Molecular characterization and Antibiotic resistance profiles of Escherichia coli extended-spectrum β-lactamases producer strains isolated from urine samples in Benin

Abstract

Urinary tract infections are the second common reason of medical consultations and antibiotics prescription. Escherichia coli is known to cause most urinary tract infections. The aim of this study was to characterize and determine the antibiotic resistance profile of E. coli extended-spectrum βlactamases (ESBL) producer strains isolated from urine samples. The urine samples collected came from hospitalized and non-hospitalized patient referred to Hubert Koutoukou Manga (HKM), National and University Hospital Center (Cotonou, Benin). The resistance to antibiotics was determined according to the disk diffusion method. The production of penicillinase and ESBLs was researched respectively by the acidimetric test and double disk synergy method. The presences of genes encoding βlactamases were detected by Polymerase Chain Reaction (PCR). Our data revealed that 60 % of E. coli strains (101) were isolated from female patients. Also, 69.31 % of the strains were isolated from non-hospitalized patients. The high resistance levels were recorded with amoxicillin (96.04 %) and amoxicillin + clavulanic acid (66.34 %). Twenty percent (20%) of strains were ESBLs. Among ESBLs strains, 70% comes from non-hospitalized patients. Eighty percent of E. coli strains produced penicillinase among which 25 % were ESBL producers. All the ESBL producers strains carried blaTEM gene whereas only 30 % carried the blaSHV gene. This study updates the data on the prevalence to antibiotic resistance of E. coli ESBL producers strains for better management of urinary tract infections.

Molecular characterization of beta-lactamase genes produced by community-acquired uropathogenic Escherichia coli in Nouna

“…As for ESBL-encoding genes, they consisted of bla CTX-M-1 group ESBL in 92.3% (12/13) of our ESBLproducing isolates. This frequency of CTX-M-1 type ESBL is similar to the one found previously in Burkina Faso (94%), by Ouedraogo et al, 2016 [15] and it is congruent with the report of the same author [9] that CTX-M-15 is the most frequent ESBL found in West-Africa, as well as in many other parts of the world [3,4,20,21].…”

“…The migration of PCR products from our ESBL strains demonstrated the production of two types of narrow-spectrum beta-lactamases: the TEM (53.8%), and OXA (61.5%). This is lower than a previous report by Mousse et al, 2018 [20] where 100% of TEM type beta-lactamase were found in a similar study of ESBL E. coli genes isolated from urine samples in Benin. Additionally, Kpoda et al, 2018 [21] found an overall 24.6% prevalence of TEM encoding genes in E. coli isolates obtained from urine, pus, blood, stool, vaginal swab and pleural fluid samples of patients attending three major hospitals in the capital of Burkina Faso, which indicates a variability in the distribution of these genes among E. coli in Africa.…”