Molecular characterisation of dominant repressors of the cytokinin deficiency syndrome

Jensen, Helen

doi:10.17169/refubium-6507

Cited by 1 publication

(3 citation statements)

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“…The pAHK3:ROCK3 gene has been described [ 31 ]. To generate pIPT3:ROCK4 , ~2000 bp of the A. thaliana gene IPT3 (At3g63110) were combined with the coding sequence of the mutated gene IPT3 named ROCK4 [ 25 ]. Oligonucleotides used for gene amplification and cloning are listed in Supplemental Table S1 .…”

Section: Methodsmentioning

confidence: 99%

“…Several genes possessing enhanced cytokinin synthesis or signaling activity have been isolated during a suppressor screen searching for mutants reverting the phenotypic consequences of cytokinin deficiency in Arabidopsis. One gene, named REPRESSOR OF CYTOKININ DEFICIENCY4 ( ROCK4 ), carries a mutation in the IPT3 gene, rendering the encoded cytokinin-synthesizing IPT3 protein more active [ 25 ]. A second gene identified in that suppressor screen, ROCK3 , carries a mutation in the ligand-binding domain of the ARABIDOPSIS HISTIDINE KINASE3 (AHK3) cytokinin receptor, causing its constitutive activation [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, ROCK4 can be used to increase cytokinin biosynthesis and ROCK3 to achieve cell-autonomous enhanced cytokinin signaling. In Arabidopsis, the expression of the ROCK4 and ROCK3 genes under control of their own promoter led to a significant increase of the cell number of interfascicular cambium, and together with an increased cell size, an up to 50% greater stem diameter [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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A Constitutively Active Cytokinin Receptor Variant Increases Cambial Activity and Stem Growth in Poplar

Riefler

Brügmann²,

Fladung³

et al. 2022

IJMS

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The cambial meristem is responsible for bark and wood formation in woody plants. The activity of the cambial meristem is controlled by various factors; one of them is the plant hormone cytokinin. Here, we have explored different approaches to genetically engineering cambial activity in poplar plants by the ectopic expression of a cytokinin biosynthesis gene with enhanced activity (named ROCK4) or of a gene encoding a constitutively active cytokinin receptor variant (ROCK3). Both genes are derived from Arabidopsis thaliana and were expressed in poplar trees under the control of their own promoter or the cambium-specific pHB8 promoter. pIPT3:ROCK4- and pHB8:ROCK4-expressing plants were smaller than wild-type plants and formed more lateral branches; pHB8:ROCK4 transgenic plants additionally showed an increased stem diameter. In contrast, pAHK3:ROCK3- and pHB8:ROCK3-expressing plants grew taller than wild type without an altered branching pattern and formed more cambial cells, leading to increased radial stem growth. The effectivity of ROCK3 when expressed in either secondary phloem cells or in cambial cells is consistent with a dual, tissue-autonomous and non-autonomous activity of cytokinin in regulating cambial activity. We propose ROCK3 as a novel gene to enhance biomass formation in woody plants.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%