Molecular characterisation of equine group A rotavirus, Nasuno, isolated in Tochigi Prefecture, Japan

Fukai, Katsuhiko; Saito, Toshiya; Fukuda, Osamu; Hagiwara, Atsuko; Inoue, Katsumi; Satõ, Mitsuo

doi:10.1016/j.tvjl.2005.05.004

Cited by 13 publications

(18 citation statements)

References 11 publications

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“…For amplification of the VP8* subunit of the VP4 gene, the primers Con2 and Con3 were used (20) (Table 1), applying the same thermal conditions as those used for amplification of the VP7 gene. Prediction of the VP4 (P) type was accomplished using a pool of primers, including the P[12]-and P[18]-specific oligonucleotides described by Fukai et al (19) and primer Con3 (20). Identical reaction and thermal conditions were used as those described for the G typing PCR.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Equine Rotavirus in Ireland

et al. 2008

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Group A rotaviruses are important causative agents of severe, acute dehydrating diarrhea in foals. A total of 86 rotavirus-positive fecal samples, collected from diarrheic foals from 11 counties in three of the four provinces of Ireland, were obtained from the Irish Equine Centre in Kildare during a 7-year (1999 to 2005) passive surveillance study and were characterized molecularly to establish the VP7 (G type) and VP4 (P type) antigenic specificities. Fifty-eight samples (67.5%) were found to contain G3 viruses, while in 26 samples (30.2%) the rotaviruses were typed as G14 and in 2 samples (2.3%) there was a mixed infection, G3 plus G14. All samples except for two, which were untypeable, were characterized as P

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Equine Rotavirus in Ireland

et al. 2008

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“…Virus isolation and serological tests are not routinely available in diagnostic laboratories because they require a sophisticated technique and take a long time. Molecular diagnostic methods, such as a reverse transcription-polymerase chain reaction (RT-PCR), can provide a result within several hours [6,8,9,21]. However, RT-PCR requires expensive equipment, and this precludes the use of RT-PCR for diagnosis in some laboratories.…”

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confidence: 99%

“…The details of the primers used for the RT-LAMP assay are listed in Table 1. The reaction mixture was prepared using a Loopamp Semi-nested RT-PCR was performed with the primers designed to target the VP4 gene of the genotype P[12] strain described by Fukai et al [9]. The viral RNA was heated at 95C for 5 min and then immediately cooled on ice.…”

mentioning

confidence: 99%

Detection of Equine Rotavirus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

Nemoto

Imagawa

Tsujimura

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to detection of equine rotavirus. Because equine rotavirus of the single P genotype, P [12], is predominant in the equine population worldwide, an RT-LAMP primer set was designed to target the genotype P[12] sequence and thus detect equine rotavirus. The detection limit of the RT-LAMP assay was 10 3 copies of viral RNA, whereas that of semi-nested RT-PCR for genotype P[12] was 10 5 copies. The RT-LAMP assay specifically amplified genotype P[12] but did not amplify the other P genotype strains. The RT-LAMP assay did not amplify any pathogens related to equine intestinal disorder other than rotavirus. Using 96 diarrheal stools, the RT-LAMP assay detected equine rotavirus in 58 samples, whereas semi-nested RT-PCR only detected equine rotavirus in 25 samples. The RT-LAMP assay did not detect equine rotavirus with fecal samples collected from nine healthy foals. These results indicate that the RT-LAMP assay is specific for equine rotavirus and more sensitive than semi-nested RT-PCR. Because it is easy to manipulate without the need for a thermal cycler or gel electrophoresis, the RT-LAMP assay should be applicable to diagnosis of equine rotavirus infections in diagnostic laboratories.KEY WORDS: equine rotavirus, reverse transcription loop-mediated isothermal amplification (RT-LAMP).

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“…During three decades there have been several studies in Japan leading to the genetic analysis and characterization of equine RVAs (Takagi et al, 1994;Tsunemitsu et al, 2001;Fukai et al, 2006;Nemoto et al, 2012;). Till 2013 only few reports for the whole genome characterization and analysis of equine RVA strains have been documented (Ghosh et al, 2012;Matthijnssens et al, 2012b;Mino et al, 2013).…”

Section: Rotavirusesmentioning

confidence: 99%

Evolving views on enteric viral infections of equines: an appraisal of key pathogens

Sircar¹,

Saurabh²,

Kattoor³

et al. 2016

JEBAS

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Equines, the earliest known mammalian species, have been found highly susceptible to several enteric pathogens including viruses, fungi, parasites and bacteria. This review conserves with the key viral pathogens that affects foals and horses such as rotavirus, adenovirus, coronavirus, parvovirus, picobirnavirus etc. With the advent of next generation sequencing approaches the list of new enteric viruses has expanded. Viruses like Cyclovirus, Kirkovirus and Anellovirus are the new members identified in equines recently. Close proximity of horses to human settlements and/or other domestic animals pretense the threat of infectious diseases spread to humans/animals and vice-versa. Therefore, horse diseases are not only of veterinary importance but also are among important factors for public health. Herein, we intend to appraise current status of key enteric viruses that cause diarrheic disorders in foals and horses.

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Molecular characterisation of equine group A rotavirus, Nasuno, isolated in Tochigi Prefecture, Japan

Cited by 13 publications

References 11 publications

Molecular Characterization of Equine Rotavirus in Ireland

Molecular Characterization of Equine Rotavirus in Ireland

Detection of Equine Rotavirus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

Evolving views on enteric viral infections of equines: an appraisal of key pathogens

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