Molecular characterisation of a novel cassava associated circular ssDNA virus

Dayaram, Anisha; Opong, Allen; Jäschke, Anja; Hadfield, James; Baschiera, Marianna; Dobson, Renwick C. J.; Offei, Samuel Kwame; Shepherd, Dionne N.; Martin, Darren P.; Varsani, Arvind

doi:10.1016/j.virusres.2012.03.009

Cited by 55 publications

(30 citation statements)

References 43 publications

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“…Furthermore, many SsHADV-1-like viruses were recently found in other ecological niches (23)(24)(25)(26). Surprisingly, abundant viruses with high similarity to SsHADV-1 were identified in the nearsurface atmosphere and were proposed to be of fungal origin (27). Moreover, SsHADV-1 Rep gene-like sequences have been found integrated in the genomes of many fungi, including the rice blast fungus Magnaporthe oryzae and the mushrooms Tuber melanosporum and Laccaria bicolor (28).…”

Section: Discussionmentioning

confidence: 99%

Extracellular transmission of a DNA mycovirus and its use as a natural fungicide

Fù

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

234

170

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Mycoviruses are thought not to be infectious as free particles and to lack an extracellular phase in their life cycles, limiting the broad use of hypovirulence-associated mycoviruses in controlling fungal disease. Here, we demonstrate that purified particles of a DNA mycovirus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), are infectious when applied extracellularly to its host Sclerotinia sclerotiorum. Virus particles isolated from an infected host can infect the hyphae of virus-free S. sclerotiorum directly when applied to hyphae grown on potato dextrose agar or sprayed on leaves of Arabidopsis thaliana and Brassica napus, regardless of vegetative compatibility affiliation. When applied to leaves, the virus can suppress the development of lesions. SsHADV-1 can also reduce disease severity and enhance rapeseed yield significantly under field conditions. SsHADV-1 has a narrow host range; it can infect Sclerotinia minor and Sclerotinia nivalis, sister species of S. sclerotiorum, and cause debilitation of these two fungi, but cannot infect or transfect other tested fungi, such as Botrytis cinerea, which shares the same family with S. sclerotiorum. Virus particles are likely to be very stable on the leaves of A. thaliana plants because viral DNA could be detected at 15 d postinoculation on unwounded leaves and at 10 d postinoculation on wounded leaves, respectively; however, this virus could not infect and move in plant cells. Our findings may prompt a reconsideration of the generalization that mycoviruses lack an extracellular phase in their life cycles and stimulate the search for other DNA mycoviruses with potential use as natural fungicides.single-stranded DNA virus | biological control | crop fungal disease | rapeseed stem rot

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Section: Discussionmentioning

confidence: 99%

Extracellular transmission of a DNA mycovirus and its use as a natural fungicide

Fù

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

234

170

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“…2a). An intron lies within the rep gene, which is similar to those in some geminiviruses [7,16]. Furthermore, this gemycircularvirus has an additional putative large ORF enconding a 200aa protein with the same direction as Rep ORF (Fig.…”

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confidence: 85%

“…Recently, a group of novel gemycircularviruses have been identified from the feces of various animals [3,4], fungus [2], insect body [1,5], sewage [3,6], plant [7][8][9], and human [10,11]. Although no report indicates gemycircularvirus is associated with a certain disease, reports isolated this virus from the blood of a patient with multiple sclerosis [10] and the cerebrospinal fluid (CSF) from a patient with encephalitis [11].…”

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confidence: 99%

A novel gemycircularvirus from experimental rats

Shen

et al. 2015

Virus Genes

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Recently, gemycircularviruses have been found in humans and various species of animals. Here, a novel gemycircularvirus named Ch-zjrat-01 from blood samples of experimental rats was characterized. The novel gemycircularvirus encodes two major proteins, including a capsid protein (Cap) and a replication-associated protein (Rep). Phylogenetic analysis based on the amino acid sequence of Rep indicated that Ch-zjrat-01 clusters with two gemycircularviruses discovered from bird (KF371635) and mosquito (HQ335086), sharing 48.7 and 49.4 % sequence identities with them, respectively.

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“…3 Maximum-likelihood (ML) phylogenetic trees of the Rep and CP amino acid sequences of representative geminiviruses. The Rep ML phylogenetic tree has been rooted with Rep sequences of cassavaassociated circular DNA virus (JQ412057) [9] and Sclerotinia sclerotiorum hypovirulence-associated virus-1 (GQ365709) [21,40], whose Rep sequences are distantly related to those of geminiviruses [32]. The CP tree has simply been rooted at the midpoint along the branch path separating the two most divergent sequences represented New genera in the family Geminiviridae 2199 species have been found infecting monocotyledonous plants of the species Eragrostis curvula (weeping love grass) in the Kwa-Zulu Natal region of South Africa (Table 2) [35].…”

Section: Eragrovirusmentioning

confidence: 99%

Establishment of three new genera in the family Geminiviridae: Becurtovirus, Eragrovirus and Turncurtovirus

et al. 2014

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The family Geminiviridae includes plantinfecting circular single-stranded DNA viruses that have geminate particle morphology. Members of this family infect both monocotyledonous and dicotyledonous plants and have a nearly global distribution. With the advent of new molecular tools and low-cost sequencing, there has been a significant increase in the discovery of new geminiviruses in various cultivated and non-cultivated plants.In this communication, we highlight the establishment of three new genera (Becurtovirus, Eragrovirus and Turncurtovirus) to accommodate various recently discovered geminiviruses that are highly divergent and, in some cases, have unique genome architectures. The genus Becurtovirus has two viral species, Beet curly top Iran virus (28 isolates; leafhopper vector Circulifer haematoceps) and Spinach curly top Arizona virus (1 isolate; unknown vector), whereas the genera Eragrovirus and Turncurtovirus each have a single assigned species: Eragrostis curvula streak virus (6 isolates; unknown vector) and Turnip curly top virus (20 isolates; leafhopper vector Circulifer haematoceps), respectively. Based on analysis of all of the genome sequences available in public databases for each of the three new genera, we provide guidelines and protocols for species and strain classification within these three new genera.Electronic supplementary material The online version of this article

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Molecular characterisation of a novel cassava associated circular ssDNA virus

Cited by 55 publications

References 43 publications

Extracellular transmission of a DNA mycovirus and its use as a natural fungicide

Extracellular transmission of a DNA mycovirus and its use as a natural fungicide

A novel gemycircularvirus from experimental rats

Establishment of three new genera in the family Geminiviridae: Becurtovirus, Eragrovirus and Turncurtovirus

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