Molecular characterisation of two <i>α</i>‐esterase genes involving chlorpyrifos detoxification in the diamondback moth, <i>Plutella xylostella</i>

Xie, Ming; Ren, Na; You, Yunxiang; Chen, Weijun; Song, Qisheng; You, Minsheng

doi:10.1002/ps.4445

Cited by 34 publications

(28 citation statements)

References 48 publications

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“…Our result is consistent with a previous study that showed knockdown of esterase genes in Bactrocera dorsalis also increased the sensitivity of fruit flies to malathion (Wang et al, 2016(Wang et al, , 2017. In P. xylostella, decreased expression level of EST gene by RNAi could also significantly increase the sensitivity of larvae to the insecticide chlorpyrifos (Xie et al, 2017). In malathion-resistant Locusta migratoria, knockdown of carboxylesterases via RNAi would significantly increased susceptibility to malathion treatment (Zhang et al, 2013).…”

Section: Discussionsupporting

confidence: 92%

“…Many studies have revealed that multiple overexpressed EST genes produce more protein to detoxify insecticides (Jackson et al, 2013;Yang, 2016;Xie et al, 2017;Zhang et al, 2018; The synergism ratio (SR) indicated that LC 50 value without synergist was divided by LC 50 value with synergist. Synergists such as triphenyl phosphate (TPP), diethyl maleate (DEM), and S, S, S-tributyl phosphorotrithioate (DEF) can specifically inhibit carboxylesterases, GST and esterase, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

et al. 2020

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Esterases (ESTs) play important roles in metabolizing various physiologically endogenous and exogenous compounds, and various environmental xenobiotics in insects. The psocid, Liposcelis bostrychophila is a major pest of stored products worldwide and rapidly develops resistance to commonly insecticides. However, the involvement of ESTs in insecticide metabolization and the application of RNAi approach in psocids have not been well elucidated. In this study, we characterized four LbEST genes and investigated the transcriptional levels of these genes at different developmental stages and under different insecticides exposures to assess their potential roles in response to insecticides. The four LbESTs contain a catalytic triad (Ser-His-Glu) linked to an oxyanion hole and acyl pocket involved in substrate stabilization during its hydrolysis. Synergism observed with the esterase-inhibitor DEF suggests the involvement of esterases in malathion detoxification. LbESTs were expressed during the whole of developmental stages, but predominant abundance in the first nymphal instar and adult stage. The mRNA level of three LbEST genes (except for LbEST4) was induced (1.29-to 5.60 fold) in response to malathion or deltamethrin exposures, indicating that these esterases are involved in the detoxification process. Silencing of LbEST1, LbEST2 or LbEST3 through dsRNA feeding led to a higher mortality of psocids upon the malathion treatment compared to controls (1.83 to 2.69-fold), demonstrating that these esterase genes play roles in malathion detoxification in L. bostrychophila. Our study provides new evidence for understanding of the function and regulation mechanism of esterases in L. bostrychophila in insecticide detoxification. The current study also suggests that the present RNAi method could be applied for gene functional studies in psocids.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

et al. 2020

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“…Enzymatic detoxification is identified as a metabolic resistance process that involves the biochemical transformation of insecticides and further increases the biodegradation of toxic insecticides . This generally occurs due to the continuously increased expression and/or induction and elevated activity of members of three detoxification enzyme families: cytochrome P450 proteins (CYPs), carboxylesterases (CarEs), and glutathione S‐transferases (GSTs) . It has been shown that CYPs are the primary enzyme family that is associated with resistance to chemical insecticides in insect species .…”

Section: Introductionmentioning

confidence: 99%

“…2,11 This generally occurs due to the continuously increased expression and/or induction and elevated activity of members of three detoxification enzyme families: cytochrome P450 proteins (CYPs), carboxylesterases (CarEs), and glutathione S-transferases (GSTs). [13][14][15] It has been shown that CYPs are the primary enzyme family that is associated with resistance to chemical insecticides in insect species. 2,11 To date, a total of 143 CYPs, which can be assigned to four clans (clan 4, clan 3, clan 2, and a mitochondrial clan), have been identified in T. castaneum.…”

Section: Introductionmentioning

confidence: 99%

CYP4BN6 and CYP6BQ11 mediate insecticide susceptibility and their expression is regulated by Latrophilin in Tribolium castaneum

Xiong

Gao

Mao

et al. 2019

Pest Management Science

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BACKGROUND Many insect cytochrome P450 proteins (CYPs) are involved in the metabolic detoxification of exogenous compounds such as plant toxins and insecticides. Tribolium castaneum, the red flour beetle, is a major agricultural pest that damages stored grains and cereal products. With the completion of the sequencing of its genome, two T. castaneum species‐specific CYP genes, CYP4BN6, and CYP6BQ11, were identified. However, it is unknown whether the functions of most CYPs are shared by TcCYP4BN6 and TcCYP6BQ11, and the upstream regulatory mechanism of these two CYPs remains elusive. RESULTS QRT‐PCR analysis indicated that TcCYP4BN6 and TcCYP6BQ11 were both most highly expressed at the late pupal stage and were mainly observed in the head and gut, respectively, of adults. Moreover, the transcripts of these two CYPs were significantly induced by dichlorvos and carbofuran, and RNA interference (RNAi) targeting of each of them enhanced the susceptibility of beetles to these two insecticides. Intriguingly, knockdown of the latrophilin (lph) gene, which has been reported to be related to the insecticide susceptibility, reduced the expression of TcCYP4BN6 and TcCYP6BQ11 after insecticide treatment, suggesting that these two CYP genes are regulated by lph to participate in insecticide susceptibility in T. castaneum. CONCLUSION These results shed new light on the function and mechanism of CYP genes associated with insecticide susceptibility and could facilitate research on appropriate and sustainable pest control management. © 2019 Society of Chemical Industry

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“…The diamondback moth is highly productive with a short lifecycle and it is able to migrate long distances (Li, Feng, Liu, You, & Furlong, ). More importantly, the diamondback moth can acquire high levels of resistance against pesticides and Bacillus thuringiensis (Bt) toxins (Troczka, Williamson, Field, & Davies, ; Xie et al., ; Zhang et al., ). Therefore, the study of signaling molecules in the Imd pathway may help to uncover potential targets for the biological control of the diamondback moth.…”

Section: Introductionmentioning

confidence: 99%

Cloning and analysis of peptidoglycan recognition protein‐LC and immune deficiency from the diamondback moth, Plutella xylostella

Zhan

Yang

Rao

2017

Arch Insect Biochem Physiol

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Peptidoglycan (PGN) exists in both Gram-negative and Gram-positive bacteria as a component of the cell wall. PGN is an important target to be recognized by the innate immune system of animals. PGN recognition proteins (PGRP) are responsible for recognizing PGNs. In Drosophila melanogaster, PGRP-LC and IMD (immune deficiency) are critical for activating the Imd pathway. Here, we report the cloning and analysis of PGRP-LC and IMD (PxPGRP-LC and PxIMD) from diamondback moth, Plutella xylostella (L.), the insect pest of cruciferous vegetables. PxPGRP-LC gene consists of six exons encoding a polypeptide of 308 amino acid residues with a transmembrane region and a PGRP domain. PxIMD cDNA encodes a polypeptide of 251 amino acid residues with a death domain. Sequence comparisons indicate that they are characteristic of Drosophila PGRP-LC and IMD homologs. PxPGRP-LC and PxIMD were expressed in various tissues and developmental stages. Their mRNA levels were affected by bacterial challenges. The PGRP domain of PxPGRP-LC lacks key residues for the amidase activity, but it can recognize two types of PGNs. Overexpression of full-length and deletion mutants in Drosophila S2 cells induced expression of some antimicrobial peptide genes. These results indicate that PxPGRP-LC and PxIMD may be involved in the immune signaling of P. xylostella. This study provides a foundation for further studies of the immune system of P. xylostella.

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Molecular characterisation of two α‐esterase genes involving chlorpyrifos detoxification in the diamondback moth, Plutella xylostella

Abstract: RNAi knockdown of Pxae18 and Pxae28 significantly inhibited detoxification ability and increased the mortality in P. xylostella. The results indicate that these two CarE genes play important roles in the detoxification of chlorpyrifos in P. xylostella. © 2016 Society of Chemical Industry.

Cited by 34 publications

References 48 publications

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

CYP4BN6 and CYP6BQ11 mediate insecticide susceptibility and their expression is regulated by Latrophilin in Tribolium castaneum

Cloning and analysis of peptidoglycan recognition protein‐LC and immune deficiency from the diamondback moth, Plutella xylostella

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