Molecular characterisation, evolution and expression analysis of g-type lysozymes in Ciona intestinalis

Abstract

a b s t r a c tLysozyme is an important defense molecule of the innate immune system. Known for its bactericidal properties, lysozyme catalyzes the hydrolysis of b-(1,4)-glycosidic bonds between the N-acetyl glucosamine and N-acetyl muramic acid in the peptidoglycan layer of bacterial cell walls. In this study, the complete coding sequence of four g-type lysozymes were identified in Ciona intestinalis. Phylogenetic analysis and modelling supported the hypothesis of a close relationship with the vertebrate g-type lysozymes suggesting that the C. intestinalis g-type lysozyme genes (CiLys-g1, Cilys-g2, CiLys-g3, CiLys-g4) share a common ancestor in the chordate lineage. Protein motif searches indicated that C. intestinalis gtype lysozymes contain a GEWL domain with a GXXQ signature, typical of goose lysozymes. Quantitative Real-Time PCR analysis results showed that transcripts are expressed in various tissues from C. intestinalis. In order to determine the involvement of C. intestinalis g-type lysozymes in immunity, their expression was analyzed in the pharynx, showing that transcripts were significantly up-regulated in response to a challenge with lipopolysaccharide (LPS). These data support the view that CiLys g-type are molecules with potential for immune defense system against bacterial infection.

Identification and expression analysis of c-type and g-type lysozymes genes after Aeromonas hydrophila infection in African catfish

“…chicken-type (c-Lyz) and goose-type (g-Lyz) (Callewaert & Michiels, 2010;Callewaert et al, 2012;Zhang et al, 2018). The c-and g-type of lysozymes have been demonstrated to play important roles in the immune defense in many fish species, especially in protection against pathogenic bacterial infection (Liu et al, 2016;Wang et al, 2016;Di Falco et al, 2017;Gou et al, 2018).…”

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

“…Furthermore, some kinds of C‐type lysozymes were also involved in shrimp antiviral response (Karthik et al, ; Mai & Wang, ). The lysozymes also had other functions, such as antitumour, anti‐inflammatory, digestion, immune‐enhancing and feed preservation (Di Falco, Cammarata, & Vizzini, ; Irwin, ; Lee, Ku, Na, & Bae, ). Indeed, the lysozymes may act as a multipurpose defence factor and play vital roles in the immune system.…”

Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

“…Both lysozymes gene has been demonstrated to play essential roles as the immune defense in many fish species (Mohanty dan Sahoo 2010;Pridgeon et al 2013;Wei et al 2014;Di Falco et al 2017). The involvement of the LYSC in the protection against bacterial infection has been reported in fish species, such as resistant families of Atlantic salmon against Piscirickettsia salmonis infection (Dettleff et al 2015), channel catfish against Edwardsiella ictaluri (Wang et al 2013), Darby's sturgeon and Qihe crucian carp against A. hydrophila (Wang et al 2016;Zhang et al 2018), Moreover, the single nucleotide polymorphisms (SNPs) in LYSC gene have been characterized in several fish species, such as Asian seabass (Fu et al 2013) and Japanese flounder (Liu et al 2017) and identified as potential markers for bacterial-resistance in fish.…”