Molecular Characterisation of M. kansasii Isolates by Whole-Genome Sequencing

Rajendran, Priya; Padmapriyadarsini, Chandrasekaran; Nagarajan, Naveenkumar; Samyuktha, Roja; Govindaraju, Vadivu; Golla, Radhika; Ashokkumar, Shanmugavel; Shanmugam, Sivakumar

doi:10.3390/pathogens12101249

Cited by 2 publications

(2 citation statements)

References 31 publications

(40 reference statements)

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“…Additionally, we have provided new MKC WGS data for isolates from 28 countries, including 10 countries with no MKC available data until now (Bangladesh, Colombia, Estonia, Guadeloupe, Martinique, Pakistan, Romania, Russia, Slovenia, and Sweden). After realizing our experiments, Zhang et al 15 and Rajendran et al 16 published data including MKC genomes from respectively 237 and 12 isolates from China and India.…”

Section: Discussionmentioning

confidence: 99%

Phylogenomic and genomic analysis reveals unique and shared genetic signatures of Mycobacterium kansasii complex species

Machado,

Vasconcellos,

Gomes

et al. 2024

Preprint

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Species belonging to the Mycobacterium kansasii complex (MKC) are frequently isolated from humans and the environment and can cause serious diseases. The most common MKC infections are caused by the species M. kansasii (stricto sensu), leading to tuberculosis-like disease. However, a broad spectrum of virulence, antimicrobial resistance and pathogenicity of these non-tuberculous mycobacteria (NTM) are observed across the MKC. Many genomic aspects of the MKC that relate to these broad phenotypes are not well elucidated. Here, we performed genomic analyses from a collection of 665 MKC strains, isolated from environmental, animal and human sources. We inferred the MKC pangenome, mobilome, resistome, virulome and defense systems and show that the MKC species harbors unique and shared genomic signatures. High frequency of presence of prophages and different types of defense systems was observed. We found that the M. kansasii species splits into four lineages, of which three are lowly represented and mainly in Brazil, while one lineage is dominant and globally spread. Moreover, we show that four sub-lineages of this most distributed M. kansasii lineage emerged during the 20th century. Further analysis of the M. kansasii genomes revealed almost 300 regions of difference contributing to genomic diversity, as well as fixed mutations that may explain the M. kansasii's increased virulence and drug resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Phylogenomic and genomic analysis reveals unique and shared genetic signatures of Mycobacterium kansasii complex species

Machado,

Vasconcellos,

Gomes

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…[52] and Rajendran et al . [53] published data including MKC genomes from respectively 237 and 12 isolates from China and India.…”

Section: Discussionmentioning

confidence: 99%

Phylogenomic and genomic analysis reveals unique and shared genetic signatures of Mycobacterium kansasii complex species

Machado,

Vasconcellos,

Gomes

et al. 2024

Microbial Genomics

View full text Add to dashboard Cite

Species belonging to the Mycobacterium kansasii complex (MKC) are frequently isolated from humans and the environment and can cause serious diseases. The most common MKC infections are caused by the species M. kansasii (sensu stricto), leading to tuberculosis-like disease. However, a broad spectrum of virulence, antimicrobial resistance and pathogenicity of these non-tuberculous mycobacteria (NTM) are observed across the MKC. Many genomic aspects of the MKC that relate to these broad phenotypes are not well elucidated. Here, we performed genomic analyses from a collection of 665 MKC strains, isolated from environmental, animal and human sources. We inferred the MKC pangenome, mobilome, resistome, virulome and defence systems and show that the MKC species harbours unique and shared genomic signatures. High frequency of presence of prophages and different types of defence systems were observed. We found that the M. kansasii species splits into four lineages, of which three are lowly represented and mainly in Brazil, while one lineage is dominant and globally spread. Moreover, we show that four sub-lineages of this most distributed M. kansasii lineage emerged during the twentieth century. Further analysis of the M. kansasii genomes revealed almost 300 regions of difference contributing to genomic diversity, as well as fixed mutations that may explain the M. kansasii’s increased virulence and drug resistance.

show abstract

Molecular Characterisation of M. kansasii Isolates by Whole-Genome Sequencing

Cited by 2 publications

References 31 publications

Phylogenomic and genomic analysis reveals unique and shared genetic signatures of Mycobacterium kansasii complex species

Phylogenomic and genomic analysis reveals unique and shared genetic signatures of Mycobacterium kansasii complex species

Phylogenomic and genomic analysis reveals unique and shared genetic signatures of Mycobacterium kansasii complex species

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