Molecular characterization and antigenic properties of a novel Babesia gibsoni glutamic acid-rich protein (BgGARP)

Mousa, Ahmed Abdelmoniem; Cao, Shinuo; Aboge, Gabriel Oluga; Terkawi, Mohamad Alaa; Kirdasy, Ahmed El; Salama, Akram; Attia, Mabrouk; AbouLaila, Mahmoud; Zhou, Mo; Kamyingkird, Ketsarin; Moumouni, Paul Franck Adjou; Masatani, Tatsunori; Aziz, Samy A. Abd El; Moussa, Waheed Mohammed; Chahan, Bayin; Fukumoto, Shinya; Nishikawa, Yoshifumi; Ballal, Salah Sayed El; Xuan, Xuenan

doi:10.1016/j.exppara.2013.08.005

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…The human immune response is primarily directed to R2 (aa 705-1178), where the highly immunogenic repeats are located, and this region of GLURP has been developed primarily as a blood-stage vaccine (38). In addition to Plasmodium, many other pathogens, such as Babesia gibsoni (56), Entamoeba histolytica (57), Pneumocystis carinii, and Mycobacterium tuberculosis (58), also have immunogenic glutamate-rich proteins, and some of them were evaluated as new diagnostic tools or vaccine candidates. Additionally, many allergens from plants (59)(60)(61) and some self-antigens that lead to autoimmune disease (62,63) were also glutamate-rich antigens.…”

Section: Discussionmentioning

confidence: 99%

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Hou

Jiang

et al. 2020

Front. Immunol.

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Induction of humoural immunity is critical for clinical protection against malaria. More than 100 malaria vaccine candidates have been investigated at different developmental stages, but with limited protection. One of the roadblocks constrains the development of malaria vaccines is the poor immunogenicity of the antigens. The objective of this study was to map the linear B-cell epitopes of the Plasmodium falciparum erythrocyte invasion-associated antigens with a purpose of understanding humoural responses and protection. We conducted a large-scale screen using overlapping peptide microarrays of 37 proteins from the P. falciparum parasite, most of which are invasion-associated antigens which have been tested in clinical settings as vaccine candidates, with sera from individuals with various infection episodes. Analysis of the epitome of the antigens revealed that the most immunogenic epitopes were predominantly located in the low-complexity regions of the proteins containing repetitive and/or glutamate-rich motifs in different sequence contexts. However, in vitro assay showed the antibodies specific for these epitopes did not show invasion inhibitory effect. These discoveries indicated that the low-complexity regions of the parasite proteins might drive immune responses away from functional domains, which may be an instructive finding for the rational design of vaccine candidates.

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Section: Discussionmentioning

confidence: 99%

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Hou

Jiang

et al. 2020

Front. Immunol.

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“…To date, EWAS (and related approaches) have reported specific DNA methylation changes in association with Type II diabetes , body mass index , autoimmune disorders, including Type I diabetes and adverse pregnancy outcomes (among other phenotypes). Interestingly, genes identified in EWAS as differentially methylated in association with preeclampsia differ according to tissue sampled, platform utilized and population assessed . Therefore, although revealing potential pathways to disease and biomarkers for disease tracking, such findings need to be interpreted with an appropriate degree of caution until a higher degree of consensus is achieved.…”

Section: Association Of Epigenetic Change With Human Diseasementioning

confidence: 99%

Epigenetics as the mediator of fetal programming of adult onset disease: what is the evidence?

Saffery

Novakovic

2014

Acta Obstet Gynecol Scand

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The Developmental Origins of Health and Disease hypothesis describes how early life environmental factors influence development in a way that impacts later health and disease risk. The hypothesis is supported by a large number of animal studies and a smaller number of observational studies in humans. Epigenetic variation induced in early life has emerged as a prime candidate to be the mediator of such effects, but little direct evidence of this relation exists in humans, primarily due to the inherent problems associated with unraveling the relative contributions of genetic and environmental variables to phenotypic diversity. There are several prerequisites for establishing a causal link that include demonstrating interindividual epigenetic variability in early life in response to specific environmental exposures. Further, compelling evidence linking epigenetic change to disease, prior to onset is required. Finally, the functional relevance of specific epigenetic change must be demonstrated. Evidence is emerging in all of these areas but, ultimately, only large longitudinal life-course studies, commencing prior to birth, can provide direct evidence in support of a role of epigenetic processes as a driver of Developmental Origins of Health and Disease in humans.

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“…A number of potent antigens have been isolated using this immunoscreening technique, and their diagnostic and vaccine potentials subsequently demonstrated (Table 1) [34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57]. In parallel to the immunoscreening strategy, expressed sequence tag (EST) databases can be used to isolate and identify novel genes for developmental purposes.…”

Section: Identification Of Genes Encoding New Proteinsmentioning

confidence: 99%

New Molecules inBabesia gibsoni and Their Application for Diagnosis, Vaccine Development, and Drug Discovery

Goo

Xuan

2014

Korean J Parasitol

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Babesia gibsoni is an intraerythrocytic apicomplexan parasite that causes piroplasmosis in dogs. B. gibsoni infection is characterized clinically by fever, regenerative anemia, splenomegaly, and sometimes death. Since no vaccine is available, rapid and accurate diagnosis and prompt treatment of infected animals are required to control this disease. Over the past decade, several candidate molecules have been identified using biomolecular techniques in the authors' laboratory for the development of a serodiagnostic method, vaccine, and drug for B. gibsoni. This review article describes newly identified candidate molecules and their applications for diagnosis, vaccine production, and drug development of B. gibsoni.

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Molecular characterization and antigenic properties of a novel Babesia gibsoni glutamic acid-rich protein (BgGARP)

Cited by 6 publications

References 20 publications

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Epigenetics as the mediator of fetal programming of adult onset disease: what is the evidence?

New Molecules inBabesia gibsoni and Their Application for Diagnosis, Vaccine Development, and Drug Discovery

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