P = 0.0374; Double-dose Effective Microorganisms, P = 0.0299; Bacillus subtilis, P = 0.0140, T-test), and CFUs in the water of the breeding enclosures were also inhibited after probiotics usage (P < 0.001, T-test). Our study demonstrated the role of Pseudomonas aeruginosa in development of skin ulcer disease of crocodile lizards in a local zoo and offered the probiotic-based method for control measurements, which would be of benefit for the conservation of endangered reptiles.

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Section: Methodsmentioning

confidence: 99%

Identification of Pseudomonas aeruginosa From the Skin Ulcer Disease of Crocodile Lizards (Shinisaurus crocodilurus) and Probiotics as the Control Measure

Xiong

Qin

et al. 2022

Front. Vet. Sci.

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“…Viewpoint, serology, and nucleic acid firming examinations are three distinct categories used in the bacteriological judgment of humanoid brucellosis (NAATs). This analysis presents a computation of the comparative benefits in addition to downsides of the diagnostic techniques' current ranking, medical use, and new improvements [21,22].…”

Section: Diagnosis Of Brucellosismentioning

confidence: 99%

Overview of Brucellosis: Simple Review Article

Alharbi¹,

Alanazi²,

Alanazi³

et al. 2022

Pharmacophore

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In the majority of the developing ecosphere, brucellosis is an endemic zoonotic disease that has devastating effects on the livestock industry and small-scale cattle owners. Clinical symptoms displayed by infected animals have an economic impact on stakeholders. These include a significant decline in milk manufacturing, reduced fertility, abortion, poor weight gain, losses in thet drawing power, and poor weight gain. Brucellosis commonly presents in people as a range of non-specific clinical symptoms. Brucella infections can damage any organ or bodily system and lead to a range of focal issues and clinical situations, despite the fact that brucellosis in humans often presents as a feverish condition with no obvious focus, either from the outset of the disease or during its course. Unfortunately, only a small number of these potential settings-primarily infections brought on by B. melitensis-have received enough research into the diagnostic yield of NAATs. Due to these restrictions, we will concentrate on the clinical conditions with the strongest body of data.

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“…The disease is highly contagious and of significant concern to farmers due to potential economic losses. Treatment of animals is not recommended while humans are treated with different antibiotics combinations [58].…”

Section: Brucella Abortusmentioning

confidence: 99%

Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens

Świętnicki

2021

Biomolecules

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Bacterial secretory systems are essential for virulence in human pathogens. The systems have become a target of alternative antibacterial strategies based on small molecules and antibodies. Strategies to use components of the systems to design prophylactics have been less publicized despite vaccines being the preferred solution to dealing with bacterial infections. In the current review, strategies to design vaccines against selected pathogens are presented and connected to the biology of the system. The examples are given for Y. pestis, S. enterica, B. anthracis, S. flexneri, and other human pathogens, and discussed in terms of effectiveness and long-term protection.

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Molecular characterization and antimicrobial susceptibility testing of clinical and non-clinical Brucella melitensis and Brucella abortus isolates from Egypt

Cited by 29 publications

References 43 publications

Identification of Pseudomonas aeruginosa From the Skin Ulcer Disease of Crocodile Lizards (Shinisaurus crocodilurus) and Probiotics as the Control Measure

Identification of Pseudomonas aeruginosa From the Skin Ulcer Disease of Crocodile Lizards (Shinisaurus crocodilurus) and Probiotics as the Control Measure

Overview of Brucellosis: Simple Review Article

Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens

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