Molecular characterisation of

Feng, Yating; Huang, Qiaoli; Zhang, Rui; Li, Junyi; Luo, Kai; Chen, Yinhua

doi:10.1071/cp21558

Cited by 12 publications

(6 citation statements)

References 56 publications

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“…Several data sources suggest that overexpression of specific PAL gene members can activate the defense mechanisms and promote the functional improvement of plants under abiotic stresses. For instance, salinity stress was reported to induce the upregulation of PAL6 in the roots of Medicago sativa [63] and PAL1 in leaves of two Salvia species, consequently increasing total phenolic accumulation [64]. Thus, our results confirm PAL as an indicator of stress conditions in plant species.…”

Section: Discussionsupporting

confidence: 79%

Integrated Metabolomics and Morpho-Biochemical Analyses Reveal a Better Performance of Azospirillum brasilense over Plant-Derived Biostimulants in Counteracting Salt Stress in Tomato

Zuluaga

Miras-Moreno

Monterisi

et al. 2022

IJMS

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Increased soil salinity is one of the main concerns in agriculture and food production, and it negatively affects plant growth and crop productivity. In order to mitigate the adverse effects of salinity stress, plant biostimulants (PBs) have been indicated as a promising approach. Indeed, these products have a beneficial effect on plants by acting on primary and secondary metabolism and by inducing the accumulation of protective molecules against oxidative stress. In this context, the present work is aimed at comparatively investigating the effects of microbial (i.e., Azospirillum brasilense) and plant-derived biostimulants in alleviating salt stress in tomato plants by adopting a multidisciplinary approach. To do so, the morphological and biochemical effects were assessed by analyzing the biomass accumulation and root characteristics, the activity of antioxidant enzymes and osmotic stress protection. Furthermore, modifications in the metabolomic profiles of both leaves and root exudates were also investigated by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS). According to the results, biomass accumulation decreased under high salinity. However, the treatment with A. brasilense considerably improved root architecture and increased root biomass by 156% and 118% in non-saline and saline conditions, respectively. The antioxidant enzymes and proline production were enhanced in salinity stress at different levels according to the biostimulant applied. Moreover, the metabolomic analyses pointed out a wide set of processes being affected by salinity and biostimulant interactions. Crucial compounds belonging to secondary metabolism (phenylpropanoids, alkaloids and other N-containing metabolites, and membrane lipids) and phytohormones (brassinosteroids, cytokinins and methylsalicylate) showed the most pronounced modulation. Overall, our results suggest a better performance of A. brasilense in alleviating high salinity than the vegetal-derived protein hydrolysates herein evaluated.

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Section: Discussionsupporting

confidence: 79%

Integrated Metabolomics and Morpho-Biochemical Analyses Reveal a Better Performance of Azospirillum brasilense over Plant-Derived Biostimulants in Counteracting Salt Stress in Tomato

Zuluaga

Miras-Moreno

Monterisi

et al. 2022

IJMS

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“…Members of the PAL gene family have been identified and extensively studied in many plants. The PAL gene family typically comprises 4–15 members, which varies between species, including alfalfa, 11 walnut, 35 potato, 6 and grape. 12 The small difference in the number of members in the PAL gene family indicates that PAL genes did not undergo significant amplification during species differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Different PAL family members contain different numbers and types of elements, indicating that GbPAL genes are involved in different biological regulatory processes, and different PAL genes have specific regulatory patterns. Many studies have shown that PAL genes are involved in responses to environmental stimuli, including stress conditions such as UV exposure, 1 drought, 38 waterlogging, 11 pathogens, wounds, and extreme temperatures. Individual PAL proteins may be structurally distinct from other PALs and appear to have different functions, making PAL a multifunctional gene family.…”

Section: Discussionmentioning

confidence: 99%

“…The deciphering of more plant genomes has provided a scientific foundation for exploring the PAL gene family. The PAL gene family has been extensively studied in several plants, such as rice, 10 Medicago sativa , 11 Vitis vinifera , 12 and Cuminum cyminum . 13 However, the number of PAL gene family members varies significantly among different plants, with higher gene copy numbers in potatoes and lower gene copy numbers in A. thaliana , Oryza sativa , V. vinifera , and N. tabacum .…”

Section: Introductionmentioning

confidence: 99%

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Expression profiling of the phenylalanine ammonia-lyase ( PAL ) gene family in ginkgo biloba L.

Gao,

Hu,

et al. 2023

Plant Signaling & Behavior

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The PAL gene family plays an important role in plant growth, development, and response to abiotic stresses and has been identified in a variety of plants. However, a systematic characterization is still lacking in Ginkgo biloba . Using a bioinformatics approach, 11 GbPAL members of the PAL gene family identified in ginkgo were identified in this study. The protein structure and physicochemical properties indicated that the GbPAL genes were highly similar. Based on their exon-intron structures, they can be classified into three groups. A total of 62 cis-elements for hormone, light, and abiotic stress responses were identified in the promoters of GbPAL genes, indicating that PAL is a multifunctional gene family. GbPAL genes were specifically expressed in different tissues and ploidy of ginkgo. These results provide a theoretical basis for further studies on the functional expression of the GbPAL genes.

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“…Phenylalanine ammonia-lyase ( PAL ) is a key enzyme in the phenylalanine metabolic pathway of plants [ 9 ]. It plays a crucial role in plant growth, development and resistance to biotic and abiotic stresses [ 10 – 12 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of PAL genes involved in the regulation of stem development in Saccharum spontaneum L.

Wu,

Cui,

et al. 2024

BMC Genom Data

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Background Saccharum spontaneumL. is a closely related species of sugarcane and has become an important genetic component of modern sugarcane cultivars. Stem development is one of the important factors for affecting the yield, while the molecular mechanism of stem development remains poorly understanding in S. spontaneum. Phenylalanine ammonia-lyase (PAL) is a vital component of both primary and secondary metabolism, contributing significantly to plant growth, development and stress defense. However, the current knowledge about PAL genes in S. spontaneum is still limited. Thus, identification and characterization of the PAL genes by transcriptome analysis will provide a theoretical basis for further investigation of the function of PAL gene in sugarcane. Results In this study, 42 of PAL genes were identified, including 26 SsPAL genes from S. spontaneum, 8 ShPAL genes from sugarcane cultivar R570, and 8 SbPAL genes from sorghum. Phylogenetic analysis showed that SsPAL genes were divided into three groups, potentially influenced by long-term natural selection. Notably, 20 SsPAL genes were existed on chromosomes 4 and 5, indicating that they are highly conserved in S. spontaneum. This conservation is likely a result of the prevalence of whole-genome replications within this gene family. The upstream sequence of PAL genes were found to contain conserved cis-acting elements such as G-box and SP1, GT1-motif and CAT-box, which collectively regulate the growth and development of S. spontaneum. Furthermore, quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that SsPAL genes of stem had a significantly upregulated than that of leaves, suggesting that they may promote the stem growth and development, particularly in the + 6 stem (The sixth cane stalk from the top to down) during the growth stage. Conclusions The results of this study revealed the molecular characteristics of SsPAL genes and indicated that they may play a vital role in stem growth and development of S. spontaneum. Altogether, our findings will promote the understanding of the molecular mechanism of S. spontaneum stem development, and also contribute to the sugarcane genetic improving.

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Molecular characterisation of

Cited by 12 publications

References 56 publications

Integrated Metabolomics and Morpho-Biochemical Analyses Reveal a Better Performance of Azospirillum brasilense over Plant-Derived Biostimulants in Counteracting Salt Stress in Tomato

Integrated Metabolomics and Morpho-Biochemical Analyses Reveal a Better Performance of Azospirillum brasilense over Plant-Derived Biostimulants in Counteracting Salt Stress in Tomato

Expression profiling of the phenylalanine ammonia-lyase ( PAL ) gene family in ginkgo biloba L.

Identification and characterization of PAL genes involved in the regulation of stem development in Saccharum spontaneum L.

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