Molecular characterisation of nerol dehydrogenase gene (PmNeDH) from Persicaria minor in response to stress-related phytohormones

Abstract

Citral is a mixture of neral and geranial, which are of great interest to the fragrance industry due to its lemon-scented aroma. A newly characterized nerol dehydrogenase of Persicaria minor (PmNeDH) from our recent findings has shown a capacity to convert citral from nerol. Differential gene expression analysis revealed that the expression level of PmNeDH was highly upregulated during early treatment of several stress-related phytohormones i.e. methyl jasmonate (MeJA), salicylic acid (SA) and abscisic acid (ABA). SA and ABA were shown to have a prolonged effect on PmNeDH expression level until second day of treatment. The findings were in agreement with the cisregulatory elements predicted from the gene promoter. The phylogenetic relationship of PmNeDH with its homologs from the medium-chain dehydrogenases/reductases (MDR) superfamily was also mentioned. In this study, we proposed a possible biological function of PmNeDH gene in P. minor, which might play significant roles in plant defence mechanism.

Isolation, purification and biochemical characterization of Conopomorpha cramerella farnesol dehydrogenase

“…minus and Pe. minor , respectively which are classified as monoterpene alcohol dehydrogenases showed specificity towards NADP + as their coenzyme (Hassan et al, 2012; Tan et al, 2019). Other terpene alcohol dehydrogenases isolated from plants, such as N. tabacum allylic alcohol dehydrogenase (Hirata et al, 2000), Salvia officinalis borneol dehydrogenase (Croteau et al, 1978) and Mentha piperita isopiperitenol dehydrogenase (Kjonaas et al, 1985) showed preference to both NADP + or NAD + .…”

“…Several alcohol dehydrogenases from plants such as nerol dehydrogenase from Po. minus (Tan et al, 2019), cultured rice cells alcohol dehydrogenase (Igaue & Yagi, 1982), Triticum turgidum (wheat) alcohol dehydrogenases (Langston et al, 1980) and Vitis vinifera (grape) alcohol dehydrogenases (Tesnière & Verriès, 2000) existed as homodimers. A plant based acyclic monoterpene primary alcohol dehydrogenase from Rauwolfia serpentina (Ikeda et al, 1991) appeared as a monomer in solution.…”

“…T A B L E 2 Substrate specificity with relative activity, K m , V max and V max /K m value for rCcFolDH The results obtained suggested the ability of CcFolDH to clearly distinguish the isomers of substrates, comparable to several insect and plant farnesol dehydrogenases, geraniol dehydrogenases, and nerol dehydrogenase (Ahmad-Sohdi et al, 2015;Hassan et al, 2012;Inoue et al, 1984;Mayoral et al, 2009;Noge et al, 2008;Satyaveanthan et al, 2021;Sekiwa-Iijima et al, 2001;Singh Sangwan et al, 1993;Tan et al, 2019;Zifruddin et al, 2021).…”

“…Several alcohol dehydrogenases from plants such as nerol dehydrogenase from Po. minus (Tan et al, 2019), cultured rice cells alcohol dehydrogenase (Igaue & Yagi, 1982), Triticum turgidum (wheat) alcohol dehydrogenases (Langston et al, 1980) and Vitis vinifera which preferred only NADP + as their coenzyme (Mayoral et al, 2009;Zifruddin et al, 2021). NAD + was selected over NADP + as a coenzyme by a farnesol dehydrogenase isolated from M. sexta (Baker et al, 1983).…”

Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia

“…One the other hand, potential GES of citronellol may decide on the citronellol accumulation on to a greater extent by inducing citronellol synthesis. Whereas, the studies of dehydrogenases of geraniol or nerol are greatly about geraniol acid and haven’t identified the one for citronellol transformation until now ( Hassan et al, 2012 ; Tan et al, 2019a ; Tan et al, 2019b ), much less to the indistinguishable identification of geraniol or citronellol GES which is hidden in ambiguous function of terpenoid synthase family ( Magnard et al, 2015 ). We prefer that the high citronellol accumulation more result from the induced response of an assumed GES.…”