Molecular Characteristics of Disease-Causing and Commensal Staphylococcus lugdunensis Isolates from 2003 to 2013 at a Tertiary Hospital in Taiwan

Yeh, Chun‐Fu; Liu, Tsui-Ping; Cheng, Chun-Wen; Chang, Shih-Cheng; Lee, Ming-Hsun; Lu, Jang‐Jih

doi:10.1371/journal.pone.0134859

Cited by 17 publications

(21 citation statements)

References 39 publications

(44 reference statements)

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confidence: 60%

“…agr typing. We carried out agr typing by multiplex PCR amplification of the conserved sequences of the agrC gene with two forward primers (5=-CTGTCATCCTTAGTGTAATTGCTG-3= and 5=-GCCGGCATAAT AGTCCCTTCTG-3=) and one reverse primer (5=-CGAACCTTTAGCTT ATCTGTACC-3=), as previously described (14). We determined the agr specificity type of the isolates on the basis of the expected product sizes (586 bp for agr type I and 771 bp for agr type II).…”

Section: Methodsmentioning

confidence: 99%

“…Staphylococcal cassette chromosome mec element (SCCmec) type V was the most common SCCmec type. We also found that the proportion of SCCmec type II isolates was higher for commensal isolates than for isolates causing disease in health care settings (13,14).Multilocus sequence typing (MLST) characterizes multilocus genetic variability on the basis of the sequences of multiple housekeeping genes (15). MLST has been used for population genetic analyses and studies of phylogenetic structure and global epidemiology in many microorganisms.…”

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Clinical Features, Outcomes, and Molecular Characteristics of Community- and Health Care-Associated Staphylococcus lugdunensis Infections

et al. 2016

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Staphylococcus lugdunensis is a major cause of aggressive endocarditis, but it is also responsible for a broad spectrum of infections. The differences in clinical and molecular characteristics between community-associated (CA) and health care-associated (HA) S. lugdunensis infections have remained unclear. We performed a retrospective study of S. lugdunensis infections between 2003 and 2014 to compare the clinical and molecular characteristics of CA and HA isolates. We collected 129 S. lugdunensis isolates in total: 81 (62.8%) HA isolates and 48 (37.2%) CA isolates. HA infections were more frequent than CA infections in children (16.0% versus 4.2%, respectively; P ‫؍‬ 0.041) and the elderly (38.3% versus 14.6%, respectively; P ‫؍‬ 0.004). The CA isolates were more likely to cause skin and soft tissue infections (85.4% versus 19.8%, respectively; P < 0.001). HA isolates were more frequently responsible for bacteremia of unknown origin (34.6% versus 4.2%, respectively; P < 0.001) and for catheter-related bacteremia (12.3% versus 0%, respectively; P ‫؍‬ 0.011) than CA isolates. Fourteen-day mortality was higher for HA infections than for CA infections (11.1% versus 0%, respectively). A higher proportion of the HA isolates than of the CA isolates were resistant to penicillin (76.5% versus 52.1%, respectively; P ‫؍‬ 0.004) and oxacillin (32.1% versus 2.1%, respectively; P < 0.001). Two major clonal complexes (CC1 and CC3) were identified. Sequence type 41 (ST41) was the most common sequence type identified (29.5%). The proportion of ST38 isolates was higher for HA than for CA infections (33.3% versus 12.5%, respectively; P ‫؍‬ 0.009). These isolates were of staphylococcal cassette chromosome mec element (SCCmec)type IV, V, or Vt. HA and CA S. lugdunensis infections differ in terms of their clinical features, outcome, antibiotic susceptibilities, and molecular characteristics. S taphylococcus lugdunensis was first described by Freney et al. in 1988 (1). It has emerged as an important human pathogen in recent years, due to its ability to cause endocarditis and a broad spectrum of other infections, including skin and soft tissue infections, bloodstream infections, bone and joint infections, central nervous system infections, and intra-abdominal infections (2).S. lugdunensis has a lower incidence than Staphylococcus aureus and Staphylococcus epidermidis (3-5). Many studies have focused on cases of bacteremia and endocarditis caused by S. lugdunensis and have shown that this bacterium can, like S. aureus, cause invasive infections but with higher rates of complications and mortality (4, 6-10). Endocarditis due to this bacterium has been reported to be mostly community associated (CA) (4, 7). Two retrospective studies showed that most CA S. lugdunensis infections were skin and soft tissue infections, with a total absence of oxacillin resistance (11, 12). We recently reported 48 cases of invasive S. lugdunensis infection. Most of these cases (43 of 48 [89.6%]) were health care-associated (HA) infections, and the frequency ...

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Clinical Features, Outcomes, and Molecular Characteristics of Community- and Health Care-Associated Staphylococcus lugdunensis Infections

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“…lugdunensis isolates, both commensal and infectious from a Twaiwanese tertiary hospital over a 10-year window from 2003 to 2013, found that the agr types occurred with roughly similar frequencies and did not show any statistically significant correlation with infection type. 30 …”

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Truncated Autoinducing Peptides as Antagonists of Staphylococcus lugdunensis Quorum Sensing

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Competitive quorum sensing antagonisim (QS) offers a novel strategy for attenuating current multidrug resistant staphylococcal infections. To this end, a series of 10 truncated analogues based on the parent autoinducing peptides (AIPs) of Staphylococcus lugdunensis (groups I and II) and Staphylococcus epidermidis (groups I–III) were sequentially assessed against a newly developed Staphylococcus lugdunensis group I QS reporter strain. The truncated analogues based upon Staphylococcus lugdunensis AIP-1 (1) and AIP-2 (2) displayed respective IC50 values of 0.2 ± 0.01 μM and 0.3 ± 0.01 μM, while the truncated analogue of the Staphylococcus epidermidis AIP-1 (3) elicited an IC50 value of 2.7 ± 0.1 μM. These findings demonstrate the potential of cognate and “crosstalk” competitive quorum sensing inhibition using truncated AIPs as a means of attenuating staphylococcal infections in species beyond Staphylococcus aureus.

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“…While antibiotic resistance in S. lugdunensis is not regarded as a major clinical problem, resistance to aminoglycosides, macrolides, oxacillin, and tetracycline have been increasingly reported (3)(4)(5). In dialysis settings, topical mupirocin has been shown to substantially reduce the risk for S. aureus infections (6).…”

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Emergence of ileS2 -Carrying, Multidrug-Resistant Plasmids in Staphylococcus lugdunensis

Liu

Chow

et al. 2016

Antimicrob Agents Chemother

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dOf 137 Staphylococcus lugdunensis isolates collected from two nephrology centers in Hong Kong, 10 (7.3%) and 3 (2.2%) isolates had high-level and low-level mupirocin resistance, respectively. Isolates with high-level resistance contained the plasmid-mediated ileS2 gene, while isolates with low-level resistance contained the mutation V588F within the chromosomal ileS gene. All but one of the ileS2-positive isolates belong to the predominating clone HKU1. Plasmids carrying the ileS2 gene were mosaic and also cocarry multiple other resistance determinants.

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Molecular Characteristics of Disease-Causing and Commensal Staphylococcus lugdunensis Isolates from 2003 to 2013 at a Tertiary Hospital in Taiwan

Cited by 17 publications

References 39 publications

Clinical Features, Outcomes, and Molecular Characteristics of Community- and Health Care-Associated Staphylococcus lugdunensis Infections

Clinical Features, Outcomes, and Molecular Characteristics of Community- and Health Care-Associated Staphylococcus lugdunensis Infections

Truncated Autoinducing Peptides as Antagonists of Staphylococcus lugdunensis Quorum Sensing

Emergence of ileS2 -Carrying, Multidrug-Resistant Plasmids in Staphylococcus lugdunensis

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