Molecular characterisation, genotyping and survival of Aeromonas hydrophila isolated from milk, dairy products and humans in Egypt

Tahoun, Asmaa B.M.B.; Ahmed, Heba A.; Elez, Rasha M.M. Abou; El-Gedawy, Attia; Elsohaby, Ibrahim; El-Ghafar, Abeer E Abd

doi:10.1016/j.idairyj.2016.07.011

Cited by 13 publications

(10 citation statements)

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“…Microbial growth and metabolism which result in the formation of amines, sulfides, alcohols, aldehydes, ketones, and organic acids with unacceptable off‐flavors are the major cause of food spoilage (Gram & Dalgaard, ). Aeromonas species are widely distributed in environment including raw and processed drinking water, and animal feces (Igbinosa, Igbinosa, & Okoh, ; Latif‐Eugenín, Beaz‐Hidalgo, & María José, ; Sen & Rodgers, ), and have been frequently isolated from various food products, such as fish and shellfish, meats and poultry, vegetables, raw milk, and dairy products (Odeyemi & Ahmad, ; Osman, Aly, Kheader, & Mabrok, ; Poojary, ; Tahoun et al, ). Motile aeromonads are considered as emerging foodborne pathogens because it is shown that some Aeromonas isolates from food can produce different virulence factors including cytotoxic enterotoxin, hemolysin, the secretory system, and a variety of extracellular hydrolytic enzymes such as lipases, proteases, and DNase, which are involved in the degradation of host components (Janda & Abbott, ; Singh, Rathore, Kapoor, Mishra, & Lakra, ; Yano et al, ) and might lead to food spoilage as well.…”

Section: Introductionmentioning

confidence: 99%

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

et al. 2017

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This study characterized the spoilage potential of the N-acyl homoserine lactone (AHL)-producing strain, Aeromonas veronii bv. veronii, isolated from fermented surimi, and investigated the effects of exogenous AHLs on its spoilage characteristics. The strain showed a strong ability of producing total volatile base nitrogen (TVB-N) and putrescine, and four types of AHLs, including N-butanoyl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-heptanoyl-Lhomoserine lactone (C7-HSL), and N-octanoyl-L-homoserine lactone (C8-HSL), were identified from the supernatant by LC-MS/MS. Concentrations of AHLs reached the maximum value as the cells entered stationary phase contents and then decreased rapidly with the increasing pH. C8-HSL was found to be dominant with contents of 1,536.33 ng/mL and exogenous C8-HSL was most effective in promoting bacterial growth, biofilm formation, swimming motility, production of TVB-N and putrescine, and relative genes expression. The results indicated the involvement of AHL-based quorum sensing system in the regulation of spoilage potential of Aeromonas spp. Practical applicationsThe results of the present study found that Aeromonas veronii bv. veronii was capable of producing four types of AHLs, which were considered to be involved in the regulation of spoilage potential of the strain. This would enrich the understanding of quorum sensing system in Aeromonas and give clue for new strategy of controlling food spoilage in food industry. K E Y W O R D SAeromonas veronii bv. veronii, homoserine lactone, regulation, quorum sensing, surimi, spoilage potential

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Section: Introductionmentioning

confidence: 99%

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

et al. 2017

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“…Furthermore, Aeromonas spp. isolates were grouped in 5 clusters and 9 single isolates with D value of 0.995 in a study reported by Tahoun et al [32] in Egypt to discriminate between A. hydrophila isolates originated from milk, dairy products and diarrheic human stool samples. They recommended RAPD technique for Aeromonas spp.…”

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confidence: 99%

Aeromonas hydrophila from Fish and Humans: Biofilm Formation and Genetic Relatedness

Mohamed

Ahmed

Rizk³

et al. 2019

Zagazig Veterinary Journal

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A comprehensive study was done to determine the genetic relatedness and the biofilm formation ability of Aeromonas hydrophila strains (n=25) isolated from fish (150 tilapia and 144 mugil) and human stool samples (n=102). The results revealed that Random Amplification of Polymorphic DNA (RAPD-PCR) classified A. hydrophila strains into seven distinct profiles (R1-R7), the amplicon sizes ranged from 183-2930 bp. The isolates were grouped into five main clusters, the presence of isolates from fish and humans in the same cluster indicates the possibility of cross contamination. Biofilm results showed that 96% of A. hydrophila isolates were biofilm producers. At 35ºC, 16 (64%) and 8 (32%) showed strong and moderate biofilm production ability, respectively. At 25ºC, 21 (84%) were biofilm producers, of which, 8 (32%), 7 (28%) and 6 (24%) were strong, moderate and weak, respectively. At 4ºC, decreased biofilm production ability was noticed 13 (52%), where 8 (32%) and 5 (20%) were moderate and weak biofilm producers, respectively. Significant correlation was showed between A. hydrophila isolates and different cultivation temperatures. This clarified the potential virulence of A. hydrophila isolates from both fish and human sources and their public health hazard.

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“…The colony counts of Aeromonas hydrophila and Pseudomonsa putida in macrobrachium rosenbergii were shown in Figure c,d during the storage. The optimal culture temperatures of Aeromonas hydrophila and Pseudomonsa putida were from 25 to 30°C (Israr et al, ; Tahoun et al, ). Because of the low storage temperature and antimicrobial activity by PVA films incorporated with nano‐TiO 2 , the colony counts of these two microorganisms appeared fluctuation in the early storage of 0–2 days.…”

Section: Resultsmentioning

confidence: 99%

Development of antimicrobial active films based on poly(vinyl alcohol) containing nano-TiO₂and its application inmacrobrachium rosenbergiipackaging

Tang

Chen

Xie

2018

J Food Process Preserv

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The antimicrobial poly(vinyl alcohol) (PVA) films incorporated with nano‐TiO2 were prepared by solution blending‐casting method. The effects of different nano‐TiO2 addition of 0%, 0.5%, 1%, 3%, 5%, and 7% on the characterization of PVA films were studied. The tensile strength of the film incorporated with 0.5% nano‐TiO2 was highest. Compared with the pure PVA film, the oxygen transmission rate, swelling ratio and water vapor uptake ratio of the films containing 0.5%–7% nano‐TiO2 reduced 0.4%–0.8%, 22.82%–81.79%, and 3.59%–10.7%, respectively. The transparency reduced significantly. The thermal stability and antimicrobial activity against Staphylococcus aureus and Escherichia coli were improved. The storage study of macrobrachium rosenbergii indicated that the Shewanella spp., Pseudomonas putida and Aeromonas hydrophila could be inhibited by PVA films added with nano‐TiO2 and their shelf‐life was extended for 1–2 days. It was indicated that there's a good prospect for application of these PVA films in the antimicrobial food packaging. Practical applications Poly(vinyl alcohol) (PVA) is a biodegradability and nontoxicity synthetic polymer, which has been widely used in food packaging materials. This paper proved that the PVA films incorporated with nano‐TiO2 showed good antimicrobial activity. For the application in macrobrachium rosenbergii packaging, it showed that the Shewanella spp., Pseudomonas putida and Aeromonas hydrophila could be inhibited by PVA films added with nano‐TiO2 and their shelf‐life could be extended for 1–2 days. There is a great advantage that the antimicrobial activity of the films could be motivated by UV‐light irradiating before packaging process to avoid that the common active packaging films are in the excited state after prepared. This antimicrobial packaging film could be used as inner layer of the composite packaging film to improve the food quality and prolong the shelf‐life of the food.

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Molecular characterisation, genotyping and survival of Aeromonas hydrophila isolated from milk, dairy products and humans in Egypt

Cited by 13 publications

References 46 publications

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

Aeromonas hydrophila from Fish and Humans: Biofilm Formation and Genetic Relatedness

Development of antimicrobial active films based on poly(vinyl alcohol) containing nano-TiO₂and its application inmacrobrachium rosenbergiipackaging

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Molecular characterisation, genotyping and survival of Aeromonas hydrophila isolated from milk, dairy products and humans in Egypt

Cited by 13 publications

References 46 publications

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

Involvement of bacterial quorum sensing signals in spoilage potential ofAeromonas veroniibv.veroniiisolated from fermented surimi

Aeromonas hydrophila from Fish and Humans: Biofilm Formation and Genetic Relatedness

Development of antimicrobial active films based on poly(vinyl alcohol) containing nano-TiO2and its application inmacrobrachium rosenbergiipackaging

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Development of antimicrobial active films based on poly(vinyl alcohol) containing nano-TiO₂and its application inmacrobrachium rosenbergiipackaging