Molecular characterisation of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese

Zayda, Mahmoud Gamaleldin; Masuda, Yoshito; Hammad, Ahmed M.; Honjoh, Ken Ichi; Elbagory, Abdel-Rahman M.; Miyamoto, Takahisa

doi:10.1016/j.idairyj.2020.104646

Cited by 28 publications

(33 citation statements)

References 64 publications

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“…However, oxacillin and amikacin, which were less used in veterinary medicine, still showed drug resistance, which indicates the potential of cross-resistance. Similar to the findings of Meng et al [ 22 ] and Zayda et al [ 28 ], MRSA isolates were found to be resistant to a broader spectrum of antimicrobials than MSSA isolates in the current study. Resistance rates of the MRSA isolates were significantly higher to gentamicin, kanamycin, amikacin, erythromycin, azithromycin and sulfafurazole compared with those of MSSA isolates, attributing to the existence of SCCmec in MRSA strains so that MRSA anchored more resistance genes than MSSA [ 29 , 30 ].…”

Section: Discussionsupporting

confidence: 91%

“…Further comparison of MDR in MRSA and MSSA isolates, MDR was identified in all MRSA isolates and the proportion of multi-drug resistant strains in MRSA was almost three times higher than that of MSSA, which was in line with a previous study [ 31 ]. All these findings indicate the severity of antimicrobial resistance of MRSA isolates, which may be attributed to the increase in affinity of MRSA in acquiring mobile genetic elements (MGEs), such as transposons or conjugative plasmids carrying antimicrobial resistance genes [ 28 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China

Gan

Shu

et al. 2021

BMC Vet Res

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Background Staphylococcus aureus (S. aureus), especially methicillin-resistant Staphylococcus aureus (MRSA), is considered a common zoonotic pathogen, causing severe infections. The objective of this study was to investigate the antimicrobial susceptibility, resistance genes and molecular epidemiology among MRSA and methicillin-susceptible Staphylococcus aureus (MSSA) isolated from food animals in Sichuan Province, China. Methods This study was conducted on 236 S. aureus isolates. All isolates were subjected to antimicrobial susceptibility testing by using a standard microbroth dilution method. The Polymerase Chain Reaction (PCR) was performed to identify genes encoding the β-lactams resistance (blaZ, mecA), macrolides (ermA, ermB, ermC) and aminoglycosides (aacA-aphD). The molecular structures and genomic relatedness of MRSA isolates were determined by staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis (PFGE), respectively. Results Among 236 isolates, 24 (10.17 %) were recognized as MRSA. MRSA isolates showed different resistance rates to 11 antimicrobials ranging from 33.33 to 100 %, while for MSSA isolates the rates varied from 8.02 to 91.51 %. Multi-drug resistance phenotype was found in all MRSA isolates. The ermC gene encoding macrolides-lincosamides-streptogramin B was the most prevalent gene detected in 87.29 % of the S. aureus isolates, followed by ermB (83.05 %), blaZ (63.98 %), aacA-aphD (44.07 %), ermA (11.44 %) and mecA (11.02 %) genes. The prevalence of resistance genes in MRSA isolates was significantly higher than that of MSSA. Regarding the molecular morphology, SCCmec III (12/24, 50 %) was the most common SCCmec type. Furthermore, the PFGE typing showed that 24 MRSA were divided into 15 cluster groups (A to O), the major pulsotype J encompassed 25 % of MRSA isolates. Conclusions The S. aureus isolates from food animals in Sichuan province of China have severe antimicrobials resistance with various resistance genes, especially MRSA isolates. Additionally, the genetic pool of MRSA isolates is diverse and complex, and further investigation is necessary.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China

Gan

Shu

et al. 2021

BMC Vet Res

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“…Staphylococcal protein A types t084, t304 and t688 from subclinical mastitis showed divergent virulence and heterogenicity traits while a novel spa-type t18546 was also reported from dairy udder ailments [35]. Prevalent clonal types of S. aureus from bovine udder exhibited generic alterations of epigenetic modulators to surpass immune response of host.…”

Section: Staphylococcus Aureus Strains Spectrummentioning

confidence: 97%

Staphylococcus aureus and Dairy Udder

Aqib¹,

Ijaz²,

Shoaib³

et al. 2021

Insights Into Drug Resistance in Staphylococcus Aureus

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Staphylococcus aureus is a major causative agent of intra-mammary infections in dairy animals with potential virulence of surface components, toxins, and extracellular enzymes. About 74% quarter prevalence of S. aureus in bovine udder with overall prevalence exceeding 61% in dairy animals. About 17 different serotypes of dairy originated S. aureus have been reported with 24 virulence coding genes for leukocidins (lukED/lukM), pyrogenic toxin super antigen (PTSAg), haemolysins (hla-hlg), toxic-shock syndrome toxin (tst), enterotoxins (sea-seo, seu), exfoliative toxins (eta, etb), and genes for methicillin (mecA) and penicillin (blaZ) resistance. Attainment of refuge inside the macrophages and neutrophils is a major cause of S. aureus mastitis persistence. Mammary prebiotics and probiotics are recently being used as alternatives to antibiotic for the prevention of mastitis. Literature showed anti- staphylococcus vaccines with different results depending upon types of immunization, route of administration and adjuvant used. Studies has shown that herd specific as well as commercial S. aureus vaccines reduce new infections in dairy animals. Experiments are still in progress for the use of vaccines against S. aureus mastitis with optimal efficacy and reliability. Perhaps, there might be bright future because of highly satisfactory trial results of mastitis vaccines in the lab animals.

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“…Contracting MRSA from any of the above sources can occur by consumption or poor handling of contaminated sources [29,38]. Egypt is the largest African dairy producer and among the leading cheese producers in the Mediterranean region, with household animals producing around 80% of cow and buffalo milk [47]. Being a developing country, safety practices necessary to limit transmission of MRSA via dairy products are usually overlooked which poses considerable threat to the public health [47].…”

Section: Evolving Epidemiology Of Mrsamentioning

confidence: 99%

Genomic Overview into the Evolving Epidemiology of Methicillin-Resistant Staphylococcus aureus

dessouky

Mouftah

Elhadidy³

2020

Mansoura Veterinary Medical Journal

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Emerging infections represent an enormous challenge to both human and veterinary medicine. Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) in various species and in food has raised concerns about the roles of animals in the epidemiology of MRSA. MRSA are a group of grampositive bacteria, distinct from other strains of S. aureus in that this pathogen is resistant to methicillin, oxacillin, and all beta-lactam antibiotics. The severity of infections caused by MRSA depends on the strain responsible for the infection and can vary from soft tissue infections to bacteremia and sometimes pneumonia. MRSA strains are divided into clones, based on their genetic makeup. According to the setting of infection, MRSA are divided into three epidemiological types: hospital acquired (HA-MRSA), community acquired (CA-MRSA), and livestock acquired (LA-MRSA) (ie. Transmitted from animal carriers). The epidemiology of HA-MRSA, CA-MRSA, and LA-MRSA is blurred as different recent genetic studies have revealed significant overlap of identical clones between HA-MRSA and CA-MRSA, and the significant increase of human infection caused by LA-MRSA. Furthermore, the animal-human and animal-animal transmission of LA-MRSA has prompted further investigation to study the origin of this epidemiological type and the transmission dynamics. The genetic and virulence profiles of different types of MRSA vary widely, where community acquired and livestock acquired strains are more virulent than hospital acquired strains. This review sheds light on three epidemiological groups of MRSA (HA -MRSA, CA-MRSA, and LA-MRSA), and their most prevalent clonal clusters, that can consequently allow better understanding of their evolution, emergence, transmission, and global dissemination.

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Molecular characterisation of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese

Cited by 28 publications

References 64 publications

Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China

Antimicrobial resistance and genotyping of Staphylococcus aureus obtained from food animals in Sichuan Province, China

Staphylococcus aureus and Dairy Udder

Genomic Overview into the Evolving Epidemiology of Methicillin-Resistant Staphylococcus aureus

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