Molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China

Sun, Chen; Wang, Qing; Li, Wenting; Wen, Denian; Chen, Chang-Hui; Yang, Xin; Shi, Wei; Meng, Qinghong; Yao, Kaihu; Qian, Suyun

doi:10.1007/s12519-019-00317-4

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…Although our study was not designed or powered as an epidemiological survey, this collection is probably representative of the current epidemiology in this part of the world, as it shows spa-types that are widely distributed in Asia 12 or, more specifically in China, 13 with spa-type t189 being more common among MSSA and spa-type t437, among MRSA, as previously described. 14 ST59-t437-agr group I, which was recently reported as the most frequent genotype in MRSA isolated from hospitalized children in China, 15 was also predominant here. More globally, agr group I is also highly prevalent in MSSA in China, 16 which is also what we observed here.…”

Section: Discussionsupporting

confidence: 64%

Antibiotic Resistance, Biofilm Formation, and Intracellular Survival As Possible Determinants of Persistent or Recurrent Infections by Staphylococcus aureus in a Vietnamese Tertiary Hospital: Focus on Bacterial Response to Moxifloxacin

Nguyen

Argudín

Deplano

et al. 2020

Microbial Drug Resistance

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Resistance is notoriously high in Asia but may not entirely explain therapeutic failures. Specific modes of bacterial life, such as biofilm or intracellular survival, may also contribute to the persistent and/or recurrent character of infections. Most Staphylococcus aureus isolates form biofilm and many survive and even thrive intracellularly. We collected 36 non-duplicate S. aureus isolates (including 18 MRSA) from patients with clinical evidence of persistent or recurrent infections in a large tertiary Vietnamese hospital. We examined their antibiotic resistance profile (MIC determination) and clonal relatedness (spa and agr typing, PFGE profiles). We then assessed the activity of moxifloxacin in both biofilms and infected phagocytes (moxifloxacin previously proved to be one of the most active antibiotics against reference strains in these models). spa-types t189 and t437 and agr group I were the most frequent. 30/36 isolates were multidrug resistant but 30/36 were recovered from patients having received an active drug. All tested isolates produced biofilm and survived inside phagocytes. At its human Cmax, moxifloxacin was inactive on biofilms made by moxifloxacin-susceptible as well as moxifloxacin-resistant isolates. It caused only modest intracellular CFU decrease against moxifloxacin-susceptible isolates and was inactive against those resistant to moxifloxacin. While our data confirm for this

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Section: Discussionsupporting

confidence: 64%

Antibiotic Resistance, Biofilm Formation, and Intracellular Survival As Possible Determinants of Persistent or Recurrent Infections by Staphylococcus aureus in a Vietnamese Tertiary Hospital: Focus on Bacterial Response to Moxifloxacin

Nguyen

Argudín

Deplano

et al. 2020

Microbial Drug Resistance

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“…Our multi-centre MRSA molecular surveillance schemes, together with work from Xiao et al 2013 which considered different administrative regions, previously found that hospital-associated ST239 was the dominant MRSA lineage in China between 2005 and 2011, accounting for 50-80.8% of MRSA [6][7][8]37], but has subsequently been declining in frequency (4.3%), with a general replacement of HA-MRSA STs by CA-MRSA STs [38]. CA-MRSA lineages, including ST59, have been increasing in prevalence since 2013 [24,[38][39][40], with recent work suggesting that between 2015 and 2017, ST59 has become the predominant MRSA clone (33%, 154/471) with ST59 accounting for more than half of CA-MRSA isolates [41]. The replacement of ST239 has been observed in other countries [42][43][44].…”

mentioning

confidence: 96%

“…ST8 (USA300) is prevalent in the USA but is rarely observed in the rest of the world [16,19]. ST22 is the most common lineage in the UK [15,16,20,21] and has been identified at differing prevalence in parts of Asia [22][23][24].…”

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confidence: 99%

Drivers of methicillin-resistant Staphylococcus aureus (MRSA) lineage replacement in China

et al. 2021

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Background Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen subdivided into lineages termed sequence types (STs). Since the 1950s, successive waves of STs have appeared and replaced previously dominant lineages. One such event has been occurring in China since 2013, with community-associated (CA-MRSA) strains including ST59 largely replacing the previously dominant healthcare-associated (HA-MRSA) ST239. We previously showed that ST59 isolates tend to have a competitive advantage in growth experiments against ST239. However, the underlying genomic and phenotypic drivers of this replacement event are unclear. Methods Here, we investigated the replacement of ST239 using whole-genome sequencing data from 204 ST239 and ST59 isolates collected in Chinese hospitals between 1994 and 2016. We reconstructed the evolutionary history of each ST and considered two non-mutually exclusive hypotheses for ST59 replacing ST239: antimicrobial resistance (AMR) profile and/or ability to colonise and persist in the environment through biofilm formation. We also investigated the differences in cytolytic activity, linked to higher virulence, between STs. We performed an association study using the presence and absence of accessory virulence genes. Results ST59 isolates carried fewer AMR genes than ST239 and showed no evidence of evolving towards higher AMR. Biofilm production was marginally higher in ST59 overall, though this effect was not consistent across sub-lineages so is unlikely to be a sole driver of replacement. Consistent with previous observations of higher virulence in CA-MRSA STs, we observed that ST59 isolates exhibit significantly higher cytolytic activity than ST239 isolates, despite carrying on average fewer putative virulence genes. Our association study identified the chemotaxis inhibitory protein (chp) as a strong candidate for involvement in the increased virulence potential of ST59. We experimentally validated the role of chp in increasing the virulence potential of ST59 by creating Δchp knockout mutants, confirming that ST59 can carry chp without a measurable impact on fitness. Conclusions Our results suggest that the ongoing replacement of ST239 by ST59 in China is not associated to higher AMR carriage or biofilm production. However, the increase in ST59 prevalence is concerning since it is linked to a higher potential for virulence, aided by the carriage of the chp gene.

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“…Among numerous clinical lineages, sequence type (ST) 59 has become the predominant community-associated methicillin-resistant S. aureus (CA-MRSA) in Aisa, and research in the southwest of China has found that seb was the most prevalent in the MRSA ST59 isolates, which implies a significant role and high prevalence of Staphylococcal enterotoxin B (SEB). 2 , 3 …”

Section: Introductionmentioning

confidence: 99%

A highly neutralizing human monoclonal antibody targeting a novel linear epitope on staphylococcal enterotoxin B

Fan,

Zhao,

Wang

et al. 2024

Human Vaccines & Immunotherapeutics

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Molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China

Cited by 8 publications

References 38 publications

Antibiotic Resistance, Biofilm Formation, and Intracellular Survival As Possible Determinants of Persistent or Recurrent Infections by Staphylococcus aureus in a Vietnamese Tertiary Hospital: Focus on Bacterial Response to Moxifloxacin

Antibiotic Resistance, Biofilm Formation, and Intracellular Survival As Possible Determinants of Persistent or Recurrent Infections by Staphylococcus aureus in a Vietnamese Tertiary Hospital: Focus on Bacterial Response to Moxifloxacin

Drivers of methicillin-resistant Staphylococcus aureus (MRSA) lineage replacement in China

A highly neutralizing human monoclonal antibody targeting a novel linear epitope on staphylococcal enterotoxin B

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